The technology described herein is directed to devices, systems, methods, and assays comprising electrode arrays for electroconductive cells. In particular, the technology generally relates to a microelectrode array (MEA) device comprising both field potential (FP) electrodes and impedance electrodes (IE) that are spatially separated for the functional analysis of the electrical connectivity between at least two cell populations, for example a plurality of neuronal cells and a plurality of contractile cells.

The present invention provides mesh rolled scaffold devices and bioreactor systems that can provide a large surface-to-volume ratio for expanded cell culture. The mesh rolled scaffolds minimize shear stress on cultured cells and support sufficient and uniform mass transfer rates of gases and nutrients. The mesh rolled scaffolds can be connected to a media source via holders in bioreactor systems to support large-scale expansion and maintenance of cell cultures. The present invention also provides the bioreactor systems that can include dialyzers and heat exchangers to modify media and other fluids passing through the systems. The bioreactor systems include media and other fluid reservoirs that can support high stirring rates between about 100 and 10000 rpm, and the overall systems can be pressurized between about 1 and 10 atm to increase gas exchange rates.

A cell stimulation apparatus according to an embodiment of the present invention includes: an electrode unit in which an electrode element is installed; a pMUT element disposed above the electrode unit to be spaced apart from the electrode unit, and configured to generate an ultrasonic wave through a voltage applied thereto; and a well element installed between the electrode unit and the pMUT element and forming a cell chamber together with the electrode unit and the pMUT element, wherein a bio-sample is disposed on the electrode element, and the ultrasonic wave generated by the pMUT element is transmitted to the bio-sample so as to provide ultrasound stimulation to the bio-sample.

Electroporation systems and methods are provided that include a processing assembly including a housing, a lid rotationally connectable to the housing, an opening in a top surface of the housing, an electroporation chamber below the opening in the housing, wherein the electroporation chamber comprises (i) two or more electrodes coated with an electrically conductive, non-cytotoxic material, and (ii) a gasket forming the shape of the electroporation chamber and defining the volume of one or more wells within the electroporation chamber. The system may include a docking station, the docking station comprising, a housing, a port in the housing configured to receive the processing assembly, a lid connected to the housing, one or more contacts configured to connect the docking station to an electroporation system housing.

A cell culture chip has a stack structure formed by sequentially stacking: a first electrode provided on a main surface of a first board; a first partition wall layer including a first main flow path, and a first inlet flow path and a first outlet flow path connected to the first main flow path; a planar mesh structure sheet used as a scaffolding material for cells; a second partition wall layer including a second main flow path, and a second inlet flow path and a second outlet flow path connected to the second main flow path; and a second electrode provided on a main surface of a second board, in which the planar mesh structure sheet is sandwiched between the first partition wall layer and the second partition wall layer, and, among aperture ratios of a surface of the planar mesh structure sheet facing the first partition wall layer, an aperture ratio of a portion facing the first main flow path is greater than an aperture ratios of portions facing the first inlet flow path and the first outlet flow path, and, among aperture ratios of a surface of the planar mesh structure sheet facing the second partition wall layer, an aperture ratio of a portion facing the second main flow path is greater than an aperture ratios of portions facing the second inlet flow path and the second outlet flow path.

A microorganism culture apparatus includes a three-layer stacked structure having a layered culture unit (1) that cultures a microorganism, a layered nutrient supply unit (2) that is arranged on a first surface (11) of the culture unit (1) and supplies a nutrient to the culture unit (1), and a layered environmental component supply unit (3) that is arranged on a second surface (12) and supplies an environmental component to the culture unit (1).

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

A method, apparatus, and system are provided for the printing and maturation of living tissue in an Earth-referenced reduced gravity environment such as that found on a spacecraft or on other celestial bodies. The printing may be three-dimensional structures. The printed structures may be manufactured from low viscosity biomaterials.

The present disclosure relates to methods of stimulating cell proliferation, promoting differentiation of cells, regenerating cells, promoting nodule formation, and promoting myotube formation. The methods include applying one or more pulses of electricity to cells, each pulse of electricity having a duration of between about 10 nanoseconds and about 1,000 nanoseconds, wherein said pulses of electricity are applied under conditions effective to stimulate cell proliferation, promote differentiation of cells, regenerate cells, promote nodule formation, and promote myotube formation.

Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulse width of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.