In a method for cultivating biological cells of differing types, carriers are stored in an incubator, in which the carriers comprise one or more storage chambers. One or more cultures comprising cells of a common type are stored in one of the storage chambers. Cultivation parameters are assigned to the cultures. Datasets containing organizational coordinates of each storage chamber and the cultivation parameters of the culture stored therein are stored in a data processing device. The carriers are removed from the incubator at predefined time intervals in order to treat the cultures according to their respective cultivation parameters. The datasets are divided into groups with correlated cultivation parameters. The cultures are subsequently treated in groups.

Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.

A system includes a bioprocessing vessel formed of a material insulative to a liquid medium when present therein. At least one sensor is for sensing a parameter of the bioprocessing vessel and generating a signal indicative of the parameter. At least ne conductor is adapted to provide electrical communication between the liquid medium in the bioprocessing vessel and an external structure to achieve equipotential for reducing noise in the signal produced by the at least one sensor. Related apparatus and methods are also disclosed.

A reversible liquid filtration system for cell culture perfusion comprises: a bioreactor vessel (B4), for storing the cell culture (L4); a perfusion pump (P7), comprising a reciprocable element (P71) which is movable in opposing first and second pumping directions (dF, dR); a filter (F4); and first and second bi-directional valves (BV1, BV2), each selectively controllable between open and closed positions. The perfusion pump (P7), the filter (F4), and the first and second bi-directional valves (BV1, BV2), together comprise a fluidic circuit in communication with the bioreactor vessel (B4). The bi-directional valves (BV1, BV2) are controllable to open and close in co-ordination with the reciprocating perfusion pump (P7), in order to enable both a two-way filtering flow around the fluidic circuit and also an alternating filtering flow between the bioreactor vessel (B4) and the perfusion pump (P7).

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

The present invention is intended to provide a cell treatment apparatus and a method for treating cells that can suppress a variation of a treatment time of treating cells using laser light. The cell treatment apparatus of the present invention includes: a cell treatment chamber in which cells in a cell culture vessel are treated; an observation unit that can observe the cells; a laser projection unit that can project a laser image onto the cells; a laser moving unit that can move the laser projection unit; and a control unit. The laser projection unit includes: a laser light source; and a laser image generation portion that generates the laser image to be projected onto the cells from laser light oscillated from the laser light source. The control unit controls generation of the laser image by the laser image generation portion. By moving the laser moving unit from a projection start position of the laser image at one end of the cell culture vessel to a projection end position of the laser image at the other end of the cell culture vessel, the laser projection unit projects the laser image from the projection start position of the laser image at one end of the cell culture vessel to the projection end position of the laser image at the other end of the cell culture vessel.

A system and method for automated testing a sample of a body fluid for the presence of bacteria is described. The system includes a fluid handling device, Incubator, flow cytometer, at least a processor, and a memory configuring the at least a processor to distribute a portion of the plurality of fluid samples within a well plate to at least a first well, divide the portion of the plurality of fluid samples from the at least a first well into at least two wells including a T.sub.0 well and a T.sub.1 well, obtain a T.sub.0 enumerative baseline bacterial value at time T.sub.0, culture the fluid samples in the T.sub.1 well using the incubator, obtain a T.sub.1 enumerative control bacterial value at time T.sub.1, and determine a presence of bacteria as a function of the T.sub.0 enumerative baseline bacterial value and the T.sub.1 enumerative control bacterial value.

A system and method for automated testing a sample of a body fluid for the presence of bacteria is described. The system includes a fluid handling device, Incubator, flow cytometer, at least a processor, and a memory configuring the at least a processor to distribute a portion of the plurality of fluid samples within a well plate to at least a first well, divide the portion of the plurality of fluid samples from the at least a first well into at least two wells including a T.sub.0 well and a T.sub.1 well, obtain a T.sub.0 enumerative baseline bacterial value at time T.sub.0, culture the fluid samples in the T.sub.1 well using the incubator, obtain a T.sub.1 enumerative control bacterial value at time T.sub.1, and determine a presence of bacteria as a function of the T.sub.0 enumerative baseline bacterial value and the T.sub.1 enumerative control bacterial value.

A system and method for regression modeling an interior volume of a containment vessel and interpolating data from multi-point sensor arrays within the containment vessel to detect conditions across the interior volume of the containment vessel.

A method for generating a maintenance program for the operation of a maintenance system at a bioreactor, in particular a bioreactor of a vehicle for transporting persons, which method comprises at least the following steps, which are executed by an electronic data processing means associated with the maintenance system: acquiring system characteristics data of the maintenance system; acquiring reactor characteristics data of the bioreactor, the reactor characteristics data being received at least in part from a communication interface of the bioreactor; and generating the maintenance program at least on the basis of the system characteristics data and the reactor characteristics data.