An incubator for cell and tissue culture under controlled atmospheric conditions has a primary air flow control device that forms a primary, preferably laminar flow, air veil across an opening that allows access to the cells or tissue cultures disposed within the incubator. Preferably, most if not all of the air in the primary (laminar flow) air veil is recirculated, and a secondary air flow control device is used that forms a secondary, preferably laminar flow, air veil between the primary (laminar flow) air veil and a user of the incubator.

A resealable microbial culture and observation device provides a growth platform for the growth, transportation, and inspection of a biologic organism. The device provides a tray having at least one growth medium depression for containing a growth medium and a biologic, at least one anerobic member depression for containing an anerobic member that creates an anerobic environment for the biologic, and a vent to enable filtered gas exchange with the ambient environment, so as to supplement the microbial growth conditions in the growth medium depression during single use or in stacked position of multiple devices; and further having a resealable label with a nontransparent portion for labeling, and a transparent portion for viewing the biologic.

An incubator for cell and tissue culture under controlled atmospheric conditions has a primary air flow control device that forms a primary, preferably laminar flow, air veil across an opening that allows access to the cells or tissue cultures disposed within the incubator. Preferably, most if not all of the air in the primary (laminar flow) air veil is recirculated, and a secondary air flow control device is used that forms a secondary, preferably laminar flow, air veil between the primary (laminar flow) air veil and a user of the incubator.

A culture system and a culture device each can minimize the burden on workers and can effectively prevent the effect of temperature changes on culture by performing manipulation of a multilayer culture vessel and the culture in sequence in the same space. The culture system comprises a housing with an internal space in which a multilayer culture vessel including a plurality of trays therein is placed, and a manipulator manipulating the multilayer culture vessel while the multilayer culture vessel is kept in a state placed within the internal space. The multilayer culture vessel is communicated with a liquid supply tube such that a fluid material can be introduced into the multilayer culture vessel from an outside of the housing via the liquid supply tube, or that a fluid can be discharged from the multilayer culture vessel to the outside of the housing via the liquid supply tube.

This disclosure relates to equipment utilized to manufacture chemical agents, particularly biopharmaceuticals, using Disposable Containers (DCs).

A method for carrying out a reaction process, in particular for setting the mixing and/or aeration of a reaction liquid while the reaction process is being carried out, which includes filling at least one reaction vessel with at least one reaction liquid, wherein an internal volume of the reaction vessel is not completely filled by the at least one reaction liquid at all times during the reaction process, and changing the internal volume of the reaction vessel in the course of the reaction process, in particular in a targeted manner, which causes a movement of the at least one reaction liquid.

Provided is a cell culture apparatus including a culture vessel that stores a cell suspension containing cells; a first filter part that has a first filter membrane that performs membrane separation treatment on the cell suspension extracted from the culture vessel; a first circulation flow path that allows components blocked by the first filter membrane to return to the culture vessel; a second filter part that has a second filter membrane that performs membrane separation treatment on components of the cell suspension permeated through the first filter membrane; a second circulation flow path that allows components permeated through the second filter membrane to return to the culture vessel; and a recovery flow path that recovers components blocked by the second filter membrane. In the cell culture apparatus, an average hole diameter of the first filter membrane is 20 μm or smaller, and 0<B/A≤0.5 is satisfied in a case where an average hole diameter of the first filter membrane is A and an average hole diameter of the second filter membrane is B; or an average hole diameter of the first filter membrane is 20 μm or smaller, and the second filter membrane is an ultrafiltration membrane.

An anaerobic digestion system is provided. The system includes a biomass storage container and a cover positioned over the biomass storage container, where the cover is sealed at an outer edge of the anaerobic digester. Weights may be arranged on the surface of the cover, and the sealed edges may form a water collection region. Additional systems, including thermal management, gas processing, energy storage and recovery, and sensing can also be included.

An incubator for cell and tissue culture under controlled atmospheric conditions has a primary air flow control device that forms a primary, preferably laminar flow, air veil across an opening that allows access to the cells or tissue cultures disposed within the incubator. Preferably, most if not all of the air in the primary (laminar flow) air veil is recirculated, and a secondary air flow control device is used that forms a secondary, preferably laminar flow, air veil between the primary (laminar flow) air veil and a user of the incubator.

The present invention provides a stem cell manufacturing system comprising: a pre-transfer cell solution sending channel 20 through which a solution containing cells flows; an inducer solution sending mechanism 21 which sends a pluripotency inducer into the pre-transfer cell solution sending channel 20; an inducer transfer apparatus 30 which is connected to the pre-transfer cell solution sending channel 20 and transfers the pluripotency inducer into the cells to produce cells harboring the inducer; a cell cluster production apparatus 40 which cultures the cells harboring the inducer to produce a plurality of cell clusters consisting of stem cells; and a packaging apparatus 100 which sequentially packages the plurality of cell clusters.