C12M1/26

Microfluidic chip, three-dimensional channel structure, cell culture method using same, and activity evaluation method of bioactive substance using same

The microfluidic chip according to an embodiment of the present invention may include a plate, a bridge channel formed in intaglio on one side of the plate, an inlet formed through the plate to communicate with one end of the bridge channel, an outlet formed through the plate to communicate with the other end of the bridge channel, and at least one well extending in an outward direction of the plate from the bridge channel to provide a space, wherein the bridge channel may be in the form of a curved line, a bent line, an arc, a circle, a spiral, or a polygon.

Methods, devices, and apparatus for washing samples on array plates

An apparatus for washing an array plate includes one or more dispensers and one or more aspirators that are distinct from the one or more dispensers. A respective dispenser of the one or more dispensers is configured to dispense a first liquid on the array plate, and a respective aspirator of the one or more aspirators is configured to aspirate liquid on the array plate. A method for washing particles is also disclosed.

Micro algae harvesting methods and devices

The present invention relates to a particulate material separation assembly. It comprises a filtration membrane and an antifouling device. The antifouling device comprises one or more magnets and a plurality of magnetisable particles. The one or more magnets cause the plurality of magnetisable particles to self-assemble into dynamic bristles, thereby forming a brush. The particulate material separation assembly is particularly useful in the context of micro algae harvesting.

Systems for utilizing crossflow filtration for cell enrichment

A disposable cell enrichment kit includes a crossflow filtration device configured to be disposed along a main loop pathway and to receive a process volume containing a biological sample and utilize crossflow filtration, via a micro-porous membrane, to retain a specific cell population in a retentate from the process volume and to remove a permeate including certain biological components from the process volume. The crossflow filtration device includes a laminated filtration unit that includes the micro-porous membrane, a first mating portion, a second mating portion, and a membrane support. The membrane support includes a first plurality of structural features that define a first plurality of openings, wherein the first plurality of structural features are coupled to the micro-porous membrane and provide support to the micro-porous membrane, and the first plurality of openings allow the permeate to flow through them after crossing the micro-porous membrane.

Bio sample collection device
11543330 · 2023-01-03 · ·

A bio sample collection device including: a housing; a cap part detachably fastened to the housing, and including a bio sample collector for collecting a bio sample; and a bio sample processing liquid storage unit storing bio sample processing liquid, the bio sample processing liquid storage unit being formed to be broken when the cap part is combined with the housing after bio sample is collected so that the bio sample processing liquid is mixed with the bio sample.

Safety cabinet

An object of the present invention is to provide a safety cabinet capable of controlling the purity during usage. In order to realize the object, the safety cabinet is configured to include an operation space including an operation stage; a front panel formed in a front surface of the operation space; an operation opening provided in a lower portion of the front panel; a suction port that is provided in the vicinity of the operation opening on a front side of the operation stage to lead downward; an air circulation path through which air suctioned from the suction port flows along a lower portion, a back surface, and an upper portion of the operation space; a particle counter; an operation space-air intake port provided in the operation space to take air into the particle counter; and an introduction pipe that introduces the air into the particle counter from the operation space-air intake port.

FIXED BED CELL CULTURE AND HARVESTING SYSTEM AND METHODS OF USING THE SAME

A cell culture system is provided that includes a cell culture vessel having an interior cavity to house a cell culture substrate in a cell culture space, and at least one port for at least one of fluid inlet to the interior cavity and fluid outlet from the interior cavity. The system further includes a piston having a distal end disposed in the cell culture vessel above the cell culture space, the distal end of the piston being sealed with an airtight seal within the interior cavity. The system also includes a driver coupled to the piston to move the piston so as to increase and decrease a distance between the distal end and the cell culture space. The driver can pressurize the interior cavity via actuation of the piston to harvest cells from the cell culture space through the at least one port.

System and method for transferring tissue

A transfer device designed to extract an amorphous or semi-solid structure, tissue, or construct from supporting media while maintaining the spatial integrity/organizational architecture thereof. The transfer device can include a controller, an actuator assembly, a plunger, and a needle. The controller can move the transfer device and the plunger independently.

CELL TRANSPLANTATION PRETREATMENT METHOD, CELL TRANSPLANTATION PRETREATMENT DEVICE, AND CELL TRANSPLANTATION PRETREATMENT UNIT
20220396755 · 2022-12-15 · ·

A cell transplantation pretreatment method, a cell transplantation pretreatment device, and a cell transplantation pretreatment unit are provided, which are capable of improving the efficiency of loading grafts into a transplantation device. The cell transplantation pretreatment method includes the steps of: transporting a cell group cultured in a culture recess to a pretreatment recess using a culture tray including a plurality of the culture recesses arranged according to a first arrangement, a pretreatment tray including a plurality of the pretreatment recesses arranged according to a second arrangement, and a transplantation device including a needle shaped portion having a tubular shape configured to allow entry and exit of a graft containing the cell group, the transplantation device including a plurality of the needle shaped portions arranged according to the second arrangement; and loading the cell groups simultaneously from the plurality of pretreatment recesses into the plurality of needle shaped portions.

METHOD AND DEVICE FOR THE PRODUCTION OF MICROALGAE BIOMASS
20220389357 · 2022-12-08 ·

The invention relates to the production of microalgae biomass. The microalgae contained in a suspension of water and microalgae are continuously phototrophically or mixotrophically cultivated in a cultivation module (1), which is passed multiple times by the suspension and has a gas part and a liquid part with a liquid supply (3), by supplying light from at least one artificial light source (5) and nutrients. According to the turbidity established by sensors, volume fractions of the suspension are repeatedly discharged from the cultivation module (1) for the harvest of microalgae and removed by means of a centrifuge (7). The cultivation of the microalgae occurs in an climate chamber forming the cultivation module (1), which is operated using water. Alongside a regulating of the temperature of the suspension, there also occurs a regulating of its pH value via the controlled addition of buffer ions and a regulating of the redox potential of the suspension and thereby also of its microbial contamination by controlling the light and nutrient supply, as well of a metered addition of oxygen. In addition, after the removal of microalgae, the remaining suspension is irradiated with UV light in order to kill unwanted microbial contamination before being returned into the cultivation module (1).