An in vitro endothelial cell culture system for optimizing the pulsatile working mode of a continuous flow artificial heart belongs to the technical field of artificial organs. The system includes three parts: 1) a cell culture model on a microfluidic chip and an off-chip multielement aortic arch afterload fluid mechanics circulation loop; 2) devices for simulating the power source of a cardiovascular system: a fluid loading device is realized by a pulse blood pump, and an artificial heart device is connected in parallel to both ends of the pulse blood pump; and 3) a peripheral detection and feedback control system, comprising pressure and flow sensors, a fluorescence microscope, a CCD high-speed camera system and a proportional-integral-derivative feedback control system. The system can accurately simulate the real hemodynamics microenvironment of vascular endothelial cells in different parts of the aortic arch.

A bioreactor for producing hydrogen gas and other metabolites. The bioreactor utilizes light, fermentation, and other metabolic processes for the production of metabolites, derived from various microorganisms contained within the bioreactor through respective metabolic pathways. The bioreactor comprises a main reactor chamber, a semipermeable membrane, a sleeve, a power supply, a substrate medium, a heating member, a plurality of tubing members, a collection reservoir, a pressure-sealed connecter member, and an agitator.

The invention relates to valve system for controlling a process fluid within a liquid processing system. The valve system comprises a valve arrangement, a pneumatic control system, and a connector unit. When the valve arrangement is connected to the connector unit, two or more valves are formed, such that the pneumatic control system controls an open/close or pressure control mode of the valves. A pump diaphragm system is disclosed, as well as a system for purifying a biological material that comprises the valve system or the pump diaphragm system. Also discloses are methods of using the valve system or the pump diaphragm system in a process for the purification of a biological material.

A full-ocean-depth fidelity enzymological measurement device for microbial extracellular enzyme is provided, comprising a pressure-maintaining sampling bottle, pressure-maintaining transfer equipment, a pressure-maintaining enzymological reactor, and heat preservation equipment and enzyme activity detection equipment. The pressure-maintaining enzymological reactor comprises a barrel body, a plug, polytetrafluoroethylene gaskets, an O-ring, a piston, a high-pressure straight-through valve, and a high-pressure connector. The pressure-maintaining enzymological reactor is in a closed barrel body shape and is internally provided with the piston, and the plug and the valve are arranged at each of two ends of the pressure-maintaining enzymological reactor; and the valve is connected to the pressure-maintaining transfer equipment through the high-pressure connector. According to the full-ocean-depth fidelity enzymological measurement device provided by the present disclosure, full-ocean-depth (0-11000 m) sample pressure-maintaining sampling and transferring can be achieved; and sample collection, transferring and enzymological reaction can be conducted under in-situ pressure and temperature conditions.

An inverted conical bioreactor is provided for growing cells or microorganisms. The bioreactor has an internal space and a perforated barrier within the vessel, through which a liquid may flow, where cells or microorganisms cannot pass through the perforated barrier. The perforated barrier divides the internal space of the bioreactor into a first chamber and a second chamber. Cells are grown within the second chamber and can be perfused by re-circulating the liquid, for example a growth medium, through the bioreactor. Various inlet ports and outlet ports allow controlling the parameters of flow of the growth medium.

A system for injecting a substance into one or more cells of a cell population in a tissue sample, comprising: a robotic manipulator apparatus configured to hold and position a micropipette; an injector controller; a robotic apparatus configured to manipulate a focal plane of a microscope; and a computing device configured to, for each respective cell of the one or more cells of the tissue sample: determine a 3-dimensional location of the respective cell based on images formed by the microscope and captured by a microscope camera; control the robotic manipulator apparatus to insert the micropipette into the respective cell; and control injector controller to eject the substance out of the micropipette and into the respective cell.

In one embodiment, an algae cultivation system includes a basin that contains a liquid and a photobioreactor at least partially immersed in the liquid of the basin, the photobioreactor comprising a closed container including multiple panels that together define an interior space in which algae can be cultivated, at least one of the panels being transparent, the photobioreactor further comprising an inflatable float associated with the container that can be filled with a gas to change one or both of the position and orientation of the container within the liquid.

A cell extruder of the present invention comprises: a pressure vessel in which a sample is dispensed; a regulator for adjusting a set pressure of nitrogen gas injected into the pressure vessel and then maintaining a constant pressure; an input valve for opening and closing the injection of nitrogen gas into the pressure vessel; an exhaust valve for removing the internal pressure of the pressure vessel; a filter holder which is provided with a membrane filter soaked with a reagent, so that the sample in the pressure vessel is fed through by the nitrogen gas and crushed into extracellular vesicles; and a collection container in which the extruded sample is stored. According to the present invention, pressure can be freely adjusted by the regulator. That is, the flow rate of the membrane according to pressure can be controlled, thus allowing the validation of a biopharmaceutical production method using an extracellular vesicle extruder. In addition, during cell extrusion, a sintered disc can be removed and cells can be extruded by using only a membrane. Vesicles can be produced within a fine pressure adjustment range (at a fine low pressure at which the membrane is not torn) in which no sintered disc is required.

A bioreactor sensing system is presented. The bioreactor sensing system comprises a bioreaction vessel. The bioreaction vessel comprises an aperture configured to provide an interface for monitoring contents within the bioreaction vessel. The bioreaction vessel also comprises a port coupled to the bioreaction vessel proximate the aperture. The bioreactor sensing system also comprises a sensing device disposed at least partially within the port such that a sensing element is exposed to the contents. The bioreactor sensing system also comprises an external seal configured to be applied over a portion of the sensing device and a portion of the port.

Systems (700) and methods (800) for method of continuous fluid flow in a bioreactor (700) is provided. The method (800) comprises providing (805) a bioreactor system (700) including a bioreactor volume (720), a filtration part (730), and a recirculation line (721) including a recirculation pump (722) is provided between the bioreactor volume (720) and the filtration part (730). The method (800) further comprises providing (810) a plurality of sensors (760) along the recirculation line (721) and monitoring the fluid flow parameters using the sensors (760). The method further comprises sending (820) a plurality of signals from the sensors (760) indicative of the fluid flow parameters to one or more controllers; and controlling (830) the fluid flow rate at the recirculation pump (722) by means of the or each controller.