A centrifugal bioreactor includes a turntable assembly, an electric motor coupled to the turntable assembly, and a replaceable rotor assembly coupled to the turntable assembly. The replaceable rotor assembly includes a plurality of reaction chambers that house cell cultures. A fresh medium tank holds fresh medium for the cell cultures, a spent medium tank holds a first portion of spent medium produced by the cell cultures, and an inoculation tank combines the fresh medium from the fresh medium tank and a second portion of the spent medium. A gas exchanger exposes the fresh medium and the second portion of the spent medium to gas. Additionally, a first pump supplies the fresh medium and the second portion of the spent medium to the plurality of the reaction chambers, and a second pump discard the first portion of the spent medium to the spent medium tank.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

A centrifuge setup of a bioprocessing installation, wherein, for the centrifugation, the centrifuge setup comprises a centrifuge, wherein the centrifuge comprises at least one centrifuge chamber with a chamber inlet and a chamber outlet, wherein the centrifuge setup comprises a liquid pumping arrangement and a liquid network with a number of liquid lines communicating with the liquid pumping arrangement. The centrifuge setup comprises a sensor arrangement with at least one biomass sensor arrangement, which biomass sensor arrangement is assigned to a liquid line of the liquid network, that the process control calculates an occurrence level of biomass in the respective liquid line based on the sensor signals of the biomass sensor arrangement and that the process control controls the valve arrangement and/or the liquid pumping arrangement during the loading cycle and/or the washing cycle and/or the discharging cycle based on the sensor signals of the sensor arrangement.

An apparatus for separating cell suspension material into centrate and concentrate, includes a single use structure (178, 240, 250) releasably positioned in a cavity in a solid wall rotatable centrifuge bowl (172). The bowl and portions of single use structure rotate about an axis (174). A stationary inlet feed tube (184), a centrate discharge tube (212) and a concentrate discharge tube (230) extend along the axis of the rotating single use structure. A centrate centripetal pump (208) is in fluid connection with the centrate discharge tube. A concentrate centripetal pump (216) is in fluid connection with the concentrate discharge tube. A controller (274) operates responsive to sensors (264, 270) in respective centrate and concentrate discharge lines (262, 268), to control flow rates of a concentrate pump (272) and a centrate pump (266) to produce output flows of cell concentrate and generally cell free centrate.

Methods for preparation of immune cells in a fully closed system are provided. Specifically, the methods contain the steps of pretreating, cell sorting, activation, transduction and expansion. The present methods greatly improve the preparation efficiency of immune cells, and reduce preparation costs.

A clarification setup of a bioprocessing installation wherein, for the centrifugation, the clarification setup comprises a centrifuge, wherein the centrifuge comprises at least one centrifuge chamber with a chamber inlet and a chamber outlet, wherein the clarification setup comprises a liquid pumping arrangement assigned to the centrifuge and a liquid network with a number of liquid lines communicating with the liquid pumping arrangement, wherein the clarification setup comprises a process control for controlling the centrifuge and the liquid pumping arrangement. For filtration, the clarification setup comprises a filter arrangement, that the liquid network provides an outlet supernatant line for a liquid connection between the chamber outlet and the filter arrangement, which is at least temporarily liquid-tight, such that due to this liquid-tight condition of the outlet supernatant line the liquid pumping arrangement as such may drive the supernatant from the chamber outlet to the filter arrangement.

A bioreactor system for culturing cells, comprising: —at least one bioreactor (3); and —at least one hydrocyclone (5a; 5b; 5c; 5d, 5d′; 5e) comprising: —a cell culture inlet (7) which is connected to a cell culture outlet (9) of the bioreactor (3) via a pump (11), whereby a cell culture from the bioreactor can be transferred to the hydrocyclone (5a: 5b; 5c; 5d, 5d′; 5e) when a cell culture is provided in the bioreactor, —a hydrocyclone separation part (13) into which cell culture can be introduced from the cell culture inlet (7), —a top outlet (15) through which a part of the cell culture with decreased cell concentration can be transferred after separation in the hydrocyclone separation pan (13); and —a bottom outlet (17) through which a pan of the cell culture with increased cell concentration can be transferred after separation in the hydrocyclone separation part (13), wherein said bottom outlet (17) of the hydrocyclone (5a; 5b; 5c; 5d, 5d′; 5e) is provided in a position such that when the bioreactor system is used for culturing cells the bottom outlet (17) is in connection with a headspace (21) of the bioreactor (3) and wherein said bottom outlet (17) is provided such that the bottom outlet (17) has a free space around it such that a cell culture transferred out form the bottom outlet (17) is allowed to freely discharge around the bottom outlet.

A microorganism screening system includes a container configured to store a liquid containing microorganism particles and a liquid medium, and a microorganism separation device. The microorganism separation device includes a hydrodynamic separation device and a liquid feeding unit configured to supply the liquid from the container to the hydrodynamic separation device. The hydrodynamic separation device includes a curved flow channel having a rectangular cross-section, and is configured to separate the liquid into a first segment containing relatively large microorganism particles and a second segment containing relatively small microorganism particles through use of a vortex flow generated in the liquid flowing through the curved flow channel. Screening for microorganisms can be performed efficiently.

An exchangeable separation insert, a modular centrifugal separator, and a method are disclosed. The exchangeable separation insert includes a rotor casing rotatable about an axis of rotation and a first stationary portion. The exchangeable separation insert includes a ring member which extends concentrically with the axis. The ring member is axially displaceable between a first position, in which the ring member abuts radially against the rotor casing and abuts radially, against the first stationary portion, and a second position, in which the ring member abuts radially against the rotor casing only, or against the first stationary portion only.

A product concentration device that utilizes a reservoir connected to a hollow-fiber filter element where the reservoir can serve as a container for filtrate emanating from another filtering device, such that product in the reservoir can be stored, concentrated and/or further processed as desired. Enclosed reactor systems, each of at least three chambers, fluid flow between the chambers controlled by selectively permeable barriers, flow controlled by an alternating flow diaphragm pump.