Described herein is a beads-free bioprocessor as an automated and cost-effective T cell processing and manufacturing platform. T cells are a core component in CAR T cell therapies for cancer treatment, but are difficult to manufacture to scale in clinically relevant quantities. The 3D bioprocessor provides an alternative device that is scalable, beads-free, easy-to-use, and cost-effective for using CAR T cell therapy in cancer immunotherapy. Besides CAR T cell application, this platform technology has potential for many other applications such as cancer cell isolation.

Device, and methods of using or making the device, for engineering cells in vitro are disclosed. In some aspects, a cell culture device comprises at least one glass or polymer surface configured for incubating cells in a culture medium; a charged molecule electrostatically bound to the surface; and a polyelectrolyte multilayer (PEM) electrostatically bound to the charged molecule, the PEM comprising one or more bi-layers of oppositely charged polyelectrolytes, and the PEM having a sufficient thickness to permit release of the charged molecule into the culture medium in a controlled released manner.

The present disclosure relates to methods of preparing personalized blood vessels, useful for transplantation with improved host compatibility and reduced susceptibility to thrombosis. Also provided are personalized blood vessels produced by the methods and use thereof in surgery.

The present disclosure provides a system, including methods and apparatus, for culturing, monitoring, and/or analyzing organoids. In an exemplary method of organoid culture, the method may comprise disposing a scaffold in a receptacle having an open side. A sealing member may be bonded to the open side of the receptacle to create a chamber. An organoid may be formed in the chamber using the scaffold. Fluid and/or at least one substance may be introduced into the chamber from an overlying reservoir for contact with the organoid.

The present disclosure relates to a hydrogel fibre comprising an ionic hydrogel and a second component in a plurality of compartments, wherein the second component is selected from a second hydrogel, a hydrophilic solution, or a mixture thereof. It is also disclosed the method to obtain the aforesaid hydrogel fibres. This disclosure also relates to a composition comprising the hydrogel fibres and a suitable carrier, and an article/kit, a bundle, a mesh or a membrane comprising the hydrogel fibre. A composition comprising an ionic hydrogel and a second component for use in medicine administered in a hydrogel fibre comprising a plurality of compartments is also disclosed.

A microfluidic device includes multiple microfluidic assay units arranged on a substrate, with each assay unit including multiple scaffold regions each containing a three-dimensional scaffold with associated cells, and a media channel surrounding a fluid-permeable boundary portion of at least a second scaffold region, wherein a fluid-permeable interface between the media channel and the second scaffold region comprises a curved shape spanning an arc of more than 90 degrees. A third scaffold region may be provided. Boundaries between different scaffold regions, and between a scaffold region and the media channel, may include microposts that may be spaced apart in a curved configuration. A method for performing an assay utilizing such a device is further provided.

Microfluidic platforms for forming and culturing perfusable hydrogel vascularized tissues typically include one or more culture chambers. Each culture chamber includes at least two openings overlaid over a gel channel. The gel channel typically includes at least two tissue zones and a trapping or insertion portion positioned between the tissue zones. The trapping or insertion portion permits vascular networks to develop between the two tissue zones containing vascularized tissues and/or vascularized tissue masses. The vascularized tissue masses in the tissue zones of the gel channel are connected indirectly, via the vascular network of the trapping portion. Also described are methods of forming and culturing perfusable vascularized tissue masses directly or indirectly interconnected via vascularized networks.

A cell culture device is provided, which comprises a cavity and a base layer, wherein the base layer is a type of plastic thin film and has a thickness of 1 μm to 100 μm; a second-moment of inertia lower than 6×10.sup.6 μm.sup.4; and a resultant flexural rigidity of 1×10.sup.−6 Pa.Math.m.sup.4 to 0.02 Pa.Math..sup.4. Accordingly, the base layer can produce an out-of-plane strain, and bending deformation can occur. Therefore, a growth space close to in vivo environment is provided by the base layer for the cells when the cells attach to the base layer, thereby promoting the growth and maturation of the cells and tissues.

Disclosed herein are synthetic hydrogel useful for the generation, storage and administration of cellular structures such as spheroids and organoids.

Systems and methods are provided for evaluating a tissue that utilize a resistance to represent pressure of a fluid or gas passing through the tissue and a capacitance to represent compliance of the tissue.