Fixed bed bioreactor with natural convection and forced draught to obtain bioactive substances by solid-state fermentation (SEF) using fungi macromycetes. This bioreactor may be from turn drum with pendulum motion and natural and forced convection to a tray bioreactor with natural convection. They are used in the production of bioactive substances as crude extracts of lignocellulosic enzymes and fungal polysaccharides obtained by using mixtures of lignocellulosic materials as substrates and macromycetes fungi as inoculum, controlling pH, humidity and particle size, inoculation rate and environmental conditions during fermentation such as temperature, relative humidity and carbon dioxide and oxygen concentration.

A method using a system adapted for continuous growth and harvesting of fungal fruiting bodies. The system includes a growth chamber, one or more mycelium feed assemblies, a nutrient reservoir with liquid media, one or more environmental controls to control an environment within the growth chamber and of the liquid media within the nutrient reservoir. One or more mycelium feed assemblies are arranged within the growth chamber. Each of the one or more mycelium feed assemblies includes a nutrient supply member and a mycelium colony which grows around the nutrient supply member, that continuously supplies liquid media to the mycelium colony. The method includes starting one or more mycelium colonies on the nutrient supply member, establishing that a mature colony has formed, and then maintaining the mature mycelium colony by continuous delivery of liquid medium to the mycelium colony, thus allowing for contiguous generation and harvesting of fungal fruiting bodies.

A method using a system adapted for continuous growth and harvesting of fungal fruiting bodies. The system includes a growth chamber, one or more mycelium feed assemblies, a nutrient reservoir with liquid media, one or more environmental controls to control an environment within the growth chamber and of the liquid media within the nutrient reservoir. One or more mycelium feed assemblies are arranged within the growth chamber. Each of the one or more mycelium feed assemblies includes a nutrient supply member and a mycelium colony which grows around the nutrient supply member, that continuously supplies liquid media to the mycelium colony. The method includes starting one or more mycelium colonies on the nutrient supply member, establishing that a mature colony has formed, and then maintaining the mature mycelium colony by continuous delivery of liquid medium to the mycelium colony, thus allowing for contiguous generation and harvesting of fungal fruiting bodies.

Apparatus 101 for producing a continuous stream of biohydrogen includes a continuous flow stirred tank reactor (CSTR) 12, lighting arrangement 13; ports 14; peristaltic pumps 16; and gas chromatograph (GC) 18. The CSTR 12 includes internal bioreactor chamber 20; a photosynthesizing microorganism inlet stream 22.1 for delivering Chlorella vulgaris; an organic waste inlet stream 22.2 for delivering wastewater activated sludge (WWAS); and a nutrient inlet stream 22.3 for delivering nutrients into the chamber 20; an outlet stream 24; and stirrers for stirring the contents of the chamber. The lighting 13 is provided by lamps 26 for transmitting light through a transparent sidewall of the chamber 20. The ports 14 and CG 18 are for taking measurements from the chamber 20. The pumps 16 control flowrate of the streams 22.1, 22.2, 22.3 and 24 for regulating biohydrogen production from fermentation while Chlorella vulgaris consumes oxygen to avoid biohydrogen conversion into methane.

Provided is a reaction apparatus for comprehensively measuring biodegradability of a material, comprising a device frame, an electrical control cabinet and a reaction chamber monomer. The upper side of the reaction chamber monomer is a reaction chamber body, and the lower part is a material receiving trolley. The top of the reaction chamber body is sealed by a chamber cover. A side wall is pasted with an electric heating plate and a thermal insulation cotton. A stirring paddle is arranged inside. An air inlet and an air outlet are respectively provided on a front and a rear wall. A discharging mechanism is located below. The electrical control cabinet separately controls the reaction conditions of each reaction chamber monomer. The present invention further relates to a use method thereof, which can realize the biodegradability evaluation in such three aspects as material degradation rate, disintegration rate and ecological non-toxicity test.

A system (100, 200, 400, 700) and method for growing biomass, wherein the system comprises at least one growing unit (110, 210, 410, 710) having a respective controllable environment and comprising a first area (120, 220, 420, 720) and a second area (130, 230, 430, 730). The system further comprises at least one plate-grid device (140, 240, 441, 442, 443, 500, 740) arranged within said at least one growing unit in turn comprising at least one plate (225, 325, 510) elevated from at least one grid (226, 326, 530, 631, 632, 633, 634) wherein the at least one plate and the at least one grid are independently movable from one another between the first area and the second area. Additionally, the system comprises a decontamination device (160, 260, 461, 462) arranged within the second area and configured to decontaminate, in the second area, one or more of the at least one plate and/or at least one grid of the at least one plate-grid device. Furthermore, the at least one plate and at least one grid of each at least one plate-grid device are movable independently from the at least one plate and at least one grid of the other plate grid devices between a loading position (171, 471, 771), at which the at least one plate is free to receive a growth medium for growing biomass, and a discharging position (172, 472, 772), within the first area. The system further comprises at least one transporting mechanism (150, 250, 450, 750) configured to transport grown biomass discharged from the at least one plate-grid device at the discharging position, to a third area arranged outside of the at least one growing unit.

The present disclosure relates to the technical field of biological treatment of exhaust gases, in particular to preparation of a complex microbial inoculant, an apparatus for preparing the complex microbial inoculant and application thereof. The preparation of the complex microbial inoculant is to select kitchen wastes, wood chips and EM fungus chaff as aerobic composting raw materials, and add contaminant degrading bacteria Pseudomonas mendocin NX-1, Stenotrophomonas sp. HY-2, Rhodococcus sp. YZ-1, Ralstonia sp. XZW-1, and Pseudomonas oleovorans DT4 during the aerobic composting process for joint culture until a compost pile becomes thoroughly decomposed. The complex microbial inoculant provided by the present disclosure can simultaneously degrade n-hexane, pentane, chlorobenzene and tetrahydrofuran, and has the remarkable advantages of simple preparation process, low cost, low carbon, environmental friendliness and the like. The formed microbial inoculant that is rich in efficient degrading bacteria does not need to adjust pH, has the advantages of short starting cycle, and stable and efficient exhaust gas purification, has stronger environmental adaptability, and lays a foundation for engineering application of purifying waste water and exhaust gases containing n-hexane, pentane, chlorobenzene and tetrahydrofuran by a biological method.

An extraction apparatus for a fermentation medium comprises an extraction chamber configured to receive a fermentation medium and comprising a lower portion and an upper portion above the lower portion, as well as a feed device configured to feed at least one gas and one liquid into the lower portion of the extraction chamber, as well as a discharge opening arranged in the upper portion of the extraction chamber and configured to drain a washout liquid, loaded with microorganisms, from the extraction chamber.

A method for the preparation of a mycelium colonized substrate includes a step of incubating a mycelium-inoculated substrate to grow the mycelium. The mycelium-inoculated substrate comprises a synthetic granulometry regulator. An intermediate product in the preparation of a mycelium colonized substrate includes mycelium colonized substrate and a synthetic granulometry regulator. A solid-state mycelium bioreactor is adapted for performing the method.

A synovium on a chip device having: a cartridge housing, a central chamber embedded in the cartridge housing, at least one aperture fluidly connected to the central chamber, and a plurality of evenly spaced micropillars arranged in a substantially circular shape within the central chamber such that the central chamber is partitioned into at least a first inner region and a first outer region, wherein the first inner region includes endothelial cells configured to mimic a vascular network and the first outer region includes fibroblast-like synoviocytes (FLS) configured to mimic a synovium.