The present invention provides compositions, systems, kits, and methods for combining a. single myeloma cell and a single B-cell (e.g., from an animal exposed to a desired antigen) via discrete entity (e.g., droplet) microfluidics. In certain embodiments, a microfluidic device is used to merge a discrete entity containing a B-cell, and a discrete entity containing a myeloma cell, and a discrete entity containing gellable material, at a merger region via a trapping element in order to generate a combined discrete entity. In further embodiments, the combined discrete entity is treated such that a gelled discrete entity is formed.

The invention generally relates to containers for cell and tissue culturing with multiple compartments in fluid communication with each other to provide a common culture environment in each of the compartments while maintaining physical separation of cells and tissue therein. The invention further relates to culture containers providing a sterile culture environment with detachably coupleable lids and open access to each compartment within a container.

An integrated microfluidic chip and a single-cell culture, screening, and export method applying the same are disclosed; the chip includes a base, an inlet flow channel, an outlet flow channel, a plurality of common flow channels and a plurality of functional units, wherein two ends of the common flow channel are connected to the inlet flow channel and the outlet flow channel, respectively, wherein each of the functional units includes a single-cell introduction port, a cell culturing-screening chamber, a cell export chamber, a cell export port, and a drive element, wherein the drive element is used to provide power to liquid to introduce single cells entering the common flow channels into the cell culturing-screening chamber, and after culturing and screening, to export target cell population in the cell culturing-screening chamber through the cell export port.

The present invention is related to a biochip and production method thereof. The biochip comprises a carrier, a cell or tissue culture area deposited on the carrier, and a sensor area deposited on the carrier adjacent and fluidly communicating with the cell or tissue culture area. A containing space is contained in the cell or tissue culture area comprising a simulated vascular channel, a cell or a tissue and a culture medium. At least one sensor fixation area is contained at the sensor area for placing a sensor element. The present invention can be a model for stimulating cancer of specific patient to realtimely reflecting the cancer formation, transferring status and treatment strategies. The biochip could also carry testing drugs to observe how the drugs functioning to the cells/tissue as to provide a more accurate instruction of the drugs. The present invention can perform multiple test just within on chip which can save cost and also provide a more accurate test model for the patient.

A microfluidic device in which microfluidic channels are embedded in a culture medium chamber and have open sides. The microfluidic device is patterned with a fluid moved along a hydrophilic surface due to capillary force, and the fluid may be rapidly and uniformly patterned along an inner corner path and a microfluidic channel. In the microfluidic device, the microfluidic channel is connected to facilitate fluid flow with a culture medium through open sides thereof and openings, and thus may provide a cell culture environment in which high gas saturation is maintained. In addition, several microfluidic devices formed on one common substrate are described. Such microfluidic devices may be manufactured of a hydrophilic engineering plastic by injection molding.

A genetically modified cell that can be mass-produced efficiently. The genetically modified cell production system includes a cell-processing isolator for seeding a T cell in a well plate (culture vessel) a cell culture incubator (cell storage) for holding the culture vessel in which the T cell has been seeded and culturing the T cell a virus-processing isolator (nucleic acid preparation isolator) for providing, to the well plate, a virus having a nucleic acid containing a gene to be introduced into a cell a virus storage (nucleic acid storage) for holding the well plate from the virus-processing isolator and a virus infection isolator (nucleic acid introduction isolator) for introducing the nucleic acid into the T cell by infecting the T cell with the virus.

The bioenvironmental simulation device according to an embodiment of the present invention includes at least one mounting unit on which cells to be measured are placed, a rotational force application unit configured to rotate the mounting unit so as to apply a rotational force to the cells to be measured placed on the mounting unit, and a culture liquid flow device through which a culture liquid flows across the mounting unit, wherein the culture liquid flows by the culture liquid flow device so as to apply a shear force to the cells to be measured.

The present invention provides compositions, systems, kits, and methods for analyzing the interaction of T-cells and neoantigen presenting cells (and other cells) via discrete entity (e.g., droplet) microfluids. In certain embodiments, a microfluidic device is used to merge a discrete entity containing a T-cell, and a discrete entity containing a neoantigen presenting cell, at a merger region via a trapping element in order to generate a combined discrete entity. In particular embodiments, at least one thousand of such combined discrete entities are formed in about one second. In some embodiments, whether the receptor on the T-cell sufficiently binds the neoantigen to activate the T-Cell is detected (e.g., via detection of cytokine or granzyme B release). In certain embodiments, provided herein are methods for identifying polyfunctional T-cells or NK-cells, as well as methods of screening for such cells that would be cytotoxic if injected into a subject.

This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells. The disclosed compositions and method entail making “reagent bundles” comprising many (hundreds of thousands to millions) clonal copies of an editing cassette and delivering or co-localizing the reagent bundles with live mammalian cells such that the editing cassettes edit the cells and the edited cells continue to grow.

Disclosed herein are methods of inducing endothelial cell reorganization or differentiation to form micro- and macrovessel structures using an extracellular matrix, such as one derived from small mobile stem (SMS) cells, and a substrate, which can also be coated in molecules or otherwise physically manipulated to cause localized effects on reorganization Also disclosed is a kit implementation for performing endothelial cell reorganization.