A cell culture chip includes a plate having a first surface, a first opening formed inside the plate and having one end exposed on the first surface, a second opening formed inside the plate and at a location different from the first opening and having one end exposed on the first surface, a hollow connecting section communicating with the other end of the first opening and the other end of the second opening, and a water repellent section with water repellent treatment being provided at least in the vicinity of the first opening or the second opening on the first surface of the plate.

Microfluidic chips, systems, and methods of making and using thereof are described. The microfluid chip includes a disc-shaped transparent layer containing a plurality of cell traps, pillars, and filers, and a support layer attached thereto. The microfluid chip has at least one inlet port in center of the transparent layer for receiving a sample of liquid and cells, and optionally a plastic cover. The microfluid chip can be designed to be suitable for the forces used for cell pairing/fusion in stationary and spinning format, or suitable for a particular cell fusion method such chemical and electrical methods. The microfluid chip is particularly suited for fusing dendritic cells and tumor cells for immunotherapy, or for generating hybridoma.

Examples of the present disclosure generally relate to systems, methods, and devices for performing electrochemical control and monitoring of bacterial gene expression to a precisely assigned level, and may be used, for example, for controlling the production of a protein of interest.

Therapeutic extracellular vesicles (EVs) containing high copies of functional nucleic acids and other biomolecules are produced in large quantities by laying donor cells on a surface of a chip, adding various plasmids, other transfection vectors and their combinations to a buffer on the chip, applying a pulsulatic electric field across the cells laid on top of the chip surface and plasmids/vectors buffer solution below the chip surface, and collecting the EVs secreted by the transfected cells. The chip surface has a three-dimensional (3D) nanochannel electroporation (NEP) biochip formed on it, capable of handling large quantities of the donor cells. The buffer is adapted for receiving plasmids and other transfection vectors.

The invention is directed to a process to convert carbon dioxide to methane by contacting an aqueous solution comprising dissolved carbon dioxide with an electron charged packed bed comprising of a carrier, suitably activated carbon granules, and a biofilm of microorganisms under anaerobic conditions, wherein more than 90 mol % of the dissolved carbon dioxide in the aqueous solution is present as a bicarbonate ion and/or as a carbonate ion.

The present invention relates to an electroceutical screening device and a screening method using same, respectively, wherein an electrical stimulation is applied, through the electrodes, to the cell clusters received in the channels, and the potentials of the cell clusters are measured so as to measure neural activity, and thus electrical stimulation tests are conducted in various ways, and by measuring conduction velocity according to the various electrical stimulation tests, conditions for electrical stimulation therapy are assessed.

A method of forming a high-throughput, three-dimensional (3D) microelectrode array for in vitro electrophysiological applications includes 3D printing a well plate having a top face and bottom face. A plurality of culture well each includes a plurality of 3D printed, vertical microchannels and microtroughs communicating with the microchannels. The microtroughs and the microchannels are filled with a conductive paste to form self-isolated microelectrodes in each of the culture wells and conductive traces that communicate with the self-isolated microelectrodes.

A fluidic cartridge comprises a fluidic disk having a plurality of alignment openings; a fluidic chip comprising a body, one or more channels formed in the body in fluidic communications with input ports and output ports for transferring one or more fluids between the input ports and the output ports, and a plurality of protrusions formed on the body and received in the alignment openings of the fluidic disk for aligning the fluidic chip to the fluidic disk; an actuator operably engaging with the one or more channels for selectively and individually transferring the one or more fluids through the one or more channels from at least one of the input ports to at least one of the output ports at desired flow rates; and a tube member defining a cylindrical housing for accommodating the fluidic disk, the fluidic chip and the actuator therein.

A flow-through microfluidic apparatus for cell treatment is provided, including a flow chamber and a three-dimensional (3D) microelectrode array disposed in the flow chamber to produce electrical fields for cell railing, electroporation and/or sorting. The flow chamber includes a sample flow region, a first sheath flow region and a second sheath flow region. The sample flow region has an input allowing a sample flow of cells and exogenous agents to enter the sample flow region, a first output allowing damaged cells to exit from the flow chamber. Viable target cells are dielectrophoretic railed along the plurality of 3D microelectrode units; railing cells are electroporated through electrical treatment to intake/uptake of an exogenous agent; and viable treated cells loaded with exogenous agent are dielectrophoretically sorted from cells damaged during the cell treatment.