A method of dispensing fluid includes dispensing a first quantity of fluid so that fluid flows through a manifold port assembly and into a first container, the manifold port assembly includes a housing having an inlet port and a plurality of outlet ports formed thereon; and a carousel bounding a plurality of fluid paths that each extend between a first end and an opposing second end, the carousel being rotatably disposed within the housing so that when the first end of each one of the plurality of fluid paths is aligned with the inlet port on the housing, the second end of the corresponding one of the plurality of fluid paths is aligned with one of the plurality of outlet ports. A motor is activated to rotate the carousel of the manifold port assembly to a second position. A second quantity of fluid is dispensed so that fluid flows through the manifold port assembly and into a second container.

A flow distribution device for bioprocess systems, comprising: a flow distribution manifold (12; 112) comprising: at least four fluid connection conduits (14), wherein each fluid connection conduit (14) comprises a first end (18) for fluid connection and an opposite second end (20), and wherein at least three of the fluid connection conduits comprise a membrane (19a) and a valve seat (19b), which membrane (19a) can be put in at least two different positions in relation to the valve seat (19b) for allowing or preventing fluid flow between the first end (18) and the second end (20) of the fluid connection conduit (14); and a central common compartment (30) to which the second ends (20) of each of the fluid connection conduits (14) are connected, whereby the first ends (18) of each of the fluid connection conduits (14) can be in fluid communication with the central common compartment (30) and wherein the fluid connection conduits (14) are entering the central common compartment (30) from at least three different directions; wherein said flow distribution device (10) further comprises at least three membrane actuation members (41) which are provided in connection with one membrane (19a) of the flow distribution manifold (12; 112) each, wherein each of said membrane actuation members (41) is configured for actuating the membrane (19a) to be in at least two different positions in relation to the valve seat (19b), wherein a first position of the membrane (19a) allows flow between the first end (18) and the second end (20) of the fluid connection conduit (14) and a second position of the membrane (19a) prevents flow between the first end (18) and the second end (20) of the fluid connection conduit (14).

The present invention relates to a computer implemented method for determining a weld integrity test result performed by a system configured to aseptically transferring cells to a container (BR), the method comprising welding (710) a vial to a connector to obtain a fluid-tight seal between the vial (V) and a connector (C), the connector being provided with a pair of conduits each having one end protruding through the connector and into the vial, sealing (720) an opposite end of one of the conduits, generating (730) a fluid pressure different to an ambient fluid pressure at an opposite end of the other conduit, measuring (740) a fluid pressure within at least one of the conduits, determining (750) a fluid pressure change based on the ambient pressure and the measured fluid pressure, determining (760) the weld integrity test result as pass or as fail based on the fluid pressure change.

A liquid substrate tank for a biogas plant includes an interior fillable with a liquid substrate and a bottom wall defining the interior on the bottom and being particularly at least regionally flat. A trough-shaped recess extending over a partial region of the bottom wall is formed in the bottom wall, to which and/or into which recess an extraction line of an extraction device is guided. The extraction device has an open-loop and/or closed-loop control device actuating the extraction device for extracting a substrate/sand mixture accumulating in the recess during operation from the recess and thus from the interior through the extraction line. The extraction device has an accommodation and/or sedimentation tank, or separator, connected to the extraction line relative to flow for accommodating or separating the substrate/sand mixture extracted through the extraction line into substrate and sand phases.

A cell culture system of the present invention includes: a culture-medium storage part that holds a culture medium for culturing cells; a temporary holding part that is coupled to the culture-medium storage part and that is provided with a holding space for temporarily holding the culture medium supplied from the culture-medium storage part and a discharge port for discharging the culture medium; a culture-medium supply part that is coupled to the discharge port of the temporary holding part and that supplies the culture medium discharged from the temporary holding part, to a cell culture vessel; and a discharge part that discharges the culture medium from the cell culture vessel, wherein the culture-medium supply part is provided with a culture-medium intermittent supply mechanism that intermittently supplies the culture medium discharged from the temporary holding part, to the cell culture vessel in a predetermined cycle.

The present invention relates to a mixing system for a bioreactor, comprising a plurality of supply units (10), each being able to hold media for use in a bioreactor a mixing unit (30) for creating a uniform mixing of media a first feeding mechanism (20), arranged to feed media from the supply units (10) into the mixing unit (30), a control unit (40) operatively connected to the first feeding mechanism (20) and the mixing unit (30), said control unit (40) being configured to control the first feeding mechanism (20) to feed predetermined amounts of media from the plurality of supply units (10) to the mixing unit (30), and further being configured to control the mixing unit (30) to create a uniform mixing of media. The invention also relates to a method for mixing media for use in a bioreactor.

A device including a mixing bag for mixing liquids, a cell-suspension bag fluidly connected to the mixing bag, a diluent bag fluidly connected to the mixing bag, and a fluidly connected to the mixing bag is capable of easily preparing a sheet-shaped cell culture without requiring any large-scale equipment. The sheet-shaped cell culture can be aseptically prepared by the device, while reducing the risk of contamination, by using the mixing bag which is fluidically connected to the other bags. The device includes a support having a foldable structure. At least one of the cell-suspension bag, the diluent bag and the filter is fixed to various portions of the support. The device can be folded into a compact size, by folding the various portions of the support, allowing for efficient transportation and storage.

An automated biological growth and dispensing apparatus utilizing a modular growing tank which is removable for replacement by another growing tank. Mechanisms for the delivery of air, water and/or nutrients are adapted to permit the growing tank to be readily coupled and uncoupled for easy and inexpensive replacement. Mechanisms for agitation may be integral and removable with the growing tank or may be adapted for a quick connection and disconnection with the growing tank. The growing tank may be disposable and each new growing tank may be provided as a sealed container including a starting amount of a biomass and/or nutrient.

Scalable biomaterial-based bioreactors are described. In one embodiment, the bioreactor may comprise perforated plates stacked such that the assembled bioreactor has the necessary manifolds and chambers to transport gas and liquids to a biomaterial contained within the bioreactor, and to remove the reaction products. In another embodiment, single use bioreactors are described. Methods of operating the bioreactors are also described.

A bioreactor for preferably three-dimensional cell culturing comprises a scaffold chamber, a first tube, a second tube and a first valve with a scaffold adapter, a tube adapter and a medium adapter. The first valve has a housing with a longitudinal female portion ending in an opening and a longitudinal actuator being arranged through the opening of the female portion of the housing such that the actuator is arranged partially inside the housing and partially outside the housing, wherein the actuator of the first valve is axially moveable relative to the housing of the first valve between a first position in which the first valve is in the operation position and a second position in which the first valve is in the medium change position. By providing the actuators in the first valve which is applied by axial movements, operation of the bioreactor can be comparably simple and safe.