A kit for a bioreactor includes a bioreactor bag, a connector, an impeller assembly, and a clamp. The connector defines an aperture. The connector also includes a welding surface extending between a first end and a second end, with the second end including an outwardly extending flange. The welding surface can be heat-sealable to the bioreactor bag. The impeller assembly includes a shaft and a blade configured to pass through the aperture. The impeller assembly includes an impeller flange configured to contact the flange of the connector when the impeller shaft is received in the aperture. The impeller assembly also includes a bearing supporting the impeller shaft. The clamp is configured to secure the connector flange to the impeller assembly. Preparing a bioreactor includes welding the connector to the neck of the bioreactor bag and then inserting the shaft and blade through the aperture into the bioreactor bag.

The present disclosure relates generally to a tubing apparatus for separating and concentrating stem and stromal cells, also known as regenerative cells, from adipose tissue, more specifically to a defined process of extracting, separating and concentrating clinically useful regenerative cells from adipose tissue using a combination of mechanical disruption and filtration-centrifugation to obtain a highly enriched heterogeneous population of stem stromal cells. The centrifuge tube comprising a threaded top cap with male luer access port to be adapted to female luer of a syringe, a tapered main tubular barrel, a thin disk filter, and a bottom conical cap with luer access port for withdrawal of stem stromal cells via a syringe.

The invention relates to a harvesting device (100, 100a, 100b, 100c, 100d) for harvesting a content of a bioreactor bag (102) and to a method for harvesting a content of a bioreactor bag (102). The harvesting device (100, 100a, 100b, 100c, 100d) comprises: a first fixing device (104) for fixing one of the fixing portions (112, 114) to the harvesting device (100, 100a, 100b, 100c, 100d), a second fixing device (106) for fixing the other of the fixing portions (112, 114) to the harvesting device (100, 100a, 100b, 100c, 100d), a base (108), and an adjusting device (110) coupling the first fixing device (104) and/or the second fixing device (106) to the base (108). The adjusting device (110) is configured to selectively allow changing an arrangement of the first fixing portion (112) and/or the second fixing portion (114) relative to the base (108) or fixing the arrangement of the first fixing portion (112) and/or the second fixing portion (114) relative to the base (108).

A holding tool is configured to efficiently and reliably enable a close contact between a container and a lid with a simple mechanism and a simple operation. The holding tool is configured to be used together with a container and a lid for the container to sandwich the container and the lid. The holding tool includes a base on which the container is placed, and a cover configured to cover the lid. The base and the cover each have an opening at a central portion and are configured to engage with each other to press and sandwich the container and the lid upward and downward.

The present invention relates to systems and methods involving interconnected plate components, including bases, lids and covers, interconnected in a manner such that a continuous strip of each component is prepared. The continuous strips may be stored as rollstock in a reel style. The physical properties of each continuous strip allow the base, lid and/or cover to include means for positive control. In one embodiment, the continuous strip of bases is advanced and processed through an automated system with positive control, and remains in the form of a continuous strip until agar in the bases is cured, at which time the bases are singulated. When a lid is applied to a base using methods described herein, an airtight seal is formed improving the quality of culture media used in testing.

A device for cell culture comprising: a main body (11); said main body (11) comprises a plurality of circular portions (21-23); said device comprises a plurality of caps (51-53); each of said plurality of caps (51-53) comprises a base structure (58) having a circular hole (57), housing an upper slide (54) and an elastomeric layer (56) secured to said slide (54); said elastomeric layer (56) has a rectangular hole (59); said plurality of caps (51-53) being adapted to cooperate with said plurality of circular portions (21-23); each of said plurality of circular portions (21-23) each comprise an inlet hole (64-66) and an outlet hole (67-69) aligned with the long side of said rectangular hole (59), to perfuse the culture chamber (12-14) located in said rectangular hole (59) of said elastomeric layer (56).

The invention discloses a first connection unit having a plurality of sensor surfaces and which is adapted to be aseptically connected to a second connection unit mounted e.g. on a flexible bioreactor bag.

A pack includes at least one stack with Petri dishes which extends in a longitudinal direction, a connecting device which holds the at least one stack together, and an outer packaging which surrounds the at least one stack and which can be opened to remove the at least one stack.

Bioreactor bags formed from sheet materials are disclosed. The bioreactor bags include an opening which is openable and recloseable in a fluid tight manner for providing an opening for insertion of solid materials into the bag.

One aspect of the invention provides a multichamber bioreactor. The multichamber bioreactor includes multiple planar layers stacked on each other defining at least one chamber and a clamping mechanism. The clamping mechanism includes a housing and retaining means received in the housing and configured to generate a controlled and uniform pressure to secure the stacked multiple planar layers in the housing. Each chamber is implemented from a separate fluidic layer, with each fluidic layer having ports and valves independent of the other layers. The micro fluidic ports can be actuated through a micro fluidic interconnect system utilizing rotary cylinder valves.