A culture device and a culture method by which a substance to be cultured can be three-dimensionally cultured, and the substance thus cultured can be removed as an integrated product with multiple tubes; and a cultured organ produced by the culture method. The culture device comprises a sealed container (2) which contains the substance to be cultured (A) and is disassembled after the culture is completed; multiple ducts (3, 4, 5) arranged in the sealed container (2) and having a plurality of micropores formed on the outer peripheral surface; a culture medium-feeding device (6, 7) for feeding/circulating the culture medium (B) to at least one duct (3 or 5); an excretory device (8) connected to at least one duct (4) for excreting the waste product (C)permeating into the duct (4) through the micropores of the duct (4) from the substance to be cultured to the outside of the sealed container.

This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.

A cell culture vessel (100) includes a wall including an inner surface defining a cell culture chamber of the vessel. A substrate (215) of non-porous material is positioned in the cell culture chamber between a first region of the cell culture chamber and a second region of the cell culture chamber. The substrate includes a plurality of microcavities (220), each microcavity of the plurality of microcavities includes a concave surface defining a well and an opening, the concave surface of each microcavity includes at least one aperture including a dimension less than or equal to about 15 microns defining a path from the well to the second region. A cell culture vessel including a substrate including a plurality of microcavities and a layer of porous material is also provided. Methods of culturing cells in the cell culture vessel are also provided.

A dynamic incubator system and method for mammalian cells. The dynamic incubator system includes an incubator and a programming device communicatively coupled to the incubator. The programming device includes a memory, one or more processors, a display, and an input mechanism. The programming device is adapted to enable at least one preset temperature and gas concentration sequence to be one or more of designed for the interior of the incubator, saved to the memory of the programming device and/or selected for implementation within the interior of the incubator. The at least one temperature and gas concentration sequence includes programmed changes to the temperature and a CO.sub.2 gas concentration. A purging mechanism is coupled to the incubator and releases a portion of a gas concentration to enable rapid changes in the temperature and gas concentration in the incubator.

A continuous perfusion bioreactor for microbial synthesis of metallic nanoparticles provides synthesis of nanoparticles without washout of the microbial cells. The perfusion bioreactor includes an inlet fluidically coupled to a holding compartment with a first flow barrier, which disperses the medium into a reaction compartment suitable for culture of microbial cells which synthesize nanoparticles. A second flow barrier in the reaction compartment prevents washout of the microbial cells but enables collection of the nanoparticles suspended in depleted nutrient medium.

A cultivation bag assembly for the cultivation of microbes, fungi and other organisms includes an inner bag having first and second walls that are joined together along side edges to define an inner bag interior for containing a food substrate and an organism to be cultivated. At least one of the first and second walls is constructed of a layer of water-vapor permeable material to allow the passage of water vapor therethrough. An outer bag having first and second walls is joined along side edges to define an outer bag interior. The first and second walls of the outer bag are formed from gas impermeable layers. The inner bag and outer bag are coupled together at a first end of each of the inner bag and outer bag. The inner bag is contained within the interior of the outer bag in a cultivation configuration. A window is provided on the inner bag to allow one to view the interior of the inner bag through the window.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

A digital low dropout (LDO) voltage regulator enabling on-chip fine-grain power management in multi-core microprocessor and system-on-a-chip platforms to increase system level energy efficiency. Its design synthesizability with automatic placement and routing enables per-core dynamic voltage and frequency scaling with quick design turnaround. To enable per-core voltage regulation, the digital LDO is designed in a 65 nm complementary metal-oxide-semiconductor process. It exhibits core-level high load current driving capability of up to 125 mA and a large voltage regulation range of 0.15 V to 1.15 V.

3D cell cultures and devices for 3D cell culture, and methods of use thereof are provided. In some embodiments, the 3D cell culture comprise pancreatic β cells and can be generated in multi-well plates, allowing for high throughput assays on the cell culture.

A cell culture apparatus includes a culturing unit having a surface provided with a plurality of concave wells for culturing cells therein, and a flow unit including a gas flow path which allows gas to flow therethrough. The culturing unit includes a gas permeator that is arranged to separate the plurality of wells from the flow unit, the gas permeator having oxygen permeability and liquid impermeability.