The present disclosure describes a manufacturing apparatus to use algae as a renewable green factory for producing biodegradable bioplastic. One or more embodiments include separation of a cultivated microalgae biomass from water before use in the wet or dried state. The lipids and proteins are extracted from the biomass which leaves starch and algae precursors in the remaining material from the microalgae cells. The starch includes amylose, amylopectin, monosaccharides kinases and cyclobutadiene and is hydrolyzed into a syrup containing oligosaccharides and polysaccharides. In some cases, the syrup is used as an ingredient in a medium containing nutrient for bacterial fermentation of plastics.

Improved methods for anaerobic digestion of organic matter to produce biogas. Among the improvements given are including ferric iron in a hydrolysis reactor to increase the rate and efficiency of anaerobic hydrolysis to provide substrates for methanogenesis. A solids separation step is added after hydrolysis and before methanogenesis to improve the efficiency of the methanogenesis step. Other improvements involve using separate tanks for the hydrolysis and methanogenesis stages and using two (or more) methanogenesis tanks in sequence, and switching the order of the two (or more) methanogenesis tanks periodically.

A system and method for monitoring or controlling an aerobic landfill bioreactor having a plurality of zones each including at least one well head. The system comprises: a gas extraction blower, a plurality of temperature sensors disposed in the well heads, an ex-situ relative humidity detector, an ex-situ gas constituent detector, an air injection system, a liquid injection system, and a controller. Landfill gas is extracted by the blower and it's temperature, relative humidity, and gas constituents are measured. The system further comprises a system of headers, isolation valves, and flow control valves which serve to isolate zones for measurement and/or air and liquid injection as well as control the flow of either the landfill gas extraction or liquid and air injection. The method involves measuring: temperature, relative humidity, and gas constituents of the extracted landfill gas. Advantageously, the ex-site placement of the sensors and detectors ensure that measurements are representative of the aerobic bioreactor operating parameters. The air and liquid injection systems are adjusted, based on the measured temperature, relative humidity, and gas constituents to optimize the operation of the aerobic landfill bioreactor.

A culture container transportation set suitable for cultured state-maintaining transportation is provided. A culture container transportation set A1 includes: a culture container 13 including a vessel 13 made up of a bottom wall 11 and a tubular side wall 12 rising from the bottom wall 11; a flexible cover 2 covering an upper edge portion 121 of the side wall 12; a hard pressing member 3 provided on the cover 2; a cushioning material 4 of shape restorability; and a housing container 6 that houses the culture container 1, the cover 2, the pressing member 3 and the cushioning material 4 in an assembled state in which these components are stacked while pressing these components from above and below. The cushioning material 4 is provided between the housing container 6 and the culture container 1 or between the pressing member 3 and the housing container 6.

A renewable energy microgeneration apparatus is disclosed that includes a mixing tank that mixes waste with a liquid, a buffer tank that receives and pre-warms the mixed waste, a pasteurization tank that pasteurizes on the pre-warmed mixed waste, a digestion tank that performs anaerobic digestion on the pasteurized waste, a de-watering device that separates liquid digestate and removes salt from the liquid, sensors that measure salinity and biogas quality, and a controller. The controller causes the transfer of digestate from the digestion tank to the pasteurization tank to the dewatering device, causes the de-watering device to separate the liquid and remove the salt from the liquid, monitors the salinity of the liquid and the quality of biogas using the sensors, and causes the mixing of the liquid with the waste and adjusts the feed rate of the waste to reduce the salinity of the waste and increase methane production.

A movable cell incubator contains: a body, a first lid, a second lid and an electric control unit. The body includes a first internal space, a refrigeration room, and an airtight culture room. The first lid airtightly covers the culture room, the second lid airtightly covers the refrigeration room, and the control unit includes a microprocessor, a power module, a digital/analog conversion module defined between a microprocessor and the power module, a heating module controlling temperature of the culture room, a cooling module supplying cold source to the refrigeration room, a peristaltic pump module, a flow sensing module, a CO2 detective supply module supplying CO2 to the culture room, and a setting display module exposing and fixed on the first lid, with the peristaltic pump module aseptically connected between cell culture media and cell culture bag by multiple conveying tubes.

An integrated system of cell culture and operation and a method for controlling the same. The integrated system of cell culture and operation includes a housing, a front side of the housing being provided with operating gloves. The housing has a double-layer structure consisting of an inner layer and an outer layer. An upper wall and a lower wall of the inner layer are both provided with through holes. The outer layer has a gas inlet, a gas outlet and a humidifying port. The integrated system of cell culture and operation further includes: a heating module arranged in an interlayer, and a transport module configured to transport gas and circulate the gas in the inner layer and the interlayer, resulting in low gas consumption.

Methods of keeping microorganisms working at maximum efficiency in an anaerobic digester to produce a renewable biogas, within a temperature range of 100 degrees F., preferably at a plus or minus of 0.5 degrees F., without adding heat to the mixture. Most preferably, the method includes premixing a batch of biomass including the microorganisms to form a uniform mixture, which also can include preheating the mixed biomass to approximately 100 degrees F. The digester operation identifies the rate that the mixture in the digester can be changed to a different mixture. Furthermore, the method can include separately collecting the CH.sub.4 biogas and the CO.sub.2 biogas in different chambers to meet the desired concentration of each these gases and can include removing from the biogas any sulfur containing gas and liquid water, and additionally separating lighter and/or heavier than water non-organic materials prior to them entering the digester.

Culture systems and methods of using same. The systems include a housing defining an inner space. The inner space includes a headspace and at least a portion of a reservoir. A surface for immobilizing cells is moveable between the headspace and the reservoir. The systems can be used for coculturing methanotrophs and phototrophs for processing biogas and wastewater, particularly from anaerobic digesters.

An industrialized protein production system using carbon-containing industrial gas includes a bacteria preparation system, a raw gas purification system, a water purification system, a bacteria separation system and a protein preparation system, wherein the bacteria preparation system is respectively communicated with the raw gas purification system, the water purification system and the bacteria separation system, and the protein preparation system is communicated with the bacteria separation system. By purifying the raw gas and the raw water and removing impurities from the raw gas and competing bacteria in the raw water, excellent raw materials and environment are provided for bacterial reproduction, which enable the raw gas to have high-efficiency fermentation, thereby increasing the yield of proteins.