The present invention relates to bioreactor system and method thereof wherein support matrix (2) comprises at last one central shaft and plurality of peripheral shaft being radially surrounds central shaft. Arrays of discs (11) are mounted along the shaft by defining interspatial vicinities between two successive plates. Thus, discs mounted on peripheral shafts are rotated within the interspatial vicinity of discs of central shaft to ensures sufficient mixing and avoid stagnant fluidic zones which is created when discs are mounted closely apart from each other on shafts. Further, plurality of deflector vanes that are axially provided along the length of the central shaft to redirect substantially co-axial direction fluid flow into interior of culture vessel and more specifically towards the central axis. Thus, bioreactor system provides scalable and disposable bioreactor with efficient mixing and homogeneous conditions and thereby supports high density growth and maintenance of cells and other biological material.

Rotating suspension culture devices that allow direct microscopy, in situ assays, and automation are disclosed. According to an aspect, a suspension culture device includes a rotatable base having an exterior surface that engages at one or more rollers for rotation of the base about an axis when the at least one roller is turning. The device includes first and second end components attached to the base along the axis. The base and the first and second end components define an interior space for holding liquid. A portion of at least one of the end components is made of a material that is at least partially transparent for viewing into the interior space from outside the base. Further, the device includes ports that each permit fluid communication between the interior space and outside the base.

A multi-position support for a multi-layer cell culture apparatus includes a primary base that rests against a support member in an upright configuration. A support surface is offset vertically from the primary base in the upright configuration. The support surface supports the multi-layer cell culture apparatus with the multi-layer cell culture apparatus located thereon. An intermediate surface extends between the primary base and the support surface. The intermediate surface meets the primary base at an interface that extends at an oblique angle to sides of the primary base. The multi-position support has a tilted configuration where the multi-position support is rotated about the interface such that the support surface is closer to the support member than in the upright configuration with the support surface supporting the multi-layer cell culture apparatus thereon.

A belt for an algal growth system includes a flexible sheet material configured to facilitate growth of a biofilm. The flexible sheet material is mounted on a first frame in a first mounted geometry, the flexible sheet material having a substantially vertical orientation when mounted on the first frame such that a first height of the first mounted geometry is greater than a first width of the first mounted geometry, the flexible sheet material being in contact with a contacting liquid.

This sample treating device 1000 is provided with: a treating stage 110 for treating a sample by mixing the sample and a plurality of solutions; a first motor 812 for rotating the treating stage 110 around a first rotation shaft 810; a second motor for rotating the treating stage 110 around a second shaft 820; and a control unit for controlling the rotations of the treating stage driven by the first rotary motor 812 and the second motor. The control unit combines the operation of rotating the treating stage 110 around the first rotation shaft 810 and the operation of rotating the treating stage 110 around the second rotation shaft, thereby treating the sample.

A large-scale cell culture system is provided. The large-scale cell culture system according to one embodiment of the present invention includes: an incubator that includes an inner space to provides a culture environment in which a cell is stably cultured; a cell culture part that is disposed in the inner space and includes multiple supporters for cell culture disposed therein; a medium supply part that is disposed in the inner space and stores a predetermined amount of medium supplied to the cell culture part; and a pump that is disposed in the inner space, and is connected to the cell culture part and the medium supply part through a connection pipe, respectively, and circulates the medium so that the medium stored in the medium supply part is recovered to the medium supply part after being supplied to the cell culture part, the multiple supporters being provided in a plate shape having a predetermined area, and arranged in a state separated apart from each other at a predetermined distance along a height direction inside the cell culture part.

The present invention relates to a method for in vitro production of red blood cells. The method for in vitro production of red blood cells, according to the present invention, specifies, by cell size, the maturation step of cells exhibiting optimal effects in an agitation-type culture, so that even if an expert does not always identify cell shape, automated processes of culturing are possible, and thus automation in bioreactors is possible in the mass-production of uniform quality and red blood cells.

An automated cell culturing device, which expands, detaches and prepares cells, ready to be implanted in vivo is disclosed. The device is composed by a multi-layered cell culture chamber, a cell preparation chamber, and critical parameters control units which automatically drive the cell culture medium circulation, change and refill. The device according to the invention is characterized in that all the components contacting cells and culture medium constitute a totally disposable “cartridge” in order to avoid cross-contamination and improve safety. The device is particularly useful for expanding and preparing mesenchymal stem cells for osteoarthritis (OA) therapy, and for other cell based therapies in mammals.

The present disclosure provides a system for mimicking the secondary lymphoid organs where suspension cells (e.g., T cells) are expanded; methods el expanding activating, and transfecting the suspension cells in the synthetic, microenvironment, and suspension cells produced by such systems and methods.

The present disclosure provides a capture assembly optionally for use in an automated sample analyzer. The sample analyzer includes an optical assembly for scanning a sample well of a planar substrate, e.g., a multiwell plate that is loaded onto and secured to the capture assembly to perform an assay, e.g., detection of an analyte in the sample. The capture assembly automatically aligns the planar substrate about a rotational axis of a drive shaft and secures the substrate to the drive shaft to prevent unwanted movement or slippage of the substrate during starting, stopping and rotation of the substrate at varying rotational velocities thereby ensuring the sample well is reliably detected by the optical assembly.