This invention concerns an integrated microfluidic system that utilizes microfluidic chip technology to receive a patient sample including cells, expand the cells, reprogram the expanded cells and then store the reprogrammed cells in a microfluidic chip. These microfluidic chips with stored reprogrammed cells may then be used in scenarios of genetic differentiation into specific cell types. Overall this system and workflow is suitable as a hospital based device that will allow the generation of iPSCs from every patient for downstream diagnostic or therapeutic use.

A compound algae culture apparatus is provided, which includes a photobioreactor module, a growth regulating module, a circulation transfer module, and a circulation pipeline module. The photobioreactor module includes at least one photobioreactor unit, and the growth regulating module includes at least one growth tank unit. The photobioreactor unit includes a light-transmitting coiled pipe. The growth tank unit has a tank body, and a plurality of partitions are disposed in the tank body to divide an inside of the tank body for formation of a curved flow channel A culture fluid for culturing algae passes through the photobioreactor unit and enters the growth tank unit. A volume of the growth tank unit is larger than a volume of the photobioreactor unit, and a residence time of the culture fluid in the growth tank unit is greater than a residence time of the culture fluid in the photobioreactor unit.

The present invention provides a buffer preparation and transfer system for a process of manufacturing an antibody pharmaceutical which can reduce the input of a lot of labor due to the preparation of each buffer, including the weighing of a buffer chemical, the input of a powder, stirring and transfer, and significantly shorten process time by positioning and designing components in a manner that automates the preparation and transfer of various buffer solutions using a control unit and controls the concentration and composition of a buffer by transferring a concentrated component solution into the buffer storage tank using an opening/closing valve and a flow rate-adjusting valve, and unlike the related art, reduces the number of buffer preparation tanks corresponding to individual buffer storage tanks, which is advantageous for securing a safe distance between processes and securing a facility area.

An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process), with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.

A method for producing a carbonate-containing species-rich, nitrogen-containing species-free solution from a urea-rich solution is proposed. The method comprising the steps of: providing a first reservoir comprising a first mixture including urea and a catalyser comprising an enzymatic catalyser and/or a microorganism; allowing an enzymatic reaction catalysed by the catalyser to decompose urea, thereby obtaining a second mixture comprising nitrogen-containing species and carbonate-containing species; converting at least some of the nitrogen-containing species into gaseous nitrogen-containing species to obtain a third mixture comprising the gaseous nitrogen-containing species and the carbonate-containing species; filtering the third mixture by a gas- permeable filter, thereby separating at least some of the gaseous nitrogen-containing species from the carbonate-containing species while keeping the catalyser away from the gas-permeable filter; and collecting the so-obtained carbonate-containing species-rich, nitrogen-containing species-free solution.

According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, fertilizer, feed, biofuel manufacture, and desirably impacts, nutrient recovery from waste streams for valued byproducts production, recycle water, and alternative/renewable energy production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal biomass-processing waste or additional algae harvested as feedstock for products such as fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as fertilizer, feed, biofuels, etc. to compete with non-organic or petroleum products in the marketplace.

A bioprocessing system comprising a series of processing stations for performing operations for bioprocessing is disclosed. The bioprocessing system includes an automated system comprising means for manipulating a fluid connection between a first container and a separable second container whereby to create an aseptic connection that enables a controlled transfer of fluid or cell material between the first container and the second container, wherein the means for manipulating a fluid connection is configured to create an aseptic connection that can be disconnected after the transfer of fluid or cell material is complete to enable a further such fluid connection to be manipulated between the first container and a separable third container, and means for controlling an automated sequence of operation of the processing stations.

A waste reduction system that utilizes organic solids suspended in a waste stream to produce carboxylic acids, which can then be employed as an input to a microbial fuel cell or other biological processes to further enhance biogas production, is provided. The organic waste stream influent undergoes a multistage fermentation process in which fermentative microorganism metabolize the organic waste materials and produce one or more carboxylic acids, especially short chain fatty acids. The carboxylic acids serve as a food source for bacteria within an anode compartment of an MFC that generates useable electricity therefrom.

The present invention relates to a process for the fermentation of fungal strains which secrete glucans with a β-1,3-glycosidically linked main chain and side chains β-1,6-glycosidically bonded thereto, in a cascade of tanks using high-shear mixers.

Enzyme production in a bioprocessing facility that utilizes the enzyme that is produced. Enzyme is produced by one or more microorganisms grown on a co-product (e.g., one or more stillage compositions) of the bioprocessing facility.