A multi-sensor component for the installation of at least two sensors at an individual port of a container for culturing biological material is provided. The multi-sensor component has a housing that can be introduced by a front housing segment into an uptake opening extending through the port of the container so that the front housing segment is facing the inside of the container. The multi-sensor component has a first sensor unit or a mount for a first sensor unit arranged on the front housing segment and has a second sensor unit or a mount for a second sensor unit arranged on the front housing segment.

A cell culture vessel, cell culture monitoring system, and Raman spectroscopy system for non-invasive measuring of a cell culture are provided. The vessel includes a cell culture chamber that operates as a closed system, a wall defining a boundary of the cell culture chamber and separating the interior space of the cell culture chamber from an exterior of the cell culture chamber, and a window disposed in the wall and separating the interior space of the cell culture chamber from an exterior of the cell culture chamber. The cell culture chamber includes an interior space for housing at least one of the cell culture and a cell culture media. The window includes a polymer and allows monitoring of the cell culture via a monitoring module disposed on the exterior without the monitoring module coming into physical contact with the cell culture or the cell culture media.

A cell cultivation apparatus for cultivating microorganisms and growing cells at high density is provided. The apparatus includes a membrane comprising multiple surface features on a first side of the membrane for cell placement. The surface features comprising one or more compartments within which a cell can be located. The membrane includes a material that is at least partially permeable to gas. A second side of the membrane defines a gas region. The second side of the membrane is separated from the first side of the membrane by the membrane. The apparatus further includes a media region for receiving media. The compartments are configured to at least partially reduce media flow shear forces on one or more cells in the compartments. The surface features may be ridges, protrusions, fins, wells, and/or posts.

A cell culture vessel (100) has walls and a substrate having a plurality of microcavities (120), where each microcavity of the plurality of microcavities includes a concave well and an opening to allow the microcavity to be filled with liquid. A flange (170) surrounds the substrate having an array of microcavities. A channel (175, 176) surrounds the flange, providing a moat around the microcavity substrate. The flange is angled. Methods of culturing cells in the cell culture vessel are also provided.

Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.

A full-ocean-depth fidelity enzymological measurement device for microbial extracellular enzyme is provided, comprising a pressure-maintaining sampling bottle, pressure-maintaining transfer equipment, a pressure-maintaining enzymological reactor, and heat preservation equipment and enzyme activity detection equipment. The pressure-maintaining enzymological reactor comprises a barrel body, a plug, polytetrafluoroethylene gaskets, an O-ring, a piston, a high-pressure straight-through valve, and a high-pressure connector. The pressure-maintaining enzymological reactor is in a closed barrel body shape and is internally provided with the piston, and the plug and the valve are arranged at each of two ends of the pressure-maintaining enzymological reactor; and the valve is connected to the pressure-maintaining transfer equipment through the high-pressure connector. According to the full-ocean-depth fidelity enzymological measurement device provided by the present disclosure, full-ocean-depth (0-11000 m) sample pressure-maintaining sampling and transferring can be achieved; and sample collection, transferring and enzymological reaction can be conducted under in-situ pressure and temperature conditions.

Improved cell culture devices and related methods that overcome the limitations of prior devices and methods, by creating devices that can integrate a variety of novel attributes. These various attributes include the use of gas permeable material and medium volumes that exceed conventional devices as well as compartments that can facilitate the long term study of high density cultures with reduced disruption of the culture environment, the ability to study the migration of items of interest including substances such as chemokine, track the movement of cells, and monitor cell to cell interactions.

Provided is a culture chamber that includes a plurality of recesses each formed of a bottom portion and an opening portion. The bottom portion has a hemispherical shape or a truncated cone shape. The opening portion is defined by a wall that surrounds an area from a boundary between the opening portion and the bottom portion to an end of each of the recesses, the wall having a taper angle in a range from 1 to 20 degrees. An equivalent diameter of the boundary is from 50 μm to 2 mm and a depth from a bottom of the bottom portion to the end of each of the recesses is from 0.6 or more times to 3 or less times the equivalent diameter, and the wall defining the opening portion forms a surface continuous to the bottom portion. An inclination of the continuous surface changes at the boundary.

Disclosed herein is a sterilizable adapter for a cell culture vessel comprising a body, an upper connection and/or a lid, at least one port, a ventilation passage, a lower internal connection suitable for connection to a cell culture vessel and a cell retention device and/or a cell separation device as well as a device comprising said sterilizable adapter and a method for cell cultivation using said sterilizable adapter.

A fluidic cartridge comprises a fluidic disk having a plurality of alignment openings; a fluidic chip comprising a body, one or more channels formed in the body in fluidic communications with input ports and output ports for transferring one or more fluids between the input ports and the output ports, and a plurality of protrusions formed on the body and received in the alignment openings of the fluidic disk for aligning the fluidic chip to the fluidic disk; an actuator operably engaging with the one or more channels for selectively and individually transferring the one or more fluids through the one or more channels from at least one of the input ports to at least one of the output ports at desired flow rates; and a tube member defining a cylindrical housing for accommodating the fluidic disk, the fluidic chip and the actuator therein.