The invention relates to a method for the simultaneous production of a solid transformation product of the substrate and crude ethanol comprising the following steps: •preparing a substrate from milled or flaked biomass comprising proteinaceous matter which originates from soya bean, rape seed, or mixtures thereof, optionally in further mixture with proteinaceous matter originating from fava beans, peas, sunflower seeds, lupine, cereals, and/or grasses, •mixing said substrate with live yeast in a dry matter ratio of from 1:1 to 10,000:1 and adding water in an amount which provides a ratio of wet bulk density to dry bulk density from 0.60 to 1.45 in the resulting mixture; •incubating said mixture for 1-48 hours at a temperature of about 20-60° C.; and •separating crude ethanol and wet solid transformation product from said mixture; further comprising that the incubation is performed as a continuous plug-flow process in a vertical, non-agitated, closed incubation tank with inlet means for said mixture and additives and outlet means for the solid transformation product and crude ethanol. The invention further relates to the products of this method as well as uses thereof.

Described herein is a beads-free bioprocessor as an automated and cost-effective T cell processing and manufacturing platform. T cells are a core component in CAR T cell therapies for cancer treatment, but are difficult to manufacture to scale in clinically relevant quantities. The 3D bioprocessor provides an alternative device that is scalable, beads-free, easy-to-use, and cost-effective for using CAR T cell therapy in cancer immunotherapy. Besides CAR T cell application, this platform technology has potential for many other applications such as cancer cell isolation.

A device to aid in the production and regeneration of tissues and organs, standing alone, or on or inside of the human body, with a method of fabrication of tissues and organs and use of the device.

Provided is a culture bag accommodating a culture fluid, the culture bag is capable of suppressing foaming in the culture fluid when oscillating the culture fluid to perform a culture, and performing a culture with high efficiency. The culture bag includes a culture space accommodating a culture fluid, the culture space being an endless space to allow the culture fluid to circulate therein, in which the culture bag has an inner surface that comes into contact with the culture fluid to be accommodated therein, the inner surface including, at least in part thereof, a first surface formed of a fine structure and a second surface formed of a structure different from that of the first structure.

The device for a cell suspension culture comprises a culture chamber comprising first and second axisymmetric-shaped walls having at least a partially curved radial section the concavity thereof facing the inside of the culture chamber, the walls delimiting an inner compartment and form an inner continuous surface; an inlet opening provided in the first wall and adapted to enable a fluid to enter; a supplying conduit connected to the inlet opening; and a valve member disposed inside the culture chamber adapted to tightly close or open the inlet opening. The device comprises also a device for moving the valve member, disposed outside the culture chamber.

A system for quickly determining antibiotic sensitivity of a microorganism comprising a triangular-shaped plate and cover for culturing, recovering, and re-suspending recovered microbial colonies and a second triangular-shaped plate comprising a non-liquid medium cut with concentric trenches over which the re-suspended microbial colonies are distributed by centrifugation and contacted with antimicrobial strips or disks.

A system and method for high density cell culture support utilizing two circulation circuits: a cell culture loop and a media conditioning loop.

A cell culture device comprising a fixed bed arranged in a housing, the fixed bed comprising a continuous pleated porous medium comprising a top end, a bottom end, a front side, a rear side, a right side, and a left side, and a plurality of pleats folded on the right side and the left side, and having vertical fluid flow channels along the longitudinal axis between adjacent pleats, is disclosed.

The invention pertains to surface topographies which can be used to modulate the morphology, proliferation, biochemical functioning, differentiation, attachment, migration, signaling, and/or cell death of a cell population by physical stimulation. Such topographies can be applied in vitro and in vivo to modulate cell behavior. Specific examples include implants provided with a topography of the invention which regulates the immune response, or an implant which increases osteogenesis. The invention furthermore pertains to objects which are used in vitro to modulate cell behavior.

A photobioreactor for cultivation and/or propagation of a photosynthetic organism and associated systems/methods are disclosed herewith. The photobioreactor includes (1) a substantially spherical vessel having a wall defining an interior vessel volume; (2) a water-submersible system for converting electrical energy into electromagnetic radiation; (3) a temperature management system for circulating heat dispersal fluid into and out of the water-submersible system; and (4) a photobioreactor control system comprising a processor and a controller.