A solid-liquid boundary detection device 20 is a device for detecting a boundary 141 between a solid and a liquid in a test tube 14, and includes a surface illumination 21, an imaging unit 22, and a boundary detection processor 31. The surface illumination 21 illuminates the test tube 14 from outside. The imaging unit 22 is arranged on an opposite side of the test tube 14 from the surface illumination 21, and captures an image including the boundary 141. The boundary detection processor 31 detects the boundary 141 from the image captured by the imaging unit 22.

A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

Methods and devices are disclosed for processing stromal precursor cells (i.e., cells which can differentiate into connective tissue cells, such as in muscles, ligaments, or tendons) which can be obtained from fatty tissue extracts obtained via liposuction. Normal processing of a liposuction extract involves centrifugation, to concentrate the stromal cells into a semi-concentrated form called “spun fat”. That “spun fat” can then be treated by mechanical processing (such as pressure-driven extrusion through 0.5 mm holes) under conditions which can gently pry the stromal cells away from extra-cellular collagen fibers and other debris in the “spun fat”. The extruded mixture is then centrifuged again, to separate a highly-enriched population of stromal cells which is suited for injection back into the patient (along with platelet cells, if desired, to further promote tissue repair or regeneration).

Assemblies, systems, and methods for isolation of target material are provided. In various embodiments, an assembly for target material isolation includes a housing having an upper portion and a lower portion together defining an inner chamber. The inner chamber includes a semi-toroidal shape and the semi-toroidal shape defines a longitudinal axis. The assembly further includes one or more fluidic connection from the exterior of the housing to the inner chamber. An isolation material (e.g., polymer wool and/or magnetic beads) may be disposed within the inner chamber. A system includes a configured to fit at least a portion of the housing and releasably couple the assembly. Upon activation of the motor, the assembly may rotate about the longitudinal axis. An angle of the platform may be adjustable to thereby change the angle of the longitudinal axis about which the assembly rotates.

The present invention provides a full-automatic cell production line, comprising a culture region and an operation region. The culture region comprises a B-stage platform body, and the B-stage platform body comprises a culture area, a refrigeration area, and a robot provided with a motion track. The motion track of the robot is linearly arranged. The culture area, the refrigeration area, and a manual delivery window all surround the track, and are all located in the operation region of the robot. The operation region is integrated with a liquid storage table, a cell factory liquid changing device, and a centrifugal bottle liquid changing device. The delivery window is used between the culture region and the operation region to deliver a material. The technical solution of the present invention is mainly used for full-automatic batch production of cells.

A method and apparatus for separating cell suspension into centrate and concentrate includes a solid wall rotatable centrifuge bowl (82). A single use rotatable core (88) is positioned in the bowl and bounds a cavity (90) which serves as a separation chamber. The bowl rotates about an axis (84). An inlet tube (92) and an outlet tube (102) extend along the axis in the bowl cavity. The centripetal pump (98) in the cavity is in connection with the outlet tube. The centrate discharge pump (120) is in connection with the outlet tube. A pressure damping reservoir (122) is fluidly connected between the outlet tube and the discharge pump. At least one control circuit (142) is operative to control the apparatus to maintain a positive pressure above atmospheric pressure within the cavity, so as to maintain the cell suspension away from at least one seal (106) that bounds the interior of the cavity.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Methods and systems are disclosed for manipulating inert materials and biomaterials, including cell cultures, to efficiently form effective cell beds while preventing excess flow through of cells to permeate waste during bioprocessing. Gentle centrifugation concentrates a large volume of cells produced from bioreactors into the desired concentrated volume and cell density. When cells pass through the centrifuge, the majority fraction of cells are retained in the centrifuge disposable chamber pods as a cell bed. A recirculation loop redirects the remaining minority fraction of cells back to the cell bag instead of proceeding to waste. This prevents initial cell loss during cell bed formation in the chamber pods, increases overall cell yields at harvest, and conserves materials, for example. Growing and harvesting natural killer cells, in particular, increased yields by over 30% when the recirculation loop was employed.

The present disclosure relates to hollow fiber tangential flow filters, including hollow fiber tangential flow depth filters, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.