A cell evaluation device includes: a porous membrane having a first main face and a second main face; a first passage having a first passage portion facing a first area on which cells are placed in the first main face of the porous membrane; a second passage having a second passage portion facing a second area in the second main face of the porous membrane, the second area being positioned backside of the first area; and a first electrode provided in the first passage portion and a second electrode provided in the second passage portion, the first electrode and the second electrode being positioned across the first area and the second area. In the cell evaluation device, tight junctions are formed among the cells by cell cultivation. With the cell evaluation device, any increase in the electric resistance occurring due to the formation of the tight junctions can be easily measured.

The disclosure relates to a container for filtering a suspension which comprises a lid and a vessel. The container comprises a filter that divides an interior space of the container into a first compartment and a second compartment. The lid comprises a first access and a second access. The first access is connected to the first compartment, and the second access is connected to the second compartment.

A disposable cell enrichment kit includes a crossflow filtration device configured to be disposed along a main loop pathway and to receive a process volume containing a biological sample and utilize crossflow filtration, via a micro-porous membrane, to retain a specific cell population in a retentate from the process volume and to remove a permeate including certain biological components from the process volume. The crossflow filtration device includes a laminated filtration unit that includes the micro-porous membrane, a first mating portion, a second mating portion, and a membrane support. The membrane support includes a first plurality of structural features that define a first plurality of openings, wherein the first plurality of structural features are coupled to the micro-porous membrane and provide support to the micro-porous membrane, and the first plurality of openings allow the permeate to flow through them after crossing the micro-porous membrane.

A cell culture device (1) includes: a culture vessel (10 ) that accommodates a cell suspension containing cells and a culture medium; and a stirring device (30) that is provided at a bottom part of the culture vessel (10) to stir the cell suspension accommodated in the culture vessel (10). The stirring device (30) has a shaft part (32), and a rotating part (33) rotatable with the shaft part (32) as a rotation axis. The rotating part (33) has a hole part (36) into which the shaft part (32) is inserted, and a gap (37) of 100 μm or more is secured between an interior wall demarcating the hole part (36) and the shaft part (32). A closing part (38) that closes an end part of the gap (37) in an axial direction of the rotation axis is provided.

The present disclosure provides advantageous rotary interfaces for fluid assemblies (e.g., rotary interfaces for fluid flow in bioreactor applications), and related methods of fabrication and use. More particularly, the present disclosure provides improved rotary interfaces for fluid flow through porous impellers for filtration and/or sparging for fluid assemblies (e.g., bioreactor applications), and related methods of fabrication and use. Disclosed herein is a fluid assembly (e.g., bioreactor) that includes a porous impeller which is in fluid communication with a hollow shaft that can be used to transport a reaction fluid to an external storage tank or the like. The fluid assembly/bioreactor can include a coupling mechanism that transmits rotary motion from a motor to a primary shaft and then to a hollow secondary shaft, while at the same time permitting removal of a filtrate from the fluid assembly or bioreactor via the hollow secondary shaft and a porous impeller.

The bioenvironmental simulation device according to an embodiment of the present invention includes at least one mounting unit on which cells to be measured are placed, a rotational force application unit configured to rotate the mounting unit so as to apply a rotational force to the cells to be measured placed on the mounting unit, and a culture liquid flow device through which a culture liquid flows across the mounting unit, wherein the culture liquid flows by the culture liquid flow device so as to apply a shear force to the cells to be measured.

A cell culture monitoring system comprising a monitoring apparatus for coupling to a culture tank containing a cell culture medium therein, and a fluid circulation system for fluidic coupling to the cell culture tank the fluid circulation system comprising a dielectrophoresis cartridge for connection to the cell culture tank via supply and return conduits, the dielectrophoresis cartridge comprising a base and an electrode support having electrodes in or on the electrode support, the electrodes configured for traveling wave dielectrophoresis and comprising a measurement zone arranged above a measuring chamber formed between the electrode support and a floor of the base forming a measuring chamber therebetween, whereby cells in a liquid medium flowing through the measuring chamber are subject to a traveling wave dielectrophoresis force orthogonal to a direction of flow of said liquid through said measuring chamber.

A filter apparatus is disclosed for withdrawing a fluid medium from a bioreactor during the growth of a cell culture within the bioreactor. Also disclosed is a method for culturing cells in a bioreactor. The filter apparatus includes a hollow tubular member attached to a filter member. The filter member has a pore size and volume capable of withdrawing a fluid medium at a relatively high flow rate from the bioreactor. Without withdrawing biological cells from the bioreactor and without damaging or harming the cells. The filter apparatus of the present disclosure allows for many process improvements.

An antibiotic susceptibility testing device of gram-negative bacteria, as well as a corresponding method, are discussed. The device has a temperature control unit (including a constant temperature chamber) and a contactless conductivity-based measurement system. Disposable glassy or PVC tubes are used as test vessels for AST. In the performance of AST assay, appropriate kind of liquid medium containing identical amount of target bacterial cells and target antibiotics at different concentrations are loaded into test tubes, following by incubation in the device at a setup temperature. The bacterial growth profile is monitored by collecting the differential values (ΔC) of conductivity of liquid medium, which depend on the proliferation of viable cells. Outcome of ΔC indicates whether the target bacterial cells are completely inhibited by the test antibiotic or not, enabling the user to judge the value of the minimal inhibitory concentration (MIC) simply.

A microorganism sampling device includes: a head part that has a water supply channel to which a water supply pipe for supplying sample water can be connected; a housing part to an upper part of which the head part can be detachably attached; a frame-structured tube; a tubular filter that is arranged within the tubular body; a cup that communicates with the tubular filter and is attached to one end of the tube body; and a cap that has a channel communicating with the tubular filter and is attached to the other end of the tube body. The microorganism sampling device further includes a filter part housed in the housing part, in which a bottom part of the cup is supported by a bottom part of the housing part. The head part is attached to an upper part of the housing part.