An improved cell culture monitoring system and method that detects cell growth and concentration in a dynamic environment of incubator/shaker. In order to reduce power consumption and make a wireless cell culture monitoring system practical, several methods of temperature compensation are used to replace a method of controlling the temperature of sensing module. Furthermore its power consumption can be significantly reduced by using an adaptive and synchronized light pulse detection technique.

The present disclosure relates systems for cell culturing, in particular to light-reconfigurable systems including a support substrate, an azobenzene-containing layer and a protective coating layer. The disclosure relates also reversibly inscribing topographies on surface of the azobenzene-containing material of the system, a method for culturing cells on the system and to cell cultures obtainable by the method. Also, a method for producing the system is disclosed.

Provided are methods, devices, and systems for measuring aquatic biomass in an aqueous liquid, optionally for the cultivation, growth optimization, and harvest of an aquatic biomass for plant protein production. In particular, measurements of aquatic biomass density are based on light absorption of the aquatic biomass. The aquatic biomass includes an aquatic organism such as Lemna, including Lemna minor. The plant protein isolates include a RuBisCO protein.

Provided are systems and methods for spatially and temporally controlling light with an illumination device comprising a light source operably connected to a circuit board, one or more light guide plates, one or more optical masks, a controller, and a computer readable medium, comprising instructions that, when executed by the controller, cause the controller to: illuminate a cell or a substrate with light from the light source, and spatially and temporally control illumination of light to the cell or the substrate with one or more illumination parameters, wherein the one or more light guide plates provides uniform illumination of the light. Also provided herein are methods of screening using the system and/or device of the present disclosure.

According to one embodiment, an optical sensor includes a plurality of sensing parts two-dimensionally arranged in a matrix to form a sensor surface, and a phototransmissive sample-supporting plate arranged to be opposed to the sensing parts.

A device which automatically performs a step in which expanded and cultured cells are diluted to a desired cell concentration and re-inoculated using a cell-concentration adjustment device having an inlet for taking in a cell suspension; an outlet for discharging a diluted cell suspension; and a flow path which is provided between the inlet and the outlet and is capable of holding a cell suspension, the flow path being provided with: a liquid delivery pump for causing a cell suspension inside to flow; a cell-concentration measurement instrument for collecting data related to a cell concentration per unit amount of the cell suspension; and a dilution-liquid container for holding a dilution liquid which is supplied to the flow path to dilute the cell suspension. The device further includes a control unit for controlling at least the liquid delivery pump on the basis of the data obtained by the cell-concentration measurement instrument, wherein the control unit determines, on the basis of the data obtained by the cell-concentration measurement instrument, an amount of the dilution liquid required to bring the cell concentration to the desired concentration, and drives the liquid delivery pump so as to take in the required amount of the dilution liquid into the flow path and mix the cell suspension and the dilution liquid.

[Problem] To provide an air particle measurement apparatus and clean environment equipment capable of accurately measuring particles in air even when measuring particles in air having a high humidity.

[Solution] An air particle measurement apparatus connected to a chamber that is temporarily or constantly maintained at a humidity higher than an outside air humidity, includes: a measurement unit configured to measure particles in the drawn gas; a suction pipe through which the gas drawn from an interior of the chamber is transported to the measurement unit, the suction pipe connecting a mounting unit of the chamber and the measurement unit; a pump configured to suction the gas so as to be transported from the chamber to the measurement unit through the suction pipe; and a heating unit configured to heat the gas in a path upstream from the measurement unit.

The invention relates to a method for producing microalgae on a support (14) that is movably mounted essentially in an aqueous medium contained in a tank (12), said method comprising a succession of phases in which the microalgae developing on the support (14) are exposed to sunlight and phases in the shade, the light intensity received in the shade being less than 50% of the average light intensity received during the sunlight exposure phases. The total length of the shade phases is more than 50% longer than the total length of the sunlight exposure phases.

Provided is a cell-culturing apparatus including: an incubator that can maintain an interior thereof in an environment that is appropriate for growth of cells; a cell-culturing vessel that is accommodated inside the incubator; an optical-data acquisition unit that acquires optical data of a medium in the cell-culturing vessel; a medium changing unit that changes the medium in the cell-culturing vessel; and a controller, wherein a timing at which the medium is changed is determined on the basis of a change over time in the optical data acquired by the optical-data acquisition unit.

Methods, systems, and kits for determining a level of dissolved oxygen within a microfluidic device are provided. The microfluidic device can be suitable for cell culture. The methods, systems, and kits can further be used to determine a level of oxygen consumption in a population of biological micro-objects. In particular, the methods, systems, and kits of the present disclosure rely on flowing a fluidic medium containing a dye and a supplied partial pressure of oxygen into the microfluidic device for a period of time, wherein fluorescence emitted by the dye changes based on availability of oxygen proximate to the dye; taking a fluorescence image of an area of interest within the chamber; and correlating fluorescence of the fluorescence image of the area of interest to a reference to determine an observed partial pressure of the oxygen in the area of interest.