A bioreaction container including a culture chamber configured to contain a culture solution and a life form in an inner space, the culture chamber having an open upper end; a chamber cover portion coupled to the upper end of the culture chamber, the chamber cover portion having a protruding tube provided on one side thereof so as to communicate with the inner space; a filter cap coupled to the protruding tube in an attachable/detachable manner so as to open/close the protruding tube; a gas introduction portion configured to penetrate the chamber cover portion and to communicate with the inner space so as to supply a predetermined gas into the inner space; and an acidity/basicity adjustment portion installed on the chamber cover portion while containing an adjustment solution that adjusts pH of the culture solution such that the adjustment solution is discharged into the inner space by pneumatic pressure.

An apparatus 100 for cultivating bacteria, comprising a conduit 101 having an upstream and a downstream section and the downstream section of the conduit 101 comprising first Venturi eductor means 111 with at least two inlet ports 117, 118 wherein one of said inlet ports 117 is in fluid communication with a supply of nutrient and another one of said inlet ports 118 is in fluid communication with a supply of bacteria such that, in use, nutrient and bacteria are drawn into said Venturi eductor means 111 by a fluid passing along said conduit 101 and said Venturi eductor means 111.

A cell processing system includes at least one processor connectable to a source container filled with a biological fluid, the at least one processor including a spinning membrane configured to receive and separate target cells from the biological fluid, the target cells exiting at a first outlet, one or more containers selectively connected to the first outlet; and, and a magnet. The system also includes a controller coupled to the at least one processor and configured to operate the spinning membrane to receive biological fluid from the source container and to direct the target cells to one of the one or more containers, to pause to permit magnetic particles to be associated with the target cells, and to operate the spinning membrane to receive the contents of one of the one or more containers with the magnet applied to the target cells associated with the magnetic particles.

A device for controlling apical flow to a cell culture includes an apical insert that defines at least one inlet channel extending from an inlet port to an apical feed port and at least one outlet channel extending from an apical effluent port to an outlet port. The apical insert includes a projecting portion configured to extend into a cell culture insert to a depth that is less than a depth of the cell culture insert, and a contact surface configured to maintain a spatial relationship between the projecting portion and the cell culture insert.

A system for oxygenating a biological culture includes a container bounding a compartment and having a top wall, a bottom wall, and an encircling sidewall extending therebetween; a tubular member projecting into the compartment of the container and terminating at a terminal end; a gas supply coupled with the tubular member and being configured to blow gas through the tubular member; and a mixing element disposed within compartment of the container at a location between the terminal end of the tubular member and the bottom wall of the container, the mixing element being configured to mix the liquid.

An inclined reactor of bottom gas-inlet type for aerobic fermentation and a method for aerobic fermentation are provided, a fermenter is provided with a circular inner tank, end covers and a jacket; an airtight fermentation space is formed in the fermenter by the inner tank, an upper end cover and a lower end cover; a feed opening and an exhaust outlet are arranged at an upper part of the fermenter, and a discharge opening is arranged at a lower part of the lower end cover of the fermenter; a length of the fermenter is greater than or equal to a diameter of the fermenter, the fermenter is fixed on a base having a height difference and is hence in an inclined state; an energy-saving stirrer is mounted in the fermenter, and the energy-saving stirrer is formed by connecting several groups of tangential plates or a spiral combination of tangential plates, a radial rod, a stirring rod and a stirring shaft; several groups of air chambers are arranged at an external wall at the bottom of the inner tank of the fermenter, the air chambers are arranged inside the jacket, several aeration nozzles are defined on an inner side of each air chamber, and the aeration nozzles are close to the inner tank.

According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, biofuel manufacture, and desirably impacts alternative/renewable energy production, nutrient recovery from waste streams, and valued byproducts production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal-oil extraction waste or additional algae harvested as feedstock for fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as biofuels, fertilizer, etc. to compete with petroleum products in the marketplace.

Microfluidic devices, systems, and methods providing for an invasion assay using microfluidic culture systems.

A method for removing exhaust gas from a bioreactor (3) and a bioreactor system. The method comprises the steps of: —providing at least one exhaust filter (15; 115a, 115b) connected to an outlet (7) of the bioreactor (3) for transferring exhaust gas out from the bioreactor; —increasing a pressure at an inlet side (25) of the at least one exhaust filter (15; 115a, 115b) in a connection (16) between the bioreactor (3) and the at least one exhaust filter (15; 115a, 115b) or decreasing a pressure at an outlet side (23) of the at least one exhaust filter (15; 115a, 115b).

This invention describes a design for a multiwell plate that contains integrated pumps that are used to stir each well of the plate. The device employs microfluidic logic technology to drive each peristaltic pump. This enables the plates to run autonomously, requiring only a static vacuum supply for power. The devices are entirely constructed out of low-cost polymers, with no electronics, and yet contains simple digital logic circuits to control the pumps. A stack of these plates may be run continuously in a standard cell culture incubator, allowing high-throughput culture of organoids.