Massively parallel high throughput single-cell optoperation and their uses thereof are disclosed. The disclosed method teaches the use of parallel single-cell printing technique using SU-8 membrane and nanosecond pulsed laser to espouse on single-cell with micro-dish pattern structure. The platform is able to effectively deliver different (small to large) cargo in a different cell type with high transfection efficiency and high cell viability at parallel single-cell resolution. The proposed Bio-MEMS device has potential applications in personalized and regenerative medicine.

A fluidic cartridge comprises a fluidic disk having a plurality of alignment openings; a fluidic chip comprising a body, one or more channels formed in the body in fluidic communications with input ports and output ports for transferring one or more fluids between the input ports and the output ports, and a plurality of protrusions formed on the body and received in the alignment openings of the fluidic disk for aligning the fluidic chip to the fluidic disk; an actuator operably engaging with the one or more channels for selectively and individually transferring the one or more fluids through the one or more channels from at least one of the input ports to at least one of the output ports at desired flow rates; and a tube member defining a cylindrical housing for accommodating the fluidic disk, the fluidic chip and the actuator therein.

The present invention relates to a device (100) for monitoring the development of a biological material, said device in the orientation intended for use comprising: a housing (2) having an extension in a longitudinal direction (X) and an extension in a transversal direction (Y), said housing comprises: two or more culture dish compartments (4), each adapted to accommodate a culture dish (6) comprising a biological material (8) to be monitored, each said compartment being separated from each of said other compartments, said two or more compartments being arranged along the longitudinal direction; each said culture dish compartment (4) comprising a lid (10) adapted to be able to be shifted between an open configuration in which access to the interior (12) of said culture dish compartment is provided, and a closed configuration sealing off the interior of said culture dish compartment from its surroundings; wherein each said culture dish compartment comprises an inlet (14) for supplying gas to and an outlet (16) for removing gas from said culture dish compartment; wherein each said culture dish compartment comprising heating means (18) for heating the interior of said culture dish compartment; wherein said housing comprises one or more cameras (20) for capturing images of a biological material in a culture dish.

The invention features devices and kits for capturing and culturing microorganisms (e.g., bacteria, fungi, or protists) and methods of using the devices and kits to detect microorganisms in environmental and other samples. The device includes a nutrient media having a flat growth area on which microorganisms can grow. Samples are collected by contacting the device with any environmental sample, e.g., rolling device on a work surface or exposing device to air, or by filtering a sample through a membrane. Microorganisms deposited on the membrane derive nutrients from the underlying media and grow into colonies that can then be detected using methods known in the art. The detected colonies can be imaged digitally or with film.

A culture dish combination for embryo thawing and embryo transfer is provided. The culture dish combination includes a first culture dish and a second culture dish, the first culture dish includes a first dish cavity for thawing and laser-assisted hatching of frozen embryos, and an opening of the first dish cavity is upward. The second culture dish is detachably connected to the first culture dish, and the second culture dish includes a second dish cavity for balancing culture medium before the embryo transfer; the second culture dish further includes a third dish cavity surrounding the second dish cavity, and openings of the second dish cavity and the third dish cavity are upward. The culture dish combination solves problems of complex operations of thawing the frozen embryo, laser-assisted hatching and the embryo transfer, which increases the accuracy of the operation of embryo thawing and improves the stability of the incubator environment.

Some systems, devices and methods detailed herein provide a restraint device for holding a microslide or another slide instrument in an operative position in a petri dish.

A continuous automated perfusion culture analysis system (CAPCAS) comprises one or more fluidic systems configured to operate large numbers of biodevices in parallel. Each fluidic system comprises an input reservoir plate for receiving media; a biodevice plate comprising an array of biodevices fluidically coupled to the input reservoir plate, configured such that each biodevice has independent media delivery, fluid removal, stirring, and gas control, and each biodevice is capable of continuously receiving the media from the input reservoir plate; and an output plate fluidically coupled to the biodevice plate for real-time analysis and sampling. The operations of the CAPCAS are automated and computer-controlled wirelessly. The CAPCAS can also be used for abiotic and biotic chemical synthesis processes.

When detachment of a cell sheet from a culture dish has started at an unintended timing, immediately detach the cell sheet from the culture dish while preventing generation of wrinkles and the like in the cell sheet. A cell culture device captures an image of cells in the culture dish, and controls a cooling mechanism and a shaking mechanism based on the image so as to shake the cells while cooling them.

When detachment of a cell sheet from a culture dish has started at an unintended timing, immediately detach the cell sheet from the culture dish while preventing generation of wrinkles and the like in the cell sheet. A cell culture device captures an image of cells in the culture dish, and controls a cooling mechanism and a shaking mechanism based on the image so as to shake the cells while cooling them.

A continuous automated perfusion culture analysis system (CAPCAS) comprises one or more fluidic systems configured to operate large numbers of biodevices in parallel. Each fluidic system comprises an input reservoir plate for receiving media; a biodevice plate comprising an array of biodevices fluidically coupled to the input reservoir plate, configured such that each biodevice has independent media delivery, fluid removal, stirring, and gas control, and each biodevice is capable of continuously receiving the media from the input reservoir plate; and an output plate fluidically coupled to the biodevice plate for real-time analysis and sampling. The operations of the CAPCAS are automated and computer-controlled wirelessly. The CAPCAS can also be used for abiotic and biotic chemical synthesis processes.