C12M29/06

Single use bioreactor

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

PROCESS AND DEVICE FOR THE PRODUCTION OF METHANE

The present invention relates to a process for producing methane and to the device for producing methane, making it possible to increase the methane content of the outgoing gas and preferably simultaneously to increase the methane content of the outgoing gas and the productivity of the reactor.

DEVICE AND METHODS FOR FREE FLOATING MACROALGAE CULTIVATION OFFSHORE

The present invention provides novel devices, systems and methods for cultivating macroalgae in a waterbody, more particularly in the sea/offshore.

FLUID CONDUIT WITH RADIAL EXPANSION OF FLUID FLOW
20220195368 · 2022-06-23 ·

Disclosed herein are systems, devices, and methods for flowing fluid for radially expanded particle distribution within a laminar flow. In some variations, a system for cultivating tissue may comprise a bioreactor comprising an inlet, a substrate arranged in the bioreactor, and a diffusion module configured to transfer fluid from the inlet to the substrate. The diffusion module may comprise a porous material having at least one tortuous conduit extending between a first surface of the porous material and a second surface of the porous material.

BIOREACTOR AND METHOD FOR THE PRODUCTION OF ADHERENT CELL CULTURES EMPLOYING SAID BIOREACTOR
20220177823 · 2022-06-09 ·

Bioreactor and production process of adherent cell cultures that uses the bioreactor. The bioreactor includes a recipient with a cover, and further includes internally a bracket from which protrudes at least one tray that supports a three-dimensional polymer-based element mounted on it, such that when the bioreactor is filled with a culture medium the at least one tray and the three-dimensional polymer-based element are surrounded by the culture medium.

The process includes the following steps: the bracket, with the at least one tray of the bioreactor, with a three-dimensional polymer-based element is immersed in the culture medium, cells that are desired to be planted on the three-dimensional polymer-based element are inoculated from outside the bioreactor, and the culture is left in the bioreactor so that the cellular proliferation is produced.

MICROORGANISM DEODORIZING DEVICE AND DEODORIZATION TREATMENT SYSTEM
20220161189 · 2022-05-26 · ·

[Problem]

To provide a microorganism deodorizing device capable of sufficiently exhibiting a decomposition-deodorization function while suppressing an increase in manufacturing cost, even in a large-scale device including a large-sized deodorizing tank.

[Solution]

A deodorizing tank 1 of a microorganism deodorizing device 1A forms an airflow passage 20 through which air passes from a chamber unit 3 to an opening portion 19; and the airflow passage 20 is provided with a deodorizing unit 5 in which a foam material 17 is filled, a ventilation resistance layer 4 arranged close to or adjacent to the deodorizing unit 5 and configured to increase ventilation resistance of the air flowing through the airflow passage 20, and a chamber unit 3 arranged close to or adjacent to the deodorizing unit 5 and/or the ventilation resistance layer 4 as a chamber which temporarily stores the air fed to the deodorizing tank 1; and the air is fed to the deodorizing unit 5 in a state of being spread in the chamber unit 3 over a substantially entire surface of the deodorizing unit 5 as a result of that the ventilation resistance of flow of the air flowing through the airflow passage 20 is increased by the ventilation resistance layer 4.

BIOREACTOR WITH VOLUME-ADJUSTABLE PACKING LAYER
20220154124 · 2022-05-19 ·

There is provided a bioreactor including: a chamber unit having an accommodating space therein; an upper fixing member and a lower fixing member that are disposed at respective planes perpendicular to a central axis of the chamber unit inside the chamber unit and are movable in a central-axis direction of the chamber unit; and a filling layer that is formed in a space between the upper fixing member and the lower fixing member inside the chamber unit and contains a plurality of carriers.

MANUFACTURING AND USE OF FUNCTIONALIZED SOIL AMENDMENT

Provided is a process and material including the operation of a reactor that applies kinetic energy, heat, and pressure are performed nearly concurrently to biomass feeding of biomass to create functional soil amendment with superior SOM creation and carbon capture.

NOZZLE FOR FLUID DEPLOYMENT IN BIOREACTORS
20230257687 · 2023-08-17 ·

A nozzle system for fluid deployment for treating a biological fluid within a bioreactor, having a bioreactor having an internal volume; an adjustable nozzle deployed within the internal volume; a reservoir capable of containing an agent; and a tubing connecting the reservoir and the adjustable nozzle, wherein the adjustable nozzle is capable of being adjusted to distribute a processing agent in a plurality of distribution streams.

Aerobic fermentation systems and methods

A system for aerobic fermentation includes a vessel, an aeration system including a gas sparger fluidly coupled to the vessel to introduce a compressed gas to an internal volume of the vessel, and a recirculation loop fluidly coupled to an outlet of the vessel. The recirculation loop includes an eductor fluidly coupled to an oxygen-containing gas source, a static mixer downstream of the eductor, a heat exchanger downstream of the eductor, and a distributor downstream of the heat exchanger. The distributor is fluidly coupled to the vessel. The aeration system provides mixing and oxygen mass transfer to the fermentation composition in the vessel. The fermentation composition passes through the eductor, static mixer, heat exchanger, and distributor of the recirculation loop, and back into the vessel. Oxygen is transferred from an oxygen containing gas to the fermentation composition and heat is removed from the fermentation composition in the recirculation loop.