In vitro equine organ model systems, and methods of making and using such systems, are provided and can include an organoid prepared using equine tissue associated with the organ of interest; or equine primary cells, wherein the equine primary cells are derived from equine tissue associated with an organ of interest, or derived from an organoid prepared using equine tissue associated with the organ of interest.

A method and apparatus for biogas extraction. The method includes obtaining a set of images that includes a field of view encompassing at least a portion of the flexible roof and a background; generating a sample region having a first end overlapping only the flexible roof and a second end overlapping only the background; determining an average pixel color of an end slice located at the first end or the second end of the sample region; determining, based on the average pixel color of the end slice of the sample region, a fill percentage of the sample region attributed to an object overlapped by the end slice; estimating an amount of inflation of the flexible roof based on the fill percentage and a correlation between fill percentages of the sample region and predetermined amounts of inflation included in calibration data; and controlling extraction of the biogas based on the estimation.

A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.

A method for carrying out a reaction process, in particular for setting the mixing and/or aeration of a reaction liquid while the reaction process is being carried out, which includes filling at least one reaction vessel with at least one reaction liquid, wherein an internal volume of the reaction vessel is not completely filled by the at least one reaction liquid at all times during the reaction process, and changing the internal volume of the reaction vessel in the course of the reaction process, in particular in a targeted manner, which causes a movement of the at least one reaction liquid.

The present invention discloses a piezoelectric ultrasonic microinjection device based on a flexible hinge mechanism. The device includes: a cover, a flexible hinge mechanism, a base, a screw cap and an end cap fixedly assembled together, the base being provided with a pump interface; and a micropipette fixedly mounted in the base, the screw cap and the end cap and extending outward, the micropipette being in communication with the pump interface; wherein the flexible hinge mechanism comprises a housing, a piezoelectric ceramic package module encapsulated in the housing, a central shaft fixedly mounted with the piezoelectric ceramic package module and the base, and a vibration output shaft extending from the piezoelectric ceramic package module into the central shaft, a plurality of flexible hinge beams being disposed between the central shaft and the housing.

A consumable container for filling with a fluid in a bioprocessing process. The container comprises one or more sealable, removable portions, such that one or more samples of the fluid may be taken by sealing and removing one or more of said portions.

A bioreactor for cultivating microorganisms and cells of animal or plant origin includes a filter pocket which is arranged on the inner surface of a flexible wall of the bioreactor and which is delimited on the outside by the wall of the bioreactor and on the inside by a filter pocket wall. The inside filter pocket wall is formed at least partially by a filter medium. A spacer is arranged in the filter pocket between the wall of the bioreactor and the filter medium. The spacer has at least one through opening. A connecting portion of the filter medium protrudes through the through opening and is directly connected to the wall of the bioreactor. A method of manufacturing a filter pocket in a bioreactor for cultivating microorganisms and cells of animal or plant origin is also provided herein.

An impeller assembly for a bioprocessing system includes a hub and at least one blade pivotally to the hub, the at least one blade including a first leg portion and a second leg portion extending at an angle from the first leg portion. The at least one blade is rotatable between a first position where the first leg portion extends generally outwardly from the hub and a second position where the second leg portion extends generally outwardly from the hub.

A flow distribution device for bioprocess systems, comprising: ⋅—a flow distribution manifold (12; 112; 212; 312) comprising: ⋅o at least four fluid connection tubes (14), wherein each fluid connection tube (14) comprises a first end (18) for fluid connection and an opposite second end (20) ⋅o a central common compartment (30; 130; 230; 330) to which the second ends (20) of each of the fluid connection tubes (14) are connected, whereby the first ends (18) of each of the fluid connection tubes (14) can be in fluid communication with the central common compartment (30; 130; 230; 330) ⋅—at least three pinching members (41) which are provided in connection with one fluid connection tube (14) of the flow distribution manifold (12; 112; 212; 312) each, wherein each of said pinching members (41) can be controlled into at least a first and a second position, wherein in the first position for each of the pinching members (41) the pinching member pinches one of the fluid connection tubes (14) such that fluid flow is prevented between the first end (18) and the second end (20) of this fluid connection tube (14)

A sparger assembly (700) for a bioprocessing system includes a base plate (710) and at least one aeration manifold (712, 714) removably connected to tire base plate. Each aeration manifold includes at least one inlet for receiving a gas and a plurality of gas outlet openings for delivering tire gas to a fluid within the bioprocessing system. An impeller assembly (740) fora bioprocessing system includes a hub and at least one blade (742) operatively connected to the hub. The at least one blade includes a first portion connected to the hub and extending generally vertically, and a second portion extending at an upward angle from tire first portion.