A kit and a method for improving the quality, acceleration of change in and or improving the yield from a bioreaction of a bioreaction mixture. The bioreaction mixture is typically held in an inner space in a vessel, such as a biomanufacturing vessel, with means for movement of the bioreaction mixture and one or more modules are provided to emit one or more electromagnetic fields to which the bioreaction mixture is exposed over a period of time.

An in vitro three-dimensional multicellular system for maintaining tissue such as heart tissue (e.g. human or other animal heart slices) under physiological conditions is provided. Heart tissue that is cultured using the system remains full viable and functional for at least 6 days. The system is thus suitable to continuously monitor the effects of drugs (e.g. cardiotoxicity) during in vitro testing.

The present invention provides compositions, systems, kits, and methods for combining a. single myeloma cell and a single B-cell (e.g., from an animal exposed to a desired antigen) via discrete entity (e.g., droplet) microfluidics. In certain embodiments, a microfluidic device is used to merge a discrete entity containing a B-cell, and a discrete entity containing a myeloma cell, and a discrete entity containing gellable material, at a merger region via a trapping element in order to generate a combined discrete entity. In further embodiments, the combined discrete entity is treated such that a gelled discrete entity is formed.

Provided herein are systems and methods for electrically stimulating cultured cells, including cardiomyocytes. In particular, provided herein are systems and methods employing reusable electrode arrays with multiwell culture devices that provide electrical stimulation to cells that are cultured in individual wells of the devices. The technologies described may be used to stimulate cells for phenotype analysis.

Embodiments described herein relate generally to devices, apparatuses, and systems with embedded electrodes for rowing, maintaining, and/or using 3D tissues in vitro. The devices, apparatuses, and systems described herein can provide scalable, automated tissue stimulation.

Disclosed are apparatuses, systems, and methods for performing electroporation.

The present disclosure provides systems and methods of electroporation protocol optimization. Embodiments include electroporation machines capable of carrying out test protocols including multiple user-designated parameters. The protocols and parameters can be carried out on samples comprising cells, including portions of a sample, to determine optimum parameters for electroporation for different samples. The systems and methods of optimization preferably use electroporation cartridges, electroporation instruments and systems and methods of electroporation using these devices and systems. In some embodiments, electroporation cartridges comprise an electroporation chamber and electrodes.

Disclosed are flow devices and methods for electroporation, which allow controlling the throughput of electroporation, for example by operating the electroporation process at selected throughput or operating at an increased or decreased level of throughput compared to a reference level of throughput by scaling a subset of electroporation parameters, while allowing maintaining cell viability and transfection efficiency.

Disclosed are microfluidic flow-based electroporation systems that have a flow device, an electrical control module, a fluid delivery module, and a multi-well module. The systems can be used in methods of selecting an electroporation parameter, and in methods of electroporating cells using the selected parameters.

Systems and methods are provided for labelling nucleic acids in a cell of a cell culture or of a tissue. The systems and methods include contacting the cell culture or tissue with a first label and applying an electrical field to the cell, thereby increasing the permeability of the cell membrane, allowing the first label to be introduced into the cell. The cell culture or tissue can be disposed on a multielectrode array, and the electrical field can be applied by operating one or more electrodes of the array that are proximate to (e.g., beneath) the cell in order to control the location of the cell whose permeability is increased and/or to control the timing of such permeability increase.