The present invention provides a cell culture apparatus. A cell culture apparatus according to one embodiment of the present invention is a cell culture apparatus for culturing a cell to form the cell into a three-dimensional spheroid. The cell culture apparatus may comprise: a plate; and a well which is arranged, in plural, on one side of the plate at predetermined intervals, has cells cultured inside, and has a micropattern disposed on the inner surface such that the inner surface and the cells are spaced apart from each other.

Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.

Disclosed herein are systems, devices, and methods for sterilization. In some variations, a method of sterilizing a bioreactor system may comprise circulating a sterilant in a bioreactor and an enclosed vessel in fluidic communication with the bioreactor. The enclosed vessel may comprise cell culture media. The sterilant may be circulated for a dwell time sufficient to sterilize at least one of the bioreactor, the vessel, and the cell culture media.

Provided herein are models comprising a tissue-like assembly of cells tissues or organoid bodies cultured in the presence of a pulsating alternating ionic magnetic resonance field. The cells, tissues or organoid bodies are introduced into a culture unit comprising growth and nutrient modules in which the gravity vector of the growth unit is continually randomized and cultured in the presence of the alternating ionic magnetic resonance field.

The present disclosure relates to a multi-chamber bioreactor, preferably in a polymeric material with a 3D structure, adapted for cell-mono and co-culture, with at least two entries and outputs of culture medium adaptable to be used as a static culture system and to incorporate a dynamic platform creating a bioreactor. The disclosure also relates to a technique based on a bioreactor device that allows the creation of two or more different tissues integrated with the natural phenotype, using an integrated and continuous 3D support structure.

A cylindrical flow distributor (100) for performing, by means of solid reaction members, a biological or chemical transformation, or physical or chemical trapping from, or release of agents to, a fluidic media is provided. The flow distributor (100) comprises: a top wall (1); a bottom wall (2) comprising a central through going opening (13); and an outer wall (3) extending between the top wall (1) and the bottom wall (2). The top wall (1), the bottom wall (2) and an inner envelope surface (5) of said outer wall (3) together define a confinement (7) configured to contain solid reaction membersor a rigid body of a reaction member material. The outer wall (3) comprises a first plurality of longitudinally extending ribs (20) arranged side by side with longitudinal gaps (21) extending in the circumferential direction between two adjacent ribs (20), and a circumferentially extending first scaffold (22) encircling and being fixedly attached to a peripheral outer surface (29) of said plurality of longitudinally extending ribs (20). Further, a reactor using such flow distributor (100) is provided.

This invention relates to compositions of matter, methods, modules and instruments for automated mammalian cell growth and mammalian cell transduction followed by nucleic acid-guided nuclease editing in live mammalian cells.

The invention relates to a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135−45° to the rotational axis of the centrifugation chamber, wherein the centrifugation chamber comprises at least one input/output port and the cells to be modified are immobilized at the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g.

Furthermore, the invention relates to a method for modifying cells comprising the steps introducing cells in a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135−45° to the rotational axis of the centrifugation chamber wherein and comprising at least one input/output port, immobilizing the cells on the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g maintaining the rotation of the rotation of the centrifugation chamber until the cells are modified.

Methods and systems for processing suspensions of biological cells and microcarriers are disclosed. The biological cells are separated from the microcarriers by introducing the suspension into a spinning membrane separator whereby the biological cells pass through the membrane and the microcarriers do not pass through the membrane.

The present disclosure suggests a food waste treatment apparatus. A food waste treatment apparatus in accordance with an exemplary embodiment of the present disclosure includes: a housing including a food inlet opening at its top surface; an inner chamber where food introduced through the food inlet opening is collected; a stirring unit provided within the inner chamber and configured to stir food waste; an outer chamber positioned outside the inner chamber; and a rotation driving unit configured to rotate the stirring unit and the inner chamber, and the inner chamber and the stirring unit are rotated by the rotation driving unit in opposite directions.