Novel methods and apparatus are disclosed for cell culture and cell recovery. The methods and apparatus simplify the process of cell separation from media, minimize potential damage to gas permeable devices during fluid handling, and allow closed system automated cell culture and cell recovery from gas permeable devices.

The gas collection device includes a constant temperature chamber whose interior is maintained at a set temperature; a culture flask unit located inside the constant temperature chamber and culturing bacteria therein; a mass flow controller located outside the constant temperature chamber and connected to the culture flask unit through an injection flow path to control a gas injected into the culture flask unit through the injection flow path; and an impinger located outside the constant temperature chamber and connected to the culture flask unit through a discharge flow path to receive a gas discharged from the culture flask unit through the discharge flow path in real time.

In some aspects, the invention relates to automated cell culture incubators and their methods of use. In one aspect, the disclosure provides cell culture incubators having an airlock chamber, a storage chamber and/or an internal chamber. In some aspects, the disclosure provides methods for producing autologous mammalian cell cultures.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

A device for providing a sterile environment for the incubation of cell cultures is proposed, comprising a warming chamber with heating elements, sensors for temperature, humidity and CO2, and a gas supply providing a gas connection in the interior. The device also has a cultivation tank located in the warming chamber. This has a sealable passage for each sensor, and also a sealable gas inlet and outlet that couples to the gas connection inside the warming chamber. The sensors of the warming chamber are arranged to fit into respective passages of the cultivation vessel. The device also has a water reservoir arranged in the cultivation tank. The three elements of the warming chamber, the cultivation container and the water reservoir can be sterilized separately using the optimal method for each. Since the sensors are not part of the cultivation container, the latter can also be decontaminated by high temperatures. The sterilized warming chamber ensures that the cell cultures in the cultivation container cannot be contaminated from the outside, nor can they cause contamination in the laboratory.

The present disclosure provides a three-dimensional cell culture method and a cell culture apparatus using the same. The cell culture method includes lowering, when culturing cells on at least one surface of a porous support, a concentration of a cell-secreted substance in a lower region of the other surface of the porous support. The cell culture apparatus includes: an upper chamber including an opening, porous support having at least one surface on which cells are cultured, and a cell culture space in which a culture medium is housed; a lower chamber including a space in which at least one upper chamber is disposed; and a device lowering a concentration of a cell-secreted substance in a lower region of the other surface of the porous support.

A cell culture system or assembly, flask, plate and dish for growing, viewing and evaluating cells; and methods of use.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

A cell culture container includes: a container body; a cell culture insert having a tubular portion and an oxygen-permeable membrane; and a lid member. The container body is provided with an opening that communicates with an interior of the container body. The tubular portion includes an upper end and a lower end, and is inserted in the opening such that the lower end is located inside the container body. The lower end side of the tubular portion is closed by the membrane. The upper end side of the tubular portion is closed by the lid member. The lid member includes a lower surface that faces toward an interior of the tubular portion. The lid member is provided with an electrode insertion port and a culture medium outlet port that each communicate with the interior of the tubular portion at the lower surface.