A bypass flow monitoring chamber that may be retrofit onto the main chamber of an incubation chamber, for continuous collection of environmental samples. This allows for the measurement and correction of the environmental conditions within the chamber, and the return of the sample to the main chamber. Utilizing this sampling method, the separable bypass flow monitoring chamber may be isolated from the main chamber by a set of valves during a period of high-heat decontamination of the incubator therein protecting the sensors and contents of this separable chamber, as well as allowing the use of less expensive and lower temperature rated incubator sensors.

The invention relates to a method and a system for in-situ monitoring of a biochemical process in a reactor comprising a vessel (5) intended to receive a liquid (7), said system comprising: a measuring device (9) intended to be inserted floating into said vessel 5, said measuring device (9) being instrumented with sensors configured to take measurements relating to the biochemical process at successive instants and to transmit, at said successive instants, observation data representing said measurements; and a control device (11) configured to control the regulation of the biochemical reactor (3) at said successive instants, according to said observation data received from the measuring device (9).

An incubator system includes an incubator unit including incubator chamber defined by chamber walls formed of a non-magnetic material; a control unit physically separated from the incubator unit and including operational controls for operation of the incubator system; and at least one duct coupling the incubator unit to the control unit, wherein the incubator system is configured so that the incubator chamber experiences a magnetic field variation of no more than 100 nT arising from the incubator system during incubation operation of the incubation system.

Described herein are novel systems and methods for biomanufacturing, such as tissue cultivation. In some variations the systems and methods may comprise at least one porous substrate.

The present invention relates to bioreactor system and method thereof wherein support matrix (2) comprises at last one central shaft and plurality of peripheral shaft being radially surrounds central shaft. Arrays of discs (11) are mounted along the shaft by defining interspatial vicinities between two successive plates. Thus, discs mounted on peripheral shafts are rotated within the interspatial vicinity of discs of central shaft to ensures sufficient mixing and avoid stagnant fluidic zones which is created when discs are mounted closely apart from each other on shafts. Further, plurality of deflector vanes that are axially provided along the length of the central shaft to redirect substantially co-axial direction fluid flow into interior of culture vessel and more specifically towards the central axis. Thus, bioreactor system provides scalable and disposable bioreactor with efficient mixing and homogeneous conditions and thereby supports high density growth and maintenance of cells and other biological material.

The present disclosure includes systems and methods for hydrolyzing (e.g., pretreatment and/or enzymatic hydrolysis) lignocellulosic biomass into one or more sugars such as pentose and glucose sugars. The present disclosure includes configurations that incorporate flashing and/or liquid cooling so as to permit desirable throughput. The present disclosure also includes a liquefaction configuration so as to permit desirable (e.g., continuous high volume) throughput.

The invention relates to an apparatus (100) for determining properties of a sample (110) arranged in at least one receptacle (130) of a container device (120). The apparatus (100) comprises an actuator (30) which is configured to be coupled to the sample (110) via at least one holding element (34) which is configured to hold the sample (110). Further, the actuator (30) is configured to apply a mechanical stimulus to the sample (110) via the at least one holding element (34). The apparatus (100) comprises a force sensing device (20) which is configured to be coupled to the sample (110) via at least one cantilever (22). Further, the apparatus (100) comprises a frame (1), wherein the actuator (30) and the force sensing device (20) are configured to be mounted to the frame (1), and wherein the frame (1) is configured to be arranged on the container device (120). When the actuator (30) and the force sensing device (20) are mounted to the frame (1) the at least one holding element (34) and the at least one cantilever (22) are arranged in the at least one receptacle (130) when the frame (1) is arranged on the container device (120).

The present disclosure provides a cell separation apparatus for a bioreactor. The cell separation apparatus may be disposed outside the bioreactor and in fluid connection with the bioreactor, the cell separation apparatus may be in a shape of a box body, the cell separation apparatus may include a liquid buffer device including a first liquid cavity disposed in the box body; a filter device including a filter channel and a filter membrane disposed in the box body, the filter membrane may be disposed above the filter channel; and a first liquid channel may be configured in the box body to facilitate a fluid communication between the first liquid cavity and the filter channel. A power system for filtering and microfluidic channels are integrated in the cell separation apparatus that is of a box shape, thereby reducing the volume and production cost thereof.

There is provided a microfluidic system delivering external materials into a cell by cell mechanoporation using inertia, the microfluidic system including a fluidic channel structure through which a solution containing a cell and external materials flows continuously, in which the fluidic channel structure includes a junction between one or more channels, a localized vortex is generated near an interface of the junction, the cell is deformed by the vortex, and transient discontinuities are generated in a cell membrane by the vortex and the external materials are introduced into the cell by solution exchange between the cell and fluid around the cell.

Microfluidic devices with neuronal cells, muscle cells, and optionally other cell types co-cultured therein are provided. Typically one or more the cells has a mutation that contributes to or causes a neuronal or muscular disease or disorder. For example, in some embodiments, one or more of the cultured cells are derived from a subject with a neuronal or muscular disease or disorder. The microfluidic device can facilitate formation of a 3D motor unit and a neuromuscular junction in vitro, and be used to monitor the molecular, biochemical, cellular, and morphological differences in the formation of such structures by healthy and diseased cells, and for testing compounds, dosages of compounds, dosing regimes, and combinations thereof, that may improve or worsen their formation. An exemplary combination drug therapy identified in this way is also provided.