Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel.

A culture container has a first inflow port and a first outflow port. The first flow path connects the first outflow port to the first inflow port. A storage container is provided within the first flow path and has a second inflow port which is connected to the first outflow port and a second outflow port which is connected to the first inflow port. A second flow path connects a first region within the first flow path, and a second region within the first flow path. A division processing portion is provided within the second flow path, performs a division process of dividing a cell aggregation flowing in from the first flow path, and allows the cells subjected to the division process to flow out into the first flow path via the second region. A medium supply portion supplies a medium to the inside of the first flow path.

A digester system comprising a primary digester tank containing a primary feed material portion, a secondary digester tank containing a secondary feed material portion, a first conduit connected between the primary digester tank and the secondary digester tank to define a primary tank lower opening within the primary digester tank and a secondary digester tank lower opening within the secondary digester tank, and a flow control valve configured to allow or prevent flow of fluid through the first conduit. When the flow control valve is configured to allow flow of fluid through the first conduit, a portion of the primary feed material portion flows from the primary digester tank to the secondary digester tank to form the secondary feed material portion.

A cell culture apparatus including cell culture units U and a measurement part (5). The measurement part (5) is provided with an imaging part (51) and is disposed so as to be capable of moving up and down between a back panel part (21) of a casing (2) and a culture container accommodating shelf (32). When the measurement unit (5) reaches a measurement position of each cell culture unit U which is a measurement subject, the culture container accommodating shelf (32) slides toward the measurement unit (5) so that portions to be measured of culture containers U20, U30 in each cell culture unit U come in an imaging range of the imaging part (51).

The invention relates to the use of microjet reactor for processing biomass. The cell lysis of flowable biomass is thereby carried out by means of multiple high-speed liquid jets which collide with one another, wherein the liquid jets contain the cells or consist wholly of the flowable cell mass, wherein intact or wholly or partially lysed biomass is added to at least one of the colliding high-speed liquid jets, and an extraction takes place simultaneously with the collision of the liquid jets or subsequently thereto. The lysis of the cells is initiated or facilitated by the forces that occur on acceleration, introduction of the acceleration, collision of the jets and mixing of the jet constituents.

A microfluidic chip for causing the delivery of a payload to a cell comprises a plurality of constrictions configured to allow a cell suspension to flow through one or more of the plurality of constrictions from a first fluid flow region to a second fluid flow region within the microfluidic chip, wherein a cross-sectional width of each of the plurality of constrictions is less than a diameter of cells in the cell suspension, such that membranes of the cells are perturbed when passing through the constrictions such that a payload is able to pass through the perturbed cell membranes, and wherein a quotient of a cross-sectional area over a cross-sectional perimeter of each of the plurality of constrictions is greater than greater than or equal to 0.5 μm.

A cell culture vessel comprising a housing in which a culture chamber is provided. In the housing, at least two holes that connect the outside of the housing and a culture chamber are provided.

The present disclosure includes systems and methods for hydrolyzing (e.g., pretreatment and/or enzymatic hydrolysis) lignocellulosic biomass into one or more sugars such as pentose and glucose sugars. The present disclosure includes configurations that incorporate flashing and/or liquid cooling so as to permit desirable throughput. The present disclosure also includes a liquefaction configuration so as to permit desirable (e.g., continuous high volume) throughput.

Material processing apparatus, systems, methods, and products are described. An example of a material processing apparatus includes a holding member, a base, a tensioning member, an actuator, and a first inner member. The holding member defines a holding member passageway. The base is attached to the holding member. The tensioning member is partially disposed within the holding member passageway. The tensioning member defines a tensioning member passageway. The tensioning member is moveable relative to the holding member between a first position and a second position. The actuator is attached to the tensioning member and is moveable in a first direction and a second direction. Movement of the actuator results in movement of the tensioning member between its first position and second position. The first inner member is adapted to be disposed within the tensioning member passageway.