The present disclosure relates to polypeptides having alpha-glucosidase activity isolated, derived or derivable from Rasamsonia or engineered polypeptides having alpha-glucosidase activity isolated, derived or derivable from Rasamsonia homologs. The present disclosure also pertains to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, host cells and mutant cells comprising the polynucleotides. The disclosure further pertains to compositions comprising such polypeptides, methods of producing the polypeptides and compositions, as well as methods for using such polypeptides and compositions for industrial applications.

The invention relates to methods for preparing a beverage comprising at least 2 g/L -glucan, wherein said -glucans have an average molecular weight in the range of 80 to 200 kDa. The methods involve mashing barley kernels comprising at least 10% -glucans and having a ratio of DP3/DP4 in said -glucan of at least 3 in the presence of -amylase and endo-1,3(4)--glucanase activity. The beverages have a viscosity providing a good mouth-feel, and at the same time they comprise -glucans, which are able to aid in lowering LDL cholesterol levels. The beverages are generally stable and can be stored for months at room temperature without a significant decrease in -glucan content.

A method for producing a beer according to which a wort is prepared by brewing a mixture containing water and the malt of a cereal or a mixture of malts, then after cooling the wort, a yeast is added, selected from Saccharomyces cerevisiae, Saccharomyce carlsbergensis and mixtures thereof. After cooling of the wort, an enzyme is added, selected from the dextrinases and mixtures of dextrinases.

A method for producing a beer according to which a wort is prepared by brewing a mixture containing water and the malt of a cereal or a mixture of malts, then after cooling the wort, a yeast is added, selected from Saccharomyces cerevisiae, Saccharomyce carlsbergensis and mixtures thereof. After cooling of the wort, an enzyme is added, selected from the dextrinases and mixtures of dextrinases.

The present disclosure provides a composition for improving mouthfeel of beverages, comprising: a hydrolyzed beta-glucan characterized by an average molecular weight (M.sub.w) of about 50,000 to 350,000; and a hydrolyzed arabinoxylan, characterized by an average molecular weight (M.sub.w) of about 50,000 to about 350,000. Also provided are methods of improving the mouthfeel of a beverage product by adding to it a composition of the disclosure.

The present disclosure relates to polypeptides having alpha-glucosidase activity isolated, derived or derivable from Rasamsonia or engineered polypeptides having alpha-glucosidase activity isolated, derived or derivable from Rasamsonia homologs. The present disclosure also pertains to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, host cells and mutant cells comprising the polynucleotides. The disclosure further pertains to compositions comprising such polypeptides, methods of producing the polypeptides and compositions, as well as methods for using such polypeptides and compositions for industrial applications.

To provide an unfermented beer-taste beverage provided with fermentation flavor, and a method for producing the unfermented beer-taste beverage. An unfermented beer-taste beverage provided with good fermentation flavor characteristic of beer can be provided by a method for producing an unfermented beer-taste beverage comprising a step of adding a flavor composition containing a sulfur-containing compound to an unfermented beer-taste beverage to achieve a content of the sulfur-containing compound of 110.sup.2 ppt or more but 110.sup.3 ppt or less.

The present invention relates to isolated polypeptides having alpha-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

The present invention relates to isolated polypeptides having alpha-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

The present invention relates to polypeptides having glucoamylase activity with improved properties and to compositions comprising these polypeptides suitable for use in the production of a food, beverage (e.g. beer), feed, or biofuel. Also described is an improved and cost-effective process for isolating glucoamylases suitable for large scale protein purification procedures. Furthermore, different methods and uses related to glucoamylases according to the invention are disclosed, such as in a brewing process.