Tissue culture platforms that may be configured for tissue culture or biological cell culture, and methods for use thereof, are described. In general, the tissue culture platforms include multiple well chambers that are fluidically coupled by one or more channels Flow between the different well chambers is controlled via one or more selector valves, enabling a single tissue culture platform that can provide multiple integrated culture subsystems, multiple non-interacting culture subsystems, or combinations thereof.

A microfluidic device comprising a microfluidic network is described. The device comprises a base, a microfluidic channel and a cover, and the base comprises a diaphragm forming at least part of an inner surface of the microfluidic channel. The device finds use in methods for assessing mechanical strain induced in or by cells, such methods also being described.

Provided is a biological fluidic system that provides a high degree of sterilization by performing the biological processing in a closed system in which the fluid is contained in chambers that are isolated from the environment in a manner that does not permit ingress of contaminants.

A container system includes a flexible bag, a probe port, and a probe. The probe port includes an elongated tubular member having an interior surface bounding a first passageway extending between a first end and an opposing second end, the tubular member being made of a polymeric or elastomeric material and being flexible. The probe port also includes a flange coupled to the tubular member and radially outwardly projecting therefrom, the flange being secured to the flexible bag so that the first end of the tubular member projects into a chamber of the flexible bag while the second end of the first passageway of the tubular member is accessible from outside of the flexible bag. The probe can be received within the first passageway of the probe port.

A cell culture apparatus includes a culturing unit having a surface provided with a plurality of concave wells for culturing cells therein, and a flow unit including a gas flow path which allows gas to flow therethrough. The culturing unit includes a gas permeator that is arranged to separate the plurality of wells from the flow unit, the gas permeator having oxygen permeability and liquid impermeability.

The present invention provides mesh rolled scaffold devices and bioreactor systems that can provide a large surface-to-volume ratio for expanded cell culture. The mesh rolled scaffolds minimize shear stress on cultured cells and support sufficient and uniform mass transfer rates of gases and nutrients. The mesh rolled scaffolds can be connected to a media source via holders in bioreactor systems to support large-scale expansion and maintenance of cell cultures. The present invention also provides the bioreactor systems that can include dialyzers and heat exchangers to modify media and other fluids passing through the systems. The bioreactor systems include media and other fluid reservoirs that can support high stirring rates between about 100 and 10000 rpm, and the overall systems can be pressurized between about 1 and 10 atm to increase gas exchange rates.

The invention relates to a bioprocessing system having a main line (2) for conduction of a main flow (3) of a liquid, in particular biological, medium (45), having a main flow pump (5) for generation of the main flow (3) and having a system part (46) connected to the main line (2). What is proposed is that the bioprocessing system (1) comprises a powder transfer system (8) for metered delivery, in particular dust-free metered delivery, of an additive (47) and a feed line (9) which is connected to the powder transfer system (8) and which opens into the main line (2) at a feed point (10) and which comprises a return flow preventer (11) for prevention of a return flow of liquid medium (45), that the powder transfer system (8) feeds the additive (47) into the feed line (9) in powder form and drives it toward the feed point (10) by means of a pressurized transport gas (12) and that at least some of the fed additive (47) is input into the liquid medium (45) within the main line (2) downstream of the feed point (10) before the liquid medium (45) enriched with the additive (47) passes through or reaches the system part (46) connected to the main line (2).

Provided is a culture bag accommodating a culture fluid, the culture bag is capable of suppressing foaming in the culture fluid when oscillating the culture fluid to perform a culture, and performing a culture with high efficiency. The culture bag includes a culture space accommodating a culture fluid, the culture space being an endless space to allow the culture fluid to circulate therein, in which the culture bag has an inner surface that comes into contact with the culture fluid to be accommodated therein, the inner surface including, at least in part thereof, a first surface formed of a fine structure and a second surface formed of a structure different from that of the first structure.

A filter assembly for separating microcarriers from a fluid medium includes a collapsible container bounding a sterile compartment adapted to hold a fluid. An inlet port is attached to the container through which fluid flows into the compartment. An outlet port is attached to the container through which fluid flows out of the compartment. A filter is disposed within the compartment, the filter dividing the compartment into an inlet chamber that is fluidly coupled with the inlet port and an outlet chamber that is fluidly coupled with the outlet port, the filter allowing a medium to pass therethrough but preventing microcarriers disposed in the medium from passing therethrough.

The present invention contemplates compositions, devices and methods of simulating biological fluids in a fluidic device, including but not limited to a microfluidic chip. In one embodiment, fluid comprising a colloid under flow in a microfluidic chip has a fluid density or viscosity similar to a bodily fluid, e.g. blood, lymph, lung fluid, or the like. In one embodiment, a fluid is provided as a Theologically biomimetic blood surrogate or substitute for simulating physiological shear stress and cell dynamics in fluidic device, including but not limited to immune cells.