Microfluidic devices, systems, and methods.

A system for isolating a target molecule from a bioprocess fluid includes a single-use disposable separation device having a plurality of perimeter-bonded layers defining one or more mesofluidic channels of the separation device, wherein each layer includes a biocompatible polymer material, wherein the separation device is configured to separate at least a portion of particles from the bioprocess fluid to generate a substantially clarified bioprocess fluid, and a chromatography system fluidically coupled at the outflow of the separation device in a configuration for further processing the clarified bioprocess fluid.

A cell culture system includes a culture medium container to supply a culture medium to a culture container, two culture containers for cell culture, and a transfer tube to connect these containers, whereby liquid delivery is performed by one pump. The containers are connected to the transfer tube; the one pump is attached to the transfer tube; the second culture container is connected to the transfer tube on both sides of the pump to connect the second culture container and the transfer tube, to inject cells into the first culture container; the culture medium is transferred from the culture medium container to the first culture container by the pump; after the cell culture in the first culture container, a cell suspension is transferred from the first culture container to the second culture container; and the culture medium is transferred from the culture medium container to the second culture container.

Some fluid coupling devices described herein are configured for use in fluid systems. For example, some embodiments described in this document are single-use, aseptic fluid coupling devices that can be coupled to create a sterile flow path therethrough. Some such aseptic couplings are genderless couplings such that two identical aseptic couplings can be coupled together and then latched to form a robust connection between the aseptic couplings.

There is provided a connector, for introducing or extracting a material to or from at least one receptacle, comprising a housing extending between a distal end and a proximal end, the housing comprising, at least at one end, a pierceable seal; a hollow needle mounted, at least partially, within the housing between the distal end and the proximal end of the housing, a first end of the hollow needle being connected or connectable to a first corresponding receptacle, and a second end of the hollow needle facing the pierceable seal at an end of the housing; and an actuating mechanism acting on the housing or the hollow needle to enable the hollow needle to pierce the pierceable seal thereby forming a communication through the pierceable seal, such that material is able to transfer through the connector.

A microfluidic device may include a microfluidic channel including an electrode placed at opposite ends of the microfluidic channel to create an electrical field within the channel and an ejection device to eject at least one cell porated within the electrical field. A cassette may include a substrate, a die coupled to the substrate, a microfluidic channel defined within the die, the microfluidic channel including a necked portion to receive a cell therein and at least two electrodes each placed at a first and a second end of the microfluidic channel to apply an electric field to the cell above a proration threshold and a cell ejection device to eject the cell from the die.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

An automatic in vitro cell culture platform includes a fluid storage device, a channel control panel, a cell culture chip and a fluid drive unit. The fluid storage device is for storing a cell culture medium and a waste liquid. The channel control panel is pipe-connected to the fluid storage device. The cell culture chip is disposed on the channel control panel and is for culturing a cell. The fluid drive unit is pipe-connected to the channel control panel and is for driving the cell culture medium and the waste liquid to flow between the fluid storage device and the cell culture chip through the channel control panel.

The present invention relates to bioreactor system and method thereof wherein support matrix (2) comprises at last one central shaft and plurality of peripheral shaft being radially surrounds central shaft. Arrays of discs (11) are mounted along the shaft by defining interspatial vicinities between two successive plates. Thus, discs mounted on peripheral shafts are rotated within the interspatial vicinity of discs of central shaft to ensures sufficient mixing and avoid stagnant fluidic zones which is created when discs are mounted closely apart from each other on shafts. Further, plurality of deflector vanes that are axially provided along the length of the central shaft to redirect substantially co-axial direction fluid flow into interior of culture vessel and more specifically towards the central axis. Thus, bioreactor system provides scalable and disposable bioreactor with efficient mixing and homogeneous conditions and thereby supports high density growth and maintenance of cells and other biological material.

A measuring device for a container having a process connection for determining the concentration of a constituent in an essentially liquid container content, comprising a concentration sensor, wherein the measuring device comprises a filter, an extraction line and a pump, wherein the filter is arranged within the container and the concentration sensor is arranged outside of the container, and the extraction line runs, starting from the filter, through the process connection to the concentration sensor, with the concentration sensor being connected to the pump, and the pump conveys the container content from the container through the filter across the extraction line to the concentration sensor, with the extraction line having at least one bend located between the filter and the process connection.