Modification of the amino acid sequence of a phenylpyruvate decarboxylase from Azospirillum brasilense produces a novel group of phenylpyruvate decarboxylases with improved specificity to certain substrates, including in particular C7-C11 2-ketoacids such as, for example, 2-ketononanoate and 2-keto-octanoate. This specificity enables effective use of the phenylpyruvate decarboxylase in, for example, an in vivo process wherein 2-ketobutyrate or 2-ketoisovalerate are converted to C7-C11 2-ketoacids, and the novel phenylpyruvate decarboxylase converts the C7-C11 2-ketoacid to a C6-C10 aldehyde having one less carbon than the 2-ketoacid. Ultimately, through contact with additional enzymes, such C6-C10 aldehydes may be converted to, for example, C6-C10 alcohols, C6-C10 carboxylic acids, C6-C10 alkanes, and other derivatives. Use of the novel genetically modified phenylpyruvate de carboxylases may represent a lower cost alternative to non-biobased approaches.

Systems and methods to provide renewable transportation fuels for internal combustion engines by converting renewable feedstocks into two or more intermediate hydrocarbon blend stocks and blending at least two of the two or more intermediate hydrocarbon blend stocks to produce the renewable transportation fuel. Methods and/or processes may include selecting sugar from a sugar source and introducing the sugar into one or more reactors. The sugar may be converted into an intermediate renewable hydrocarbon blend stock and sent to a separation unit to separate out an intermediate renewable gasoline unit. The process may include selecting and converting a lipid from a lipid source into a renewable diesel product. The renewable diesel product may be sent to a second separation unit to separate out renewable diesel and a low-grade naphtha. The low-grade naphtha and intermediate renewable gasoline may be blended to define a finished renewable gasoline.

A process and system for separating bio-gasoline, bio-diesel and bio-fuel oil fractions from a bio-oil, and for producing a renewable gasoline including at least in part the bio-gasoline fraction, is provided. The process comprises separating bio-oil into a bio-gasoline fraction and a heavy fraction based on their boiling points. At least a portion of the bio-gasoline fraction is directly blended with a petroleum-derived gasoline, without any prior hydrotreatment, to thereby provide a renewable gasoline composition.

The present disclosure generally relates to systems and methods utilizing regenerative agriculture for the procurement, production, refinement and/or transformation of low carbon intensity transportation fuels, including low carbon intensity biodiesel and/or renewable diesel, low carbon intensity biogasoline, low carbon intensity aviation, marine and kerosene fuels as well as fuel oil blends, low carbon intensity ethanol, and low carbon intensity hydrogen, that may be beneficially commercialized directly to consumers. In further aspects, the systems and methods of the present disclosure advantageously generate low carbon intensity comestibles, including sustainably-sourced meal and/or feed. The disclosed systems and methods may be utilized and optimized such that the resulting fuels and foodstuffs are characterized by a reduction in greenhouse gas production and a diminution in the fertilizer, pesticide and water required for producing the associated crop feedstocks.

The present disclosure generally relates to systems and methods utilizing regenerative agriculture for the procurement, production, refinement and/or transformation of low carbon intensity transportation fuels, including low carbon intensity biodiesel and/or renewable diesel, low carbon intensity biogasoline, low carbon intensity aviation, marine and kerosene fuels as well as fuel oil blends, low carbon intensity ethanol, and low carbon intensity hydrogen, that may be beneficially commercialized directly to consumers. In further aspects, the systems and methods of the present disclosure advantageously generate low carbon intensity comestibles, including sustainably-sourced meal and/or feed. The disclosed systems and methods may be utilized and optimized such that the resulting fuels and foodstuffs are characterized by a reduction in greenhouse gas production and a diminution in the fertilizer, pesticide and water required for producing the associated crop feedstocks.

A method for producing a pyrolysis oil is described. In said method, a feedstock to be treated is first pyrolyzed in a pyrolysis zone, in which the feedstock is heated to a temperature of 250 degrees Celsius to 700 degrees Celsius; and pyrolyzed solids and pyrolysis vapors are formed. The pyrolysis vapors are then reformed at a temperature of 450 degrees Celsius to 1,200 degrees Celsius in a post-conditioning zone, in which the pyrolysis vapors are brought into contact with a catalyst bed, wherein the pyrolysis oil is formed. In this case, the catalyst comprises a pyrolyzed solid, which can be obtained according to the pyrolysis, described above. Finally the pyrolysis oil is separated from the additional pyrolysis products, which are formed, in a separation unit.

According to one embodiment, a system and process for the equalization of pressures of a flow stream across one or more valves is provided. A process circuit having clean non-abrasive fluid and at least one slave cylinder fbr transmitting pressure to a process flow stream is employed.

The present invention provides a spark ignition fuel mixture, comprising: a) diethyl ether with a content from 33.3 to 50 vol % of the mixture; b) ethanol with a content of at least 27 vol % of the mixture; and c) water with a content of at least 6 vol % of the mixture and not exceeding the ethanol content; wherein the mixture remains in a form of homogeneous liquid at −40° C. The present invention also provides a method of making or handling the spark ignition fuel mixture.

The present invention provides a spark ignition fuel mixture, comprising: a) diethyl ether with a content from 33.3 to 50 vol % of the mixture; b) ethanol with a content of at least 27 vol % of the mixture; and c) water with a content of at least 6 vol % of the mixture and not exceeding the ethanol content; wherein the mixture remains in a form of homogeneous liquid at −40° C. The present invention also provides a method of making or handling the spark ignition fuel mixture.

A high-gravity rotating bed device, including a motor, a rotor and a housing. The rotor and the motor are entirely arranged within the housing. A load-bearing plate is provided within the housing. The load-bearing plate divides the housing into a reaction chamber and a balance chamber. The motor is arranged within the balance chamber. A transmission shaft of the motor passes through the load-bearing plate and is fixedly connected to the rotor arranged within the reaction chamber. A gas inlet, a gas outlet, a liquid inlet and a liquid outlet are arranged on the housing. An externally communicating pipeline is arranged on the balance chamber. Also disclosed is an application of the present high-gravity rotating bed device under high-pressure conditions in operations such as mixing, transferring and reacting.