The present invention features filtration assemblies, cassettes, systems, and methods for filtering cells from a sample solution and then incubating the captured cells.

A cell culturing system (100) comprises a docking station (104, 105), a handling unit (102), a culturing module (101) and an actuation layer. The culturing module has a culturing well and a culturing membrane separating the culturing well in an apical culturing chamber and a basal culturing chamber. The handling unit has a seat for removably accommodating the culturing module and the actuation layer and a bottom with an actuation bore, wherein, when the culturing module is arranged in the seat, the actuation bore is associated to the culturing well and the bottom is separated from the culturing module by the actuation layer. The docking station has a coupling structure for removably holding the handling unit in a predefined position and an actuation feeding channel, wherein, when the handling unit is held by the coupling structure in the predefined position, a first end of the actuation feeding channel is connected to the actuation bore and a second end of the actuation feeding channel is connected to a connector.

Compositions of matter, methods, modules, and automated instruments may relate to synthesizing a library including an editing cassette including a different gRNA and donor DNA pair, amplifying the editing cassette in a partition separate from other editing cassettes in the library, adding nuclease to the partition, and adding lipofectamine to the editing cassette and nuclease to form a lipofectamine/nucleic acid/nuclease complex. A microcarrier coated in extracellular matrix or a cell adhesion molecule coating may be added to the lipofectamine/nucleic acid/nuclease complex. Cell growth material, the microcarrier, and mammalian cells may be transferred to a growth module in an automated closed cell editing instrument via a liquid handling system. The mammalian cells may be allowed to seed on the microcarrier. Conditions may be provided for the mammalian cells to take-up and be edited by a payload associated with the lipofectamine/nucleic acid/nuclease complex. The mammalian cells may be detached from the microcarrier.

Systems, devices, and methods for producing a tissue specimen and performing subsequent biomechanical testing thereof to quantify various biological/physiological properties and characteristics. The system may include a bioreactor device for producing the tissue specimen which includes a packaged/sealed seeding cup containing a scaffold in a sterile environment amenable to seeding with cells. After seeding, the cup may be stored in a standard laboratory culture tray for incubation. The produced tissue specimen may next be transferred to a testing instrument under control of a microcontroller which automatically performs a series of tests on the tissue and generates testing data communicated to an external electronic device which can operably interact with and control operation in part of the testing instrument via software. Tissue integrity in the instrument may be maintained by sterile fluid circulation and heating systems. The system advantageously provides efficient and reproducible tissue growth and analysis.

An incubator (1) and its use for cultivating microorganisms and/or for carrying out a chemical reaction are provided. The incubator (1) has a sample vessel holder (3) for insertion of a sample vessel (4), a heating device (5) for changing a temperature in the sample vessel holder (3) and/or in a sample vessel (4) inserted into the sample vessel holder (3), and a sample-inactivating device (6) for inactivation of a sample (7) in a sample vessel (4) that is inserted into the sample vessel holder (3).

Disclosed are an automated nucleic acid extraction method and device. The device includes a base body, a cassette, a driving unit, a moving frame and a syringe. The base body comprises a sample accommodating area, a column accommodating area, a cassette accommodating area and a collection tube being arranged in a linear direction. The cassette is arranged in the cassette accommodating area and includes two parallel walls and at least two vertical walls. The parallel walls and the vertical walls jointly form a lysis buffer well, at least one wash buffer well and an elution buffer well. Each vertical wall includes a load-bearing abutment. The driving unit and the moving frame are arranged on the base body. The syringe is arranged on the moving frame and is driven by the driving unit to reciprocate along with the moving frame in the linear direction.

A cell processing system, fluidics cartridge, and methods for using actuated surface-attached posts for processing cells are disclosed. Particularly, the cell processing system includes a fluidics cartridge and a control instrument. The fluidics cartridge includes a cell processing chamber that has a micropost array therein, a sample reservoir and a wash reservoir that supply the cell processing chamber, and a waste reservoir and an eluent reservoir at the output of the cell processing chamber. A micropost actuation mechanism and a cell counting mechanism are provided in close proximity to the cell processing chamber. A method is provided of using the cell processing system to collect, wash, and recover cells. Another method is provided of using the cell processing system to collect, wash, count, and recover cells at a predetermined cell density.

The invention is comprised among apparatus and/or devices for manipulating and handling fluids. The invention relates to the integration of these apparatus and/or devices in a system for manipulating and/or handling any type of fluids. Specifically, the invention relates to a disposable cartridge and a platform cooperating with one another in a flexible system for manipulating fluids, as well as a flexible system for manipulating fluids. More particularly, the present invention is intended for culture cells.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

A system for processing biological material includes a first module and at least a second module, in which each of said first and second modules has at least the following identical characteristics: a size and a shape of a housing; a storage device being rotatably located within said housing; and a plurality of plate slots located in the storage device for receiving plates or containers carrying pipette tips, cell cultures or liquids. The first and second modules are selected from a process module, a storage module, an incubator, a freezer, a plastic-ware store, a refrigerator or a centrifuge.