The present disclosure provides methods and compositions for generating mitochondria replaced lymphoid cells, such as T cells, that involves incubating lymphoid cells that have not undergone a procedure to reduce or deplete endogenous mitochondria with isolated exogenous mitochondria and an effective amount of mammalian target of rapamycin (mTOR) inhibitor. In addition, the present disclosure also provides methods of treating an immunological deficiency associated with heteroplasmic immune cells, as well as methods for ameliorating a symptom of mitochondrial complex III deficiency, that involve administering the mitochondria replaced lymphoid cells.

An exchangeable separation insert, a modular centrifugal separator, and a method are disclosed. The exchangeable separation insert includes a rotor casing rotatable about an axis of rotation and a first stationary portion. The exchangeable separation insert includes a ring member which extends concentrically with the axis. The ring member is axially displaceable between a first position, in which the ring member abuts radially against the rotor casing and abuts radially, against the first stationary portion, and a second position, in which the ring member abuts radially against the rotor casing only, or against the first stationary portion only.

Provided is a chamber configuration for a reverse flow centrifuge, and a reverse flow centrifuge system configured for low fluid volume and small radius rotation. The compact reverse flow centrifuge system has a reusable subsystem and a single use replaceable subsystem. The replaceable subsystem comprises a separation chamber, fluid delivery manifold and rotational mounting connecting the separation chamber to the fluid manifold. The single use replaceable subsystem provides a closed environment for execution of reverse flow centrifugation processes. The separation chamber has a substantially conical fluid enclosure portion connected to a neck portion, and a dip tube extends centrally through the conical fluid enclosure to provide a fluid path to the tip of the conical fluid enclosure.

A cell capture apparatus comprises a centrifugal container that can be mounted in a centrifuge, and that has a space in which a test liquid containing target cells is held, and an inner surface that defines the space; and a cell adhesion layer that is disposed on the inner surface and adsorbs the target cells in the test liquid.

The present invention comprises methods and systems for manipulation of media and particles, whether inert materials or biomaterials, such as cells in suspension cell culture. The methods and systems comprise use of an apparatus comprising a rotating chamber wherein the actions of the combined forces of gravity, fluid flow force and centrifugal force form a fluidized bed within the rotating chamber.

A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

A method of manufacturing a culture product liquid includes extracting an intermediate-layer bone marrow liquid positioned in an intermediate layer from bone marrow liquid separated in layered fashion and culturing the intermediate-layer bone marrow liquid with a culture liquid. First stem cells are collected from a first culture container when the total area of the first stem cells reaches a first target ratio of the bottom surface area of the first culture container. Second stem cells are cultured together with the culture liquid. The second stem cells are fixed to the bottom surface of a second culture container, and a culture product liquid including a predetermined metabolite secreted from a single type of second stem cells grown from the second culture container is extracted when the total area of the second stem cells reaches a second target ratio of the bottom-surface area of the second culture container.