An organ-on-chip apparatus includes a first fluid channel, a second fluid channel, and an interface. Respective portions of the first fluid channel and the second fluid channel may extend parallel to and adjacent each other, and the interface may be disposed between the respective portions of the first fluid channel and the second fluid channel such that fluid exchange between the first fluid channel and the second fluid channel is via the interface. The first and second fluid channels may be defined in an extracellular matrix material.

The disclosure relates to methods, systems and compositions for use in the production of milk. More specifically, the disclosure is directed to systems, compositions and methods for in-vitro production of milk using an array of mammary organoids seeded on tertiary-branched, resilient duct scaffolding.

A method for producing tissue constructs comprising two or more distinct regions, each of which comprises a different cell population or lineage is described. The method involves providing a support substrate or substrate assembly comprising two or more physically distinct regions, wherein the two or more physically distinct regions of the support substrate or substrate assembly are different from each other; and depositing/positioning one or more cells on the support substrate or substrate assembly. The cells can form a continuous monolayer with at least two zones, e.g., a proliferative zone and a nonproliferative zone, that can act as in vitro intestinal models. The models are two-dimensional, thus facilitating rapid and facile imaging Systems comprising the tissue constructs and methods of using the constructs to study the effects of pharmaceuticals, uutraceuticais, and metabolites on intestinal cells are also described.

This cell production device comprises: a cell production plate that includes a fluid circuit having a plurality of functional parts integrated therein; and a plurality of closed type connectors that connect the fluid circuit to an external space in a closed manner. The fluid circuit comprises, as the plurality of functional parts: a plurality of injection and discharge units capable of injecting or discharging a plurality of types of fluids into or out of the fluid circuit via the plurality of closed type connectors; a plurality of fluid reservoir units capable of storing the plurality of types of fluids to be injected or discharged; and a cell induction and culture unit that performs at least one of induction and culture of cells based on the stored plurality of types of fluids.

Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.

A method of lithographic masking for spatially localized biochemical stimulus delivery, comprising the steps of providing a group of cells on a substrate, coating a layer of gelatin on a portion of the cells, creating a mask layer on a portion of the layer of gelatin on a portion of the cells, and creating an area of masked cells and an area of unmasked cells. Further, the method can include delivering a biochemical signal to the area of unmasked cells, removing the mask layer, and allowing the cells with the biochemical signal and the cells without the biochemical signal to interact freely.

The present disclosure provides a system, including methods and apparatus, for culturing, monitoring, and/or analyzing organoids. In an exemplary method of organoid culture, the method may comprise disposing a scaffold in a receptacle having an open side. A sealing member may be bonded to the open side of the receptacle to create a chamber. An organoid may be formed in the chamber using the scaffold. Fluid and/or at least one substance may be introduced into the chamber from an overlying reservoir for contact with the organoid.

A microfluidic structure includes a first media channel or well and a second media channel. A removable membrane is provided between the first media channel or well and the second media channel to permit diffusion. One or more plates is used to form the second media channel. A method of creating a microenvironment using the microfluidic structure is also provided.

A device for DNA repair phototherapy is provided. A method of use for the device to provide DNA phototherapy to damaged DNA is also disclosed.

Devices for treatment of cells are disclosed. The devices include an elongated housing and at least one hollow fiber semi-permeable membrane positioned within the housing having a plurality of pores dimensioned to prevent passage of the cells to be treated. Systems for treatment of cells including the device are disclosed. Methods of treating cells, including transducing cells and activating cells, are also disclosed. The methods include introducing a biosample with cells to be treated into the device, introducing media to suspend and release treated cells into the device, and discharging the treated cells from the device.