A structured bag for cell culture is provided herein. The structured bag includes a top portion, a bottom portion having a shape, and a sidewall extending between the top portion and the bottom portion and at least partially defining an interior compartment for receiving fluid. The bottom portion is configured to maintain the shape to support the structured bag such that the structured bag is free standing when placed on a surface.

A cell enclosure device according to the present invention is a cell enclosure device for constructing a multicellular structure obtained by culturing cells, including a porous membrane in at least a portion of the cell enclosure device. In the present invention, a tissue-type chip includes the cell enclosure device in which one type of cells is enclosed. In the present invention, an organ-type chip includes the cell enclosure device in which at least two types of cells are enclosed. In the present invention, a kit for providing a multicellular structure includes an openable and closable sealed container including the tissue-type chip or the organ-type chip and a culture medium. In the present invention, an organ-type chip system includes at least two of the tissue-type chip or the organ-type chip, and the tissue-type chips or the organ-type chips are connected while maintaining a cell enclosure property. A method for culturing cells according to the present invention uses the cell enclosure device.

A cultivation bag assembly for the cultivation of microbes, fungi and other organisms includes an inner bag for containing a food substrate and an organism to be cultivated. At least one wall of the inner bag is constructed of a layer of water-vapor permeable material. An outer bag of the assembly has first and second walls formed from gas impermeable layers. At least one of the first and second walls of the outer bag has a gas filter patch to allow the passage of oxygen and carbon dioxide gases into and out of the interior of the inner bag to facilitate incubation of the organism within the interior of the inner bag. The inner bag and outer bag are coupled together at a first end of each of the inner bag and outer bag.

A bioproduct manufacturing system is disclosed. The system comprises a hollow-fiber primary membrane gas absorber comprising a shell side and a lumen, wherein the primary membrane gas absorber is configured to receive a carbonate salt-based solvent on the shell side and a gas mixture comprising oxygen, nitrogen, and carbon dioxide in the lumen, at least one runway algal cassette reactor-photobioreactor, and a growth medium circulating between the primary membrane gas absorber and the at least one runway algal cassette reactor-photobioreactor. The at least one runway algal cassette reactor-photobioreactor comprises at least one growth chamber coupled to and in fluid communication with a headspace channel so as to define and interior volume, a first condenser coupled to and in fluid communication with the headspace channel, and a harvest line in fluid communication with the at least one growth chamber and coupled to a filter.

Described herein are biofilm bioreactors for synthesis at the interface between two liquids, and methods of using such bioreactors for the biotransformation of feedstocks into chemical products. Also contemplated is the extraction of such products.

The invention concerns a cassette for cell culture comprising: an at least partially rigid housing internally defining an internal space inside which a cell culture bag defining an internal volume is arranged and attached; conduits each connected at one end to the internal volume of the bag and each having a second end situated outside the housing; and valves for enabling/preventing the flow of fluid through the conduits that are mounted on the housing.

The present invention provides a co-culture system and method for assessing cellular cholesterol (Choi) efflux and uptake in vitro. The co-culture system mimics in vivo Choi efflux and uptake in the context of mammalian physiology. The methods and systems provided can be used in some embodiments to evaluate the effect of a pharmacological agent on cellular Choi efflux and uptake or for diagnostic purposes.

A microfabricated device defining a high density array of microwells is described for cultivating cells from a sample. The device may be incubated to grow a plurality of cells, which may be split into an analysis portion and a reserve portion. Methods are provided for screening a biological entity of interest using the microfabricated device, for example, for screening phosphate solubilizing bacteria or other bacteria producing acids.

A cell sheet transfer device and a cell sheet processing equipment. The cell sheet transfer device includes a first tubular body; a second tubular body at one end of the first tubular body and connected thereto; and a cell shovel at the end of the first tubular body and on one side of the second tubular body. A free end of the cell shovel is beyond a free end of the second shovel body.

A biological sheet culture device includes a scaffold, a number of culture dishes for culturing biological sheets positioned on the scaffold and at least one fluid channel configured to traverse each culture dish.