A method for processing biological material containing stringy tissue in a container having a tissue collector disposed in a tissue retention volume on one side of an internal filter includes washing biological material contained in the tissue retention volume with wash liquid to the tissue retention volume and allowing the wash liquid and rotating the tissue collector disposed in the tissue retention volume relative to the container in a first direction of rotation about an axis of rotation to sweep the teeth positioned on the tissue collector through the biological material and to collect stringy material on the tissue collector.

Moisturized organic material is treated by communicating with a vacuum pump (44) to selectively reduce pressure within a vessel (12) below atmospheric pressure and to a vaporization point, such as at 30 to 17.8° C., for a period of time sufficient to create an internal pressure to rupture cell walls. While being subjected to vacuum below atmospheric pressure, the vessel (12) is rotated to homogenize the moisturized organic material. The organic material can be moisturized by non-potable water including moisture collected by a moisture collector (44b) in the conduit (44a) between the vessel (12) and the vacuum pump (44) and by chilled feed water acting as the seal fluid in the vacuum pump and heated while flowing through a heat exchanger (148) of the moisture collector (44b). Heat can be added by a heater (46) to raise the temperature of low ambient temperature moisturized organic material.

The present invention concerns a single use aseptic kit comprising: a disaggregation module for receipt and processing of material comprising solid mammalian tissue; and a stabilisation module for storing disaggregated product material, wherein each of said modules comprises one or more flexible containers connected by one or more conduits adapted to enable flow of the tissue material there between; and wherein each of said modules comprises one or more ports to permit aseptic input of media and/or reagents into the one or more flexible containers. The invention further relates to an automated device for semi-automated aseptic disaggregation and/or enrichment and/or stabilisation of cells or cell aggregates from mammalian solid tissue comprising a programmable processor and the single use aseptic kit. The invention further relates to a semi-automatic aseptic tissue processing method.

An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process), with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.

Anaerobic digestion apparatus comprises a first chamber for retaining organic matter before and/or during anaerobic digestion and a second chamber for retaining organic matter during anaerobic digestion. The anaerobic digestion apparatus is configured to refrigerate or heat the first chamber to suppress methanogenesis in the first chamber. The anaerobic digestion apparatus comprises a controller programmed to regulate the anaerobic digestion process and to thereby reduce system perturbations. The flow of organic matter to the second chamber where methanogenesis is regulated. There is disclosed an inoculum for anaerobic digestion comprising Acetobacterium woodii and Methanosaeta concilii.

A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.

A bead beater homogenizer includes a shaft having a main body extending along a main axis and a distal connection body extending along a connection axis that is acutely angled with respect to the main axis, a motor configured to rotate the shaft about the main axis, a head rotatably connected to the distal connection body of the shaft, and a clamp secured to the head and configured to secure a sample vial holder configured to hold one or more sample vials therein, wherein rotational motion of the shaft about the main axis is translated into motion of the head in directions normal to the main axis. A sample vial holder having an internal network of channels defined within the housing through which a coolant can be passed to control a temperature of a vial disposed therein is also provided.

A container is disclosed for isolating living cells. The container for crushing a sample by pressing includes a container main body having an opening part and a partition which separates an upper space and a lower space of the container main body, in which at least a part of the partition has a portion which is not horizontal with respect to the bottom of the container.

A method of preparing adipose tissue for transplantation, from lobular fat extracted, for instance, by liposuction, the fat including a fluid component that also includes an oily component, a blood component and/or sterile solutions and of a solid component that includes cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, characterized in that it includes at least one step of forming an emulsion of the fluid component in the sterile washing solution by active and/or passive stirring and discharging the emulsion from the outlet through a density gradient.

Anaerobic digestion apparatus comprises a first chamber for retaining organic matter before and/or during anaerobic digestion and a second chamber for retaining organic matter during anaerobic digestion. The anaerobic digestion apparatus is configured to refrigerate or heat the first chamber to suppress methanogenesis in the first chamber. The anaerobic digestion apparatus comprises a controller programmed to regulate the anaerobic digestion process and to thereby reduce system perturbations. The flow of organic matter to the second chamber where methanogenesis is regulated. There is disclosed an inoculum for anaerobic digestion comprising Acetobacterium woodii and Methanosaeta concilii.