The present invention provides an apparatus and methods for producing tetrahydrocannabinolic acid (THCA), cannabichromenic acid (CBCA) and cannabichromenic acid (CBCA) in different ratios. The apparatus comprises: (i) a bioreactor comprising (a) an automated supply system configured to deliver a first automated supply of cannabigerolic acid (CBGA), a cannabinoid acid synthase, and a reaction mixture; and (b) a second automated system to cease the reaction; (ii) a controller configured to modify a property of the reaction mixture to produce the desired products; and (iii) an extractor configured to recover the tetrahydrocannabinolic acid (THCA), cannabichromenic acid (CBCA) or cannabidiolic acid (CBDA) and cannabichromenic acid.

The present invention relates to the field of culturing of biological samples and in one form provides feedback in a biological sample culturing system to improve the viability of biological samples wherein the feedback comprises one or a combination of: measuring and manipulating environmental parameters operatively associated with the biological sample culturing system, and; measuring and manipulating culturing media parameters operatively associated with the biological sample culturing system. In another form the invention provides control of the culturing of biological samples in a biological sample culturing system comprising the steps of: mixing individual culture media components in response to one of a predetermined user selection or a predetermined user profile; dispensing the mixed media components into a preselected culturing pod containing at least one biological sample; providing feedback for the mixing step by measuring one or a combination of environmental parameters and culturing media parameters of the at least one biological sample.

The present invention provides methods for producing cannabinoids and cannabinoid analogs as well as a system for producing these compounds. The inventive method is directed to contacting a compound according to Formula I or Formula II with a cannabinoid synthase. ##STR00001##
Also described is a system for producing cannabinoids and cannabinoid analogs by contacting a THCA synthase with a cannabinoid precursor and modifying at least one property of the reaction mixture to influence the quantity formed of a first cannabinoid relative to the quantity formed of a second cannabinoid.

The present disclosure provides a bioreactor for cell culturing. The bioreactor comprises a container comprising a base and a side wall defining an internal volume for holding a cell suspension. The container is rotatable during use about a rotational axis. The bioreactor also includes a sensor element disposed on an internal surface of the container for interaction with a sensor receiver positioned externally of the container and operable to interact with the sensor element to detect a characteristic of the cell suspension. The sensor element is offset from the rotational axis of the bioreactor and arranged to align with the sensor receiver in at least two rotational positions of the bioreactor during use.

The present disclosure relates to systems, devices, and methods for automated fluid transfer. In an embodiment, the present disclosure relates to a system for fluid transfer between a fluid device and a cartridge for cell processing. The system comprises a first portion comprising an instrument head coupled to a gantry, the instrument head comprising a pneumatic actuator, a peristaltic pump, and a gripping feature for receiving the fluid device. The peristaltic pump may be configured to engage compressible fluidic tubing of a fluid pump module of the fluid device to transfer the fluid between the fluid device and the cartridge. The system further comprises a second portion comprising a docking station for receiving the cartridge. In some variations, movement of the pneumatic actuator relative to the fluid pump module causes occlusion of the compressible fluidic tubing of the fluid pump module by the peristaltic pump.

According to an aspect, a detection device includes: a sensor panel having a detection region provided with sensors that are arranged two-dimensionally and each of which is configured to detect light and generates an output corresponding to a degree of detected light; and a light source having point light sources that are arranged in a light emitting region provided corresponding to the detection region. Each point light source is turned on and the outputs from the sensors are received in first detection processing. Each point light source is turned on at a luminance different from the luminance in the first detection processing and the outputs from the sensors are received in second detection processing. The luminance of the point light source in the second detection processing is based on a relation between the luminance of the point light source and the outputs from the sensors in the first detection processing.

A sensor unit includes a sensor, a probe, and a probe holder. The probe makes contact with electrodes of a connection terminal part of the sensor and applies a specific voltage. The probe holder holds the probe so that the probe protrudes toward the connection terminal part of the sensor, has an opposing surface that is disposed opposite the connection terminal part of the sensor, and forms a non-capillary space between the opposing surface and the connection terminal part of the sensor to allow communication with the space facing the adjacent electrodes of the connection terminal part of the sensor.

An embodiment relates to a method for producing syngas fermentation products using highly active microorganisms, and in particular, to a method for producing syngas fermentation products using highly active microorganisms capable of maximizing production efficiency of fermentation products by obtaining microorganisms having a highly active cell concentration through a biomass boosting step in a bioreactor and using these in a fermentation process in a separate bioreactor.

The present invention provides methods for determining the antimicrobial susceptibility of a microorganism in a clinical sample said method comprising removing a test aliquot from a clinical sample culture before the culture reaches 0.5 McFarland units, isolating the microbial cells and transferring the cells into a suitable culture medium for microbial growth, and performing an AST assay using the isolated microbes, wherein the concentration of microbial cells in the microbial cells used to set up the AST assay is measured before the degree of microbial growth in the different growth conditions of the AST assay is measured. Devices for determining the antimicrobial susceptibility of a microorganism in a clinical sample are also provided.

A device for propagating cells, comprising a housing with a longitudinal orientation extending from a first housing end to a second housing end, a plastic film present in the housing and wound up in a spiral shape around a winding axis oriented along or parallel to the longitudinal orientation, with a first and a second film end and with an inner and an opposite outer side, wherein on the inner side or on the outer side or on the inner and the outer side of the plastic film a multiplicity of spacers is present, arranged and adapted to keep the inner side of the wound up plastic film spaced apart from the outer side in sections or substantially completely in the wound up state, such that a liquid medium can be transferred along the longitudinal orientation. Use of the device for the production of cultivated food or for the production of propagated differentiated or undifferentiated cells.