Disclosed is a bioprocess system (1) comprising a flow path (3; 3a, 3) comprising: a plurality of inlets (7); at least one bio-processing device inlet connection (9a) and at least one bioprocessing device outlet connection (9b); a plurality of outlets (11); and flow path parts (5a′, 5b′, 5c′, 5d′, 5e′) of at least some active components (5a, 5b, 5c, 5d, 5e) comprised in the bioprocess system; and active parts (5a″, 5b″, 5c″, 5d″, 5e″) of said active components (5a, 5b, 5c, 5d, 5e). The flow path (3; 3a, 3b) of the bioprocess system is positioned centrally within the bioprocess system (1) and in a vertical orientation, whereby the active parts (5a″, 5b″, 5c″, 5d″, 5e″) are positioned around the flow path (3; 3a, 3b) being accessible from outside the bioprocess system (1) and each in connection with a corresponding flow path part (5a′, 5b′, 5c′, 5d′, 5e′) of an active component (5a, 5b, 5c, 5d, 5e).

The invention relates to a sterile connector for the sterile transfer of a liquid medium, in particular a biological medium, from a liquid container (2) into a fluid chamber (3, 3), wherein the sterile connector (1) has a first coupling device (5) and a second coupling device (6). It is proposed that the first coupling device (5) has a fluid inlet (7) and a fluid outlet (8) and also a first cannula (9), the latter having an end which is directed away from the fluid inlet (7) and forms the fluid outlet (8), that the second coupling device (6) has a fluid passage (10) which, in an initial state of the sterile connector (1), is covered axially by a first septum (11), that the fluid outlet (8), in the initial state of the sterile connector (1), is arranged in a hermetically sealed region of the first coupling device (5) and the surface (11a) of the first septum (11) facing axially away from the fluid passage (10) is arranged in a hermetically sealed region of the second coupling device (6), and that, in a fluidic connection process starting from the initial state of the sterile connector (1), a fluidic connection between the fluid inlet (7) and the fluid passage (10) can be produced by the fact that the end of the first cannula (9) forming the fluid outlet (8) pierces the first septum (11) of the second coupling device (6).

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

A cell collecting device include a pipette tip that sucks substances in a cell culture container, a first valve connected to the pipette tip through a first flow path, a first pump connected to the first valve through a second flow path, and a second pump connected to the first valve through a third flow path. In a remove mode, the first valve is switched to connect the first flow path and the second flow path to each other and disconnect the third flow path, and the first pump is driven to discharge waste in the cell culture container, that has been sucked from the pipette tip, from a drain of the first pump through the first flow path and the second flow path, and in a picking mode, the first valve is switched to connect the first flow path and the third flow path to each other and disconnect the second flow path, and the second pump is driven to suck cells in the cell culture container using the pipette tip.

Embodiments of the invention relate to a Disposable Bioprocess System including a Single-Use-Bioreactor, a Single-Use-Pump and a single-use micro-organism retention filter. Combined most suitable for cultivation of suspended micro-organisms in a liquid media at high micro-organism concentration in a perfusion mode continuous process for expression of biological material. The inlet port of the liquid Single-Use-Pump connects via a valve to the broth reservoir of the Single-Use-Bioreactor through a liquid conveying port. The outlet port of the liquid pumping Single-Use-Pump connects via a valve to a micro-organism retention filter. And a method for operating said sterile Disposable Bioprocess System in perfusion mode for continuously processing.

The present set of embodiments relate to a system, method, and apparatus for hydrating and mixing a liquid medium from a dry medium. Such systems, methods, and apparatuses can be used in the biotech industry because they can be used to provide prepackaged dry media in a format that can be quickly rehydrated free of issues with operator error found in more conventional media rehydration systems using mixing tanks and reactors.

Digital microfluidic (DMF) methods and apparatuses (including devices, systems, cartridges, DMF readers, etc.), and in particular DMF apparatuses and methods adapted for large volume. For example, described herein are methods and apparatuses for DMF using an air gap having a width of the gap that may be between 0.3 mm and 3 mm. Also described herein are DMF readers for use with a DMF cartridges, including those adapted for use with large air gap/large volume, although smaller volumes may be used as well.

A variety of fill options is provided for a cell processing system. Certain options relate to a syringe assembly that receives a product directly from a separator. Other options relate to a filling system associated with the cell processing system, the filling system comprising one or more filling stations, each with at least one container, that receive product from a product container associated with the cell processing system.

A bioreactor system, includes a base platform, a lid received atop the base platform and defining an interior space for receiving a bioprocessing bag, and a tubing management system supporting a tubing array a distance above the base platform and providing a means for quickly connecting and/or disconnecting a fluid supply line.

A biological component treatment system is equipped with a biological component kit having a path through which a culture medium is capable of flowing, and a biological component treatment device that controls a flowing state of the culture medium. In a cell culturing method, there are performed a measurement step of performing a measurement of information in relation to lactic acid, a cell number calculation step of calculating a number of cells during culturing of cells on the basis of the obtained information in relation to lactic acid and calibration information, and an operation control step of controlling supply of the culture medium to the cells on the basis of the number of cells.