A portable fluidic platform for rapid and flexible end-to-end production of recombinant protein biologics includes a bioreactor system hosting stable and robust cell-free translation systems that is fluidically integrated with modular protein separation functionalities (e.g., size exclusion, ion exchange or affinity chromatography systems) for purification of the cell-free expressed product and which are configurable for process-specific isolation of different proteins, as well as for formulation. The bioreactor utilizes lysates from engineered eukaryotic (e.g., yeast) or prokaryotic (e.g., bacterial) strains that contain factors for protein folding and posttranslational modifications. Combination of various purification modules on the same fluidic platform allows flexibility of re-routing for purification of different proteins depending on specific target requirements. Protein synthesis and purification modules are integrated into self-contained disposable fluidic cartridge that eliminates cross-contamination between runs. The platform allows for flexible production of protein biologics within 24 hours (from DNA to purified product).

A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.

A production equipment of phenethylamine includes a first reaction device; a second reaction device, wherein the second reaction device is connected to the first reaction device; a circulation device, wherein the circulation device is respectively connected to the first reaction device and the second reaction device; an acetone storage device; a centrifugal extraction device; a phenethylamine processing device; and a phenethylamine storage device. The first reaction device and the second reaction device have the same structure. A production method of phenethylamine is further provided, which uses transaminase as a catalyst, so that acetophenone and isopropylamine can flow through the transaminase to complete the reaction, the reaction is completed in one step, the production cycle is shortened, and the production cost is reduced.

Provided herein are a device and a method for preparation of immobilized proteins, enzymes or cells on a carrier to achieve the industrial batch production of the immobilized proteins, enzymes or cells.

In the combined process for several biorefinery products obtained from a UMC (Undefined Mixed Culture) type of reaction it is possible to obtain biochemicals, energy gases, soil improvement etc. from a MPBU (Multipurpose Biorefinery Unit). The economically beneficial as well as environmentally sustainable results of the arrangement are demonstrated by the integrated process using two reactor systems with zero fiber for the production of lactate (in both the reactors pools 1 and 2). Additionally, mannitol can be produced in one of the reactor pools (number 2). It is possible to a. combine the processes taking into account their biochemical characteristics, b. produce gaseous substances for energy and industrial use, c. obtain organic fertilizers which can be microbiologically upgraded d. improve the adjustability for optimization of the various partial reactivities.

The chemical production occurs in two pools which advantageously are inoculated simultaneously.

The present invention relates to a bioreactor for the catalytic conversion of a substrate to a product using an immobilized enzyme. The immobilized enzyme is a histidine tagged enzyme, which binds to a nickel-nanoparticle coated cellulose matrix which is housed within the bioreactor. The invention also relates to methods of producing products by enzymatic catalysis using the bioreactor of the invention.

Aspects of the invention include methods of removing carbon dioxide (CO.sub.2) from a CO.sub.2 containing gas. In some instances, the methods include contacting CO.sub.2 containing gas with a bicarbonate buffered aqueous medium under conditions sufficient to produce a bicarbonate rich product. Where desired, the resultant bicarbonate rich product or a component thereof may then be stored or further processed, e.g., combined with a divalent alkaline earth metal cation, under conditions sufficient to produce a solid carbonate composition. Aspects of the invention further include systems for practicing the methods, as well as products produced by the methods.

An immobilized enzymatic reactor can include a wall defining a chamber having an inlet and an outlet; a solid stationary phase covalently linked to an enzyme and disposed within the chamber; and a pressure modulator in fluid communication with the chamber and adapted to support continuous flow of a liquid sample comprising a polymer analyte through the inlet, over the solid stationary phase, and out of the outlet under a pressure between about 2,500 and 35,000 psi. In one example, the solid stationary phase includes inorganic/organic hybrid particles in an ultra performance liquid chromatography system, the enzyme is a protease, and the polymer analyte is a polypeptide. The immobilized enzymatic reactor can prepare an analyte for applications such as for hydrogen deuterium exchange mass spectrometry.

An embodiment of the present disclosure provides an apparatus for immobilizing a microbial cell, the apparatus including: a mixing tank in which a cell-carrier-containing mixed solution is accommodated; a nozzle part through which the cell-carrier-containing mixed solution is injected from the mixing tank and is discharged to the outside; and a reaction tank in which a cell immobilized bead is formed by contact between the cell-carrier-containing mixed solution discharged from the nozzle part and an aqueous curing agent solution. In the apparatus for immobilizing a microbial cell according to the present disclosure, since the cell-carrier-containing mixed solution is injected through an air spraying nozzle, even when an immobilized carrier solution having a high viscosity is used, a microbial cell immobilized bead having a small size and having a spherical shape, or an almost spherical shape may be mass-produced.

A container set including: a filter unit; and a closing unit that is attachable to and detachable from the filter unit. The filter unit includes: a housing having a sample introduction opening provided at one end, and a liquid discharging opening provided at the second end; and a filter fixed to the housing. The housing has a first joining part, and the closing unit has a second joining part configured to be joinable with the first joining part. The first joining part and the second joining part are configured such that the liquid discharging opening is spatially closed in a state in which the two joining parts are joined together. A space between the closing unit and the filter is made into a positive pressure relative to a pressure in the sample holding space by pushing the filter unit and the closing unit such that the distance therebetween is shortened.