The invention relates to a method for culturing and detecting microorganisms, comprising the steps of providing a liquid sample (S) in a barrel (10) of a device (1) for culturing and detecting microorganisms, passing the liquid sample (S) through a first filtering membrane (40) such that microorganisms contained in the liquid sample (S) are retained at a first side (41) of the first filtering membrane (40), contacting said first side (41) with a first growth medium (210) capable of supporting growth of microorganisms, incubating the first filtering membrane (40) and the first growth medium (210) at an incubation temperature, arranging a sensing surface (51) of a gas sensor (50) in fluid connection with a second side (42) of the first filtering membrane (40), detecting a metabolic gas released by the microorganisms by means of the gas sensor (50). The invention further relates to a device (1) for culturing and detecting microorganisms, comprising a barrel (10) enclosing a barrel compartment (13) for receiving a liquid sample (S), a first piston (20) which (20) is movable in said barrel (10), wherein said barrel compartment (13) is configured to be brought in fluid communication via a first filtering membrane (40) with a sensing surface (51) of a gas sensor (50) configured to detect a metabolic gas released by microorganisms, wherein the first filtering membrane (40) is configured to retain microorganisms contained in the liquid sample (S) at the first side (41) of the first filtering membrane (40). Furthermore, a sensor cartridge (4) and a kit of parts comprising the device (1) are provided.

In various embodiments a culture platform for cultivating tissue is provided, comprising: a first component (2) comprising at least one culture chamber (21) formed therein, the culture chamber (21) comprising a bottom (23), a sidewall and an open top (22); and a second component (2) comprising a base (31) and at least one pair of posts (32) extending from the base (31); wherein the first component (2) and the second component (3) are sized and configured to be mated with one another such that when the second component (3) is placed on the first component (2), the at least one pair of posts (32) is inserted into the at least one culture chamber (21). Furthermore, a method for observing tissue cultivated therein is provided.

In a first aspect, the present invention relates to a method of producing a library of microorganisms, the method comprising the steps of: a. providing a first fluid comprising at least one single cell, b. dispersing said first fluid comprising at least one single cell in a second fluid, thereby obtaining a plurality of single-layer microfluidic droplets, wherein at least one single- layer microfluidic droplet comprises at least one single cell, wherein the second fluid is immiscible with the first fluid, c. optionally, adding to said at least one single-layer microfluidic droplet a third fluid comprising a sensing compound, wherein the third fluid is miscible with said first fluid, and wherein the third fluid is immiscible with said second fluid, d. injecting said at least one single-layer microfluidic droplet optionally comprising the sensing compound into a fourth fluid, wherein said fourth fluid is immiscible with said second fluid, thereby obtaining at least one double-layer microfluidic droplet, e. dispensing said at least one double-layer microfluidic droplet into a culture medium based on the viability of the cell, f. incubating said culture medium, thereby obtaining said library. In a second aspect, the present invention relates to a system comprising: a. a first microfluidic chip for producing a plurality of single-layer microfluidic droplets wherein at least one single-layer microfluidic droplet comprises at least one single cell, b. a first microfluidic device for collecting said plurality of single-layer microfluidic droplets, c. a second device for adding a sensing compound into said at least one single-layer microfluidic droplet comprising at least one single cell, d. a second microfluidic chip for producing a double-layer microfluidic droplet, and e. a dispensing unit. In a third aspect, the present invention relates to the use of the method according to the first aspect of the present invention in a system according to the second aspect of the present invention.

A cell culture apparatus includes: a substrate having a first surface; a pair of structures each having a wall surface intersecting the first surface, the wall surfaces facing each other; and an electrode disposed on the first surface and traversing a space between the wall surfaces, the electrode and each of the wail surfaces forming an angle other than 90 degrees.

A reversible liquid filtration system for cell culture perfusion comprises: a bioreactor vessel (B4), for storing the cell culture (L4); a perfusion pump (P7), comprising a reciprocable element (P71) which is movable in opposing first and second pumping directions (dF, dR); a filter (F4); and first and second bi-directional valves (BV1, BV2), each selectively controllable between open and closed positions. The perfusion pump (P7), the filter (F4), and the first and second bi-directional valves (BV1, BV2), together comprise a fluidic circuit in communication with the bioreactor vessel (B4). The bi-directional valves (BV1, BV2) are controllable to open and close in co-ordination with the reciprocating perfusion pump (P7), in order to enable both a two-way filtering flow around the fluidic circuit and also an alternating filtering flow between the bioreactor vessel (B4) and the perfusion pump (P7).

A test container includes an inlet, a first storage portion, a second storage portion, a first flow channel that connects the first storage portion to the second storage portion, a first cylinder of which one end is connected to the first storage portion via a second flow channel and the other end is open to an outside, a second cylinder of which one end is connected to the second storage portion via a third flow channel and the other end is open to an outside, a first plug provided in the first cylinder, and a second plug provided in the second cylinder. An internal space including the first storage portion, the second storage portion, the first flow channel, the second flow channel, and the third flow channel is capable of being pressurized in a case where the first plug and the second plug are pressed and moved from the outside.

The purpose of the present invention is to collect a plurality of microscopic objects dispersed in a liquid by light irradiation, and also trap them. A collecting device for bacteria collects a plurality of bacteria dispersed in a sample liquid. The collecting device is provided with a laser beam source that emits laser beam and a honeycomb polymer film constituted so as to be able to hold the liquid. Walls prescribing pores for trapping the plurality of bacteria dispersed in the liquid are formed on the honeycomb polymer film, and also a thin film that includes a material for converting light from the laser beam source to heat is formed on the honeycomb polymer film. The thin film heats the liquid of the sample through the conversion of the laser beam from the laser beam source to heat, thereby causing a convection in the liquid.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

A bioprocess system and a method for incubating, growing and harvesting cell cultures is described. Also disclosed is a bioprocess container that can be used with the system. In one aspect of the present disclosure, the bioprocess system includes bioprocess tubes and cell culture tubes having particular dimensions and being made from specific materials that allow the tubes to be welded together while preventing open connections and/or ruptures. In this manner, bioprocess containers can be connected and disconnected from a cell culture apparatus without having to perform the manipulation within a closed environment and without associated monitoring.

The present disclosure relates to methods and apparatus for measuring of multiple physiological properties of biological samples, such as measuring biological flux.