An apparatus and method for preparing and culturing cells comprises a gas permeable chamber having at least an inlet and an outlet for introducing culture medium and cells. A cell growth substrate placed at the end(s) of the gas permeable chamber for cells anchored or embedded. The present invention discloses a simple but efficient cell culture method by slowly rotating the cell culture apparatus horizontally combining with a partially filled culture media in the gas permeable container in order to be able to expose the carriers and submerge the carriers intermittently, to achieve an optimal cell culture environment through the efficient oxygen transfer, CO2 balance, with sufficient nutrient supply, without vigorous agitation to achieve oxygen transfer. The horizontally rotation movement with the present cell growth apparatus efficient the nutrition, carbon dioxide and oxygen transfer during cultured process for production of cellular protein products, viruses or harvesting the cells.

An in situ probe, comprising reusable and disposable components, can be employed to measure cell viability in a rocking bioreactor.

Described herein are methods and systems for cell processing or, more specifically, for introducing various payloads into cells. These methods and systems use a mechanoporation approach in which cells are rapidly compressed and then released to relax while absorbing the payload. More specifically, these methods and systems enable high-throughput mechanoporation with various clogging mitigation features. A cell processing apparatus comprises a shell with an inner shell cylindrical surface, a core with an outer core cylindrical surface, and ridges, supported on and protruding away from one of the inner shell cylindrical surface and the outer core cylindrical surface. The core is disposed inside the shell. The outer core cylindrical surface is concentric with the inner shell cylindrical surface. Each of the ridges forms a ridge gap with the other one of the inner shell cylindrical surface and the outer core cylindrical surface.

Described herein is a modular continuous flow bioreactor for various cells. In one embodiment, the modular cell culture bioreactor apparatus may comprise a plurality of cell chambers disposed between an upper flow chamber and a lower flow chamber; a plurality of lower conduits fluidly connecting the lower flow chamber with one or more lower reservoirs and a plurality of upper conduits fluidly connecting the upper flow chamber with one or more upper reservoirs; one or more pumps fluidly connected through the plurality of conduits with the one or more reservoirs and with the upper and lower flow chambers; wherein each individual cell chamber comprises a lower permeable membrane in fluid communication with the lower flow chamber, a three-dimensional distribution of cells, and an upper permeable membrane in fluid communication with the upper flow chamber.

A device for seeding cells includes a container with a wall, a bottom and a lid. The wall extends between the bottom and the lid. The container can be equipped to be loaded with cells, in particular with cells form a cell suspension. The container defines a rotation axis. The device is further equipped to rotate the container around the rotation axis. The container includes a structured surface that can be arranged at the inner surface of the container. The structured surface has structures equipped to receive the cells. The rotation exerts a (g-)force in direction of the structured surface, such that the g-force acts perpendicular to the structured surface. The exerted force in the direction of the structured surface resembles a g-force required for sedimentation of the cells into the structures.

The present invention concern a method and a device for the isolation of non-magnetic cells from a heterogeneous sample solution containing biological material including desired and undesired cells. The method comprises the steps of: —adding magnetic or magnetizable particles to the sample, wherein said particles have sizes in a range from 100 nm to 4 μm and exhibit surface components which support specific association with target cells, wherein said target cells comprise are either said desired or said undesired cells; —decreasing said external magnetic field gradient; —incubating said sample solution with said magnetic particles to obtain a magnetized cell fraction; —washing said magnetized cell fraction using a washing solution to reduce non-specific binding; —increasing said external magnetic field gradient; —separating said magnetized cell fractionation of target cells from said sample; wherein said sample solution is subjected to an external magnetic field gradient throughout said adding, incubating, washing and separating steps, and wherein said sample solution is rotated at least during said adding, incubating and washing steps.

Rotating suspension culture devices that allow direct microscopy, in situ assays, and automation are disclosed. According to an aspect, a suspension culture device includes a rotatable base having an exterior surface that engages at one or more rollers for rotation of the base about an axis when the at least one roller is turning. The device includes first and second end components attached to the base along the axis. The base and the first and second end components define an interior space for holding liquid. A portion of at least one of the end components is made of a material that is at least partially transparent for viewing into the interior space from outside the base. Further, the device includes ports that each permit fluid communication between the interior space and outside the base.

A cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135-45° to the rotational axis of the centrifugation chamber, wherein the centrifugation chamber comprises at least one input/output port and the cells to be modified are immobilized at the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g. In an embodiment, the device is used as a point-of-care and/or portable device. Further, the present disclosure describes software that, when executed by a processor, causes the device to perform the disclosed functions.

A culture system and a culture device each can minimize the burden on workers and can effectively prevent the effect of temperature changes on culture by performing manipulation of a multilayer culture vessel and the culture in sequence in the same space. The culture system comprises a housing with an internal space in which a multilayer culture vessel including a plurality of trays therein is placed, and a manipulator manipulating the multilayer culture vessel while the multilayer culture vessel is kept in a state placed within the internal space. The multilayer culture vessel is communicated with a liquid supply tube such that a fluid material can be introduced into the multilayer culture vessel from an outside of the housing via the liquid supply tube, or that a fluid can be discharged from the multilayer culture vessel to the outside of the housing via the liquid supply tube.

A centrifugal bioreactor includes a turntable assembly, an electric motor coupled to the turntable assembly, and a replaceable rotor assembly coupled to the turntable assembly. The replaceable rotor assembly includes a plurality of reaction chambers that house cell cultures. A fresh medium tank holds fresh medium for the cell cultures, a spent medium tank holds a first portion of spent medium produced by the cell cultures, and an inoculation tank combines the fresh medium from the fresh medium tank and a second portion of the spent medium. A gas exchanger exposes the fresh medium and the second portion of the spent medium to gas. Additionally, a first pump supplies the fresh medium and the second portion of the spent medium to the plurality of the reaction chambers, and a second pump discard the first portion of the spent medium to the spent medium tank.