A separating system, for example for separating material from a suspension such as a biological suspension, is disclosed herein. The system comprises a separation vessel arranged to enable the formation of a cyclone therewithin. For example, the separation vessel may be at least partially conical in shape for enabling the formation of a cyclone therewithin. The separation vessel comprises a fluid inlet, an underflow outlet and an overflow outlet. The system also comprises at least one of an underflow outlet fluid control means for controlling the flow of fluid through the underflow outlet, and an overflow outlet fluid control means for controlling the flow of fluid through the overflow outlet. The system may further comprise an inlet fluid control means for controlling the flow of fluid through the fluid inlet.

A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

A centrifugal microfluidic chip is provided that allows an on-chip chamber to provide humidification control, or more generally, gas composition control, to another chamber of the chip. This allows for microfluidic incubation using low-cost and efficient centrifugal devices such as multi-port pneumatic chip controllers, single or multi-port pneumatic slip rings, and articulated centrifugal blades with a pneumatic slip ring. The device may be used for cell culturing, microorganism testing, or production of chemical species from biological samples with a controlled microenvironment.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

The inventions described herein concern improved bioreactors and methods of aerating fluids.

A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

An apparatus for separating cell suspension material into centrate and concentrate, includes a single use structure (178, 240, 250) releasably positioned in a cavity in a solid wall rotatable centrifuge bowl (172). The bowl and portions of single use structure rotate about an axis (174). A stationary inlet feed tube (184), a centrate discharge tube (212) and a concentrate discharge tube (230) extend along the axis of the rotating single use structure. A centrate centripetal pump (208) is in fluid connection with the centrate discharge tube. A concentrate centripetal pump (216) is in fluid connection with the concentrate discharge tube. A controller (274) operates responsive to sensors (264, 270) in respective centrate and concentrate discharge lines (262, 268), to control flow rates of a concentrate pump (272) and a centrate pump (266) to produce output flows of cell concentrate and generally cell free centrate.

A product concentration device that utilizes a reservoir connected to a hollow-fiber filter element where the reservoir can serve as a container for filtrate emanating from another filtering device, such that product in the reservoir can be stored, concentrated and/or further processed as desired. Enclosed reactor systems, each of at least three chambers, fluid flow between the chambers controlled by selectively permeable barriers, flow controlled by an alternating flow diaphragm pump.

The present invention relates to methods of cell culture clarification. In particular the present invention discloses methods of cell culture clarification useful in the manufacturing process of biopharmaceutical molecules, such as antibodies.