A separation device for separating a solid separation target contained in a suspension obtained by suspension culture of adherent cells by using a fine scaffold material, and a separation method. The separation device has a separation chamber having a first chamber and a second chamber, and the first chamber and the second chamber are divided by a mesh structure for separation. The mesh structure for separation has mesh-holes with a predetermined size to suppress passage of the separation target, and a mesh structure for separation is configured in the separation chamber such that the liquid in the aforementioned suspension has a vertically upward directional component in the advancing direction of the liquid when the liquid passes through the mesh-holes.

This biomass treatment system includes: a hydrothermal decomposition section for decomposing the cellulose, hemicellulose and lignin contained in a biomass as a raw material under high-temperature and high-pressure conditions in a main section, namely a tank having a gas-liquid interface, and thus removing a lignin component and a hemicellulose component from the biomass; a discharge section for discharging a biomass solid (a component insoluble in hot-water) obtained by the decomposition; a slurrying tank which is connected to the discharge section and in which the discharged biomass solid is slurried in water fed thereinto to form a biomass slurry; and a solid-liquid separation apparatus including both a settling tank which is provided on a discharge line for discharging the biomass slurry and in which the biomass slurry is settled and a scooping-up and conveying means for scooping up the biomass solid deposited at the bottom of the settling tank and separating the solid biomass component from water.

Provided herein are novel methods for expansion and passaging of cell aggregates comprising stem cells and/or differentiated cells and comprising the use of closed systems in stirred tank bioreactors. The methods of the invention permit closed system serial passage expansion of pluripotent stem cells and/or progeny thereof with associated pluripotency markers and differentiation potential.

Provided is a microfluidic device comprising an incubation layer, the incubation layer including at least one dock, each of the at least one dock defines a stepped tank comprising an upper tank and a lower tank, an inflow channel in fluid communication with the stepped tank for supplying a fluid to the stepped tank, and an outflow channel in fluid communication with the stepped tank for draining the fluid from the stepped tank, wherein the geometry of the upper tank is configured to allow culturing of a fish larva therein, and wherein the geometry of the lower tank is configured to reversibly receive the fish larva from the upper tank and to dock the fish larva at a controlled orientation for imaging or observation.

The present invention provides a system that has been devised to overcome the two most important limitations for sustained biological hydrogen production, namely contamination of the microbial hydrogen-producing cultures with methane-producing cultures necessitating frequent re-start-up and/or other methanogenic bacteria inactivation techniques, and the low bacterial yield of hydrogen-producers culminating in microbial washout from the system and failure. The system includes a continuously stirred bioreactor (CSTR) for biological hydrogen production, followed by a gravity settler positioned downstream of the CSTR, which combination forms a biohydrogenator. The biomass concentration in the hydrogen reactor is kept at the desired range through biomass recirculation from the bottom of the gravity settler and/or biomass wastage from the gravity settler's underflow. The gravity setter effluent is loaded with volatile fatty acids, as a result of microbiological breakdown of the influent waste constituents by hydrogen-producing bacteria, and is an excellent substrate for methane-forming bacteria in the downstream biomethanator.

Provided herein are novel methods for expansion and passaging of cell aggregates comprising stem cells and/or differentiated cells and comprising the use of closed systems on rocking platform bioreactors. The methods of the invention permit closed system serial passage expansion of pluripotent stem cells and/or progeny thereof with associated pluripotency markers and differentiation potential.

The invention relates to a method for treating wastewater by means of photosynthetically active algae that form a sediment in the resting state, comprising the following steps: a) supplying wastewater to photosynthetically active algae that form a sediment in the resting state, b) conveying the wastewater supplied to the algae against gravity from a lower level to a higher level while simultaneously exposing the wastewater mixed with the algae to light, c) introducing the wastewater supplied to the algae from the higher level into the upper region of a sedimentation tank, d) allowing the algae to sediment in the sedimentation tank, and e) removing the wastewater freed of the algae by sedimentation as treated wastewater.

The present invention provides a system that has been devised to overcome the two most important limitations for sustained biological hydrogen production, namely contamination of the microbial hydrogen-producing cultures with methane-producing cultures necessitating frequent re-start-up and/or other methanogenic bacteria inactivation techniques, and the low bacterial yield of hydrogen-producers culminating in microbial washout from the system and failure. The system includes a continuously stirred bioreactor (CSTR) for biological hydrogen production, followed by a gravity settler positioned downstream of the CSTR, which combination forms a biohydrogenator. The biomass concentration in the hydrogen reactor is kept at the desired range through biomass recirculation from the bottom of the gravity settler and/or biomass wastage from the gravity settler's underflow. The gravity setter effluent is loaded with volatile fatty acids, as a result of microbiological breakdown of the influent waste constituents by hydrogen-producing bacteria, and is an excellent substrate for methane-forming bacteria in the downstream biomethanator.

A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.

The invention relates to the use of single- or multilayer plastic web plates in a sloped channel-type solid material separator with a lamella package.