The present invention provides a cell culture apparatus. A cell culture apparatus according to one embodiment of the present invention is a cell culture apparatus for culturing a cell to form the cell into a three-dimensional spheroid. The cell culture apparatus may comprise: a plate; and a well which is arranged, in plural, on one side of the plate at predetermined intervals, has cells cultured inside, and has a micropattern disposed on the inner surface such that the inner surface and the cells are spaced apart from each other.

The present invention provides a culture vessel and a measuring device for measuring a cell sheet thickness nondestructively during culturing. A culture vessel having a lid 4 has a float 8 installed in the interior and the float is structured so as to be uplifted by a culture medium in the culture vessel. Further, a cell sheet thickness measuring device having a sensor head 10 having a light-emitting element 10A and a light-receiving element 10B to detect a positional variation of the float in the vertical direction and a controller 11 to process a signal of the sensor head is configured.

The present invention provides a culture vessel and a measuring device for measuring a cell sheet thickness nondestructively during culturing. A culture vessel having a lid 4 has a float 8 installed in the interior and the float is structured so as to be uplifted by a culture medium in the culture vessel. Further, a cell sheet thickness measuring device having a sensor head 10 having a light-emitting element 10A and a light-receiving element 10B to detect a positional variation of the float in the vertical direction and a controller 11 to process a signal of the sensor head is configured.

Disclosed is a bioreactor including a rotation device that rotates a reaction container while a reference direction is taken as an axial direction thereof, and an angle adjusting device coupled to the rotation device to adjust an angle defined by the reference direction, which is dependent on an arrangement posture of the rotation device, with respect to a ground surface, by changing the arrangement posture of the rotation device.

Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.

A cell culture apparatus includes one or more plates having a first major surface and an opposing second major surface. The first major surface comprises a structured surface defining a plurality of wells. Each well has an interior surface defining an upper aperture and a nadir, wherein the upper aperture of each well has a diametric dimension in a range from 100 micrometers to 2000 micrometers. The apparatus also includes a plurality of spacers extending from the first major surface along a length of the bottom surface. A plurality of flow channels are defined between adjacent rails.

A cell culture apparatus includes one or more plates having a first major surface and an opposing second major surface. The first major surface comprises a structured surface defining a plurality of wells. Each well has an interior surface defining an upper aperture and a nadir, wherein the upper aperture of each well has a diametric dimension in a range from 100 micrometers to 2000 micrometers. The apparatus also includes a plurality of spacers extending from the first major surface along a length of the bottom surface. A plurality of flow channels are defined between adjacent rails.

Disclosed herein are systems, devices, and methods for sterilization. In some variations, a method of sterilizing a bioreactor system may comprise circulating a sterilant in a bioreactor and an enclosed vessel in fluidic communication with the bioreactor. The enclosed vessel may comprise cell culture media. The sterilant may be circulated for a dwell time sufficient to sterilize at least one of the bioreactor, the vessel, and the cell culture media.

A cell culture apparatus includes one or more plates having a first major surface and an opposing second major surface. The first major surface comprises a structured surface defining a plurality of wells. Each well has an interior surface defining an upper aperture and a nadir, wherein the upper aperture of each well has a diametric dimension in a range from 100 micrometers to 2000 micrometers. The apparatus also includes a plurality of spacers extending from the first major surface along a length of the bottom surface. A plurality of flow channels are defined between adjacent rails.

A cell culture apparatus includes one or more plates having a first major surface and an opposing second major surface. The first major surface comprises a structured surface defining a plurality of wells. Each well has an interior surface defining an upper aperture and a nadir, wherein the upper aperture of each well has a diametric dimension in a range from 100 micrometers to 2000 micrometers. The apparatus also includes a plurality of spacers extending from the first major surface along a length of the bottom surface. A plurality of flow channels are defined between adjacent rails.