The present invention provides a membrane bioreactor for deep-sea cold seeps. The bioreactor is used for simulating a biomembrane growth process during anaerobic oxidation of methane (AOM) in the cold seeps and monitoring changes of environmental conditions thereof. The present invention further provides an online environmental parameter measurement system for a membrane biological reaction in the deep-sea cold seeps. The system includes a fluid supply unit used for generating saturated methane fluid and injecting the saturated methane fluid into the membrane bioreactor for the deep-sea cold seeps at a microflow, and a pressurization system used for ensuring stability and consistency of internal environmental pressure of the system in a simulation process.

A device for continuous dry methanation in a fermenter having a closed tank having at least one compartment to ferment the slurry comprising at least 17% dry matter. An injector to inject pressurized gas close to the bottom of the compartment via at least one chimney that descends through the compartment. The injected gas rising through the slurry creates a convective movement in the slurry around the chimney to stir the matter at the bottom of the compartment. The tank can include a first compartment into which the slurry is introduced and a second compartment into which the hydrolyzed slurry flows after hydrolysis and acidogenesis in the first compartment. Methanogenesis takes place in the second compartment.

A bioreactor system for stem cell expansion has a fluid-filled reactor chamber in which stem cells are cultured. The culture compartment of the reactor chamber is transversed by a perfusion core with multiple niche-like scaffolds positioned along the length of the perfusion core. The bioreactor system also uses a media gas exchange module that is free standing and does not require its inclusion in a CO.sub.2 incubator.

The present invention relates to an apparatus and a method that can accelerate reproduction of odor from an air-conditioner by segmenting microorganisms, a temperature/humidity condition and nutrients for metabolism of the microorganisms as conditions for reproducing the odor from the air-conditioner and setting the segmented microorganisms, a temperature/humidity condition and nutrients for metabolism of the microorganisms according to an accelerated condition thereof.

A micro-incubator manifold for improved microfluidic configurations and systems and methods of manufacture and operation for a manifold and automated microfluidic systems are disclosed. Various embodiments relate to assays, systems, and/or devices for culturing cells or other biologic material in controlled environments and are applicable to related fields generally using microfluidic systems. Particular embodiments involve configurations that can be used with various standard automated handling systems, with active or passive loading and perfusion of medium and to provide high-throughput multi-assay automated systems for culturing, viewing, and analyzing cell growth, invasion, movement, chemotaxis or other properties. More specifically, specific embodiments relate to heat control systems for microfluidic culture plates and other automated systems for culture plates.

Disclosed herein is an improved automatic bioreactor system utilizing a multi-tray or multi-plate cell culture vessel for cultivating adherent cells.

The facility comprises: a first tank (1) comprising separation means (15) extending over a portion of the height of the tank, so as to define a central compartment, or tube (11), a peripheral compartment, or ring (12), and a compartment for stirring and biochemical exchanges (16) in the bottom portion of the tank, comprising stirring means (17), a second tank (2) comprising separation means (25) extending over a portion of the height of the tank, so as to define a central compartment, or tube (21), a peripheral compartment, or ring (22), and a compartment for stirring and biochemical exchanges (26) in the bottom portion of the tank, comprising stirring means (27), means (ALIM) for feeding the waste to be treated into the first ringmeans (T) for transferring the partially treated waste from the first tube to the second ring, means (EVAC) for discharging the treated waste out of the second tube, advantageously pneumatic means (4, 43, 44) for circulating the waste from the first ring to the second tube.

A bag assembly for use with a heat exchanger includes a flexible bag having of one or more sheets of polymeric material, the bag having a first end that bounds a first compartment and an opposing second end that bounds a second compartment, a support structure being disposed between the first compartment and the second compartment so that the first compartment is separated and isolated from the second compartment. A first inlet port, a first outlet port, and a first drain port are coupled with the flexible bag so as to communicate with the first compartment. A second inlet port, a second outlet port, and a second drain port are coupled with the flexible bag so as to communicate with the second compartment.

Organ-on-chip platforms with reduced fluid volumes include circulating fluid volumes of below 1000 ?L, preferably about 500 ?L or less. The platforms are adjustable for culturing cells with varied oxygen demand at various seeding densities. The platforms include at least one lane, wherein each lane includes at least one cell culture well, at least one oxygenator for fluid oxygenation, and a pump system containing at least one pump per lane. The oxygenator may include a separate fluid path for oxygenating the fluid, which allows controlling and measuring the oxygen concentration in the fluid, shortening the diffusion length, and passively diffusing oxygen. Provided are also different configurations for the oxygenator, fluid circulation in the platforms, attachment means for securing scaffolds to culture wells, and pneumatic plates.

Provided herein are systems and methods for recycling and supplementing off-gas from a gas fed reaction process. The systems and methods are particularly useful for bioprocesses that convert hydrogen gas into one or more biosynthetic products. By maintaining separate hydrogen and oxygen feed gas streams, and forming a recycle gas that introduces a target component of the supply gas to the bioreactor within a target concentration range, the yields, productivities, and safety profiles of the bioprocess can be enhanced.