The disclosure provides systems and methods for producing a cannabinoid product, which comprises contacting a cannabinoid precursor in a first phase with a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible. The disclosure also provides a composition comprising the cannabinoid precursor in a first phase and a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible.

Methods for determining ethylene concentration in an ethylene oligomerization reactor using an ultrasonic flow meter are described, and these methods are integrated into ethylene oligomerization processes and related oligomerization reactor systems.

The present disclosure relates to a reconfigurable bioprocessing system (80) operable to perform a predetermined set of bioprocessing operations within the bioprocessing system (80), the bioprocessing system (80) comprising: a base unit (60) comprising a plurality of valve actuators, wherein at least one of the plurality of valve actuators is configured to releasably engage with a membrane valve cassette (200, 300, 400) that is removably attachable to the base unit (60), and wherein the at least one of the plurality of valve actuators is actuatable to regulate fluid flow through the membrane valve cassette (200, 300, 400); and a control system (20) operable to selectively actuate respective ones of the plurality of valve actuators to provide a valve configuration within the membrane valve cassette (200, 300, 400) to enable the base unit (60) and the membrane valve cassette (200, 300, 400) together to perform one of the predetermined set of bioprocessing operations.

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

The present invention relates to a cell reprogramming apparatus using energy capable of promoting an environmental influx, and more particularly, the cell reprogramming apparatus has an effect of inducing reprogramming into new types of pluripotent cells having pluripotent characteristics or arbitrary differentiated cells having a different expression type from the differentiated cells by applying energy such as ultrasonic waves, laser, or heat treatment to the differentiated cells.

The invention relates to an industrial bioreactor with a dual cycle-controlled optimization process providing an optimized cultivation process for cell cultures, cell components or metabolic products of the cells in a nutrient medium. The bioreactor comprises a reactor vessel providing controlled bioreactor conditions for the cultivation process, a control unit connected to sensory devices measuring sensory parameter values comprising at least measures related to the composition of the nutrient medium and/or concentration of the nutrient medium and/or oxygen and/or temperature and/or pH-value and/or sterility, and transmitting them to the control unit. The control unit controls and automatically optimizes the operational parameter of the cultivation process and operational parameters of a treatment process applied to the cells during the cultivation process by adjusting operational parameters of the bioreactor affecting the measured sensory parameter values.

A system for algae cultivation, encompassing a set of track segments, load sites, growth lights, sensors, a processor, a heater, and a harvest site. The load sites are designed to transport algae onto the track segments, which facilitate and regulate the flow of liquid, algae, growth medium, and nutrients. The processor is configured to respond to sensor data or set thresholds by adjusting track segments, dispensing nutrients, managing pH balance, and controlling the heater and growth lights. The harvest site is equipped for separating algae from the growth medium through methods like dehydration or filtering, streamlining the harvesting process. This system offers a comprehensive approach to algae cultivation and harvesting.

Systems and methods described herein provide a data presentation, assimilation and monitoring system for a biomanufacturing system utilizing an array of bioreactors for conducting biomanufacturing experiments. In embodiments, the experiments may be controlled by recipes that can be created and altered by credentialed users. During experiments, credentialled users may monitor operational parameters of the bioreactors conducting experiments as well as batch and group parameters across multiple bioreactors. Further, credentialled users may alter recipes to the exclusion of other credentialled users while editing. Further yet, specific checklists may be utilized to ensure that correct and suited equipment (such as bioreactors and experiment bays) are matched with an experiment plan.

A simulation apparatus includes an acquisition unit that acquires a depletion speed at which a protein becomes depleted independently of the cells, a consumption speed at which the cells consume the protein under a first culturing condition, and condition data indicating a second culturing condition that differs from the first culturing condition; a simulation execution unit that simulates a change in a concentration of the protein accompanying propagation of the cells in response to the depletion speed, the consumption speed, and the condition data; and a display unit that acquires, as a result of the simulation, the concentration of the protein and displays whether the concentration of the protein lies within a predetermined range.