An immobilized enzymatic reactor can include a wall defining a chamber having an inlet and an outlet; a solid stationary phase covalently linked to an enzyme and disposed within the chamber; and a pressure modulator in a fluid communication with the chamber and adapted to support continuous flow of a liquid sample comprising a polymer analyte through the inlet, over the solid stationary phase, and out of the outlet under a pressure between about 2,500 and 35,000 psi. In one example, the solid stationary phase includes inorganic/organic hybrid particles in an ultra performance liquid chromatography system, the enzyme is a protease, and the polymer analyte is a polypeptide. The immobilized enzymatic reactor can prepare an analyte for applications such as for hydrogen deuterium exchange mass spectrometry.

A fluid supply interface includes at least one supply coupling configuration, at least one discharge coupling configuration, at least one user coupling configuration, and a fluid conduit that connects the supply, discharge, and user coupling configurations to one another. A supply valve is capable of having fluid flow through it or is blocked for flow through it. A discharge valve is capable of having fluid flow through it or is blocked for flow through it. The supply valve is preloaded into a blocking position that prevents flow, and opens by means of a sufficiently large pressure difference between the two sides of the supply valve, against the preload force, for flow through in a direction from the supply coupling configuration toward the fluid conduit. The discharge valve likewise being preloaded into a closed position that prevents flow through it.

A computer-controlled system designed for multi organ decellularization uses continuous organ perfusion for fast, efficient and reproducible organ scaffold preparation under sterile conditions, allowing organ storage for ready organ regeneration. A single organ version is designed to be used for a single organ recellularization using cell, stem cell and autologous cell of organ receiver solutions for minimal or no immunogenic response, mimicking endogenous organ preparation for patients needing transplantation.

Systems and methods are provided for evaluating a tissue that utilize a resistance to represent pressure of a fluid or gas passing through the tissue and a capacitance to represent compliance of the tissue.

A process for purifying a composition comprising water, a target substance, impurities and optionally cells, the process comprising the steps (A) and (B): (A) preparing a liquid feedstock by performing step (Ai) and/or (Aii) on the composition: (Ai) removing at least some of the cells from the composition; (Aii) concentrating the composition by removing water therefrom; and (B) passing the liquid feedstock through an apparatus comprising at least two processing units, each such unit producing a product stream containing purified target substance and optionally a waste stream comprising at least some of the impurities, wherein each unit comprises specified components (i) to (v). The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

The present invention can provide an incubator having not only a favorable cell culture function but also an autonomous decontamination function that saves the need for connection to an external decontamination device.

The incubator provided with a culture vessel, a circulating means, a temperature-regulating means, and a gas supplying means for supplying a decontamination gas or a humidifying water vapor. The gas supplying means includes a compressed gas generating means, a decontamination solution supplying means, a water supplying means, a mixed gas/liquid regulator, and a vaporizer for generating a decontamination gas or a water vapor. The gas supplying means acts as a decontamination means for supplying the decontamination gas to the air circulating in the circulation passage during an autonomous decontamination before cells are cultured, and the gas supplying means acts as a humidifying means for humidifying the air circulating in the circulation passage when cells are cultured after the autonomous decontamination.

A device for in-vitro modelling in-vivo tissues of organs that includes a first body portion with at least one access chamber, a second body portion with at least one culturing chamber, and a culturing membrane dividing the at least one access chamber from the culturing chamber. The device further includes a third body portion with at least one actuation chamber having at least one limitation cavity, and an actuation membrane dividing the at least one culturing chamber from the at least one actuation chamber. With the device, a robust actuation system can be provided that does not depend on the mechanical properties of the actuation membrane material, nor on the pressure, and that allows to mimic three-dimensional deformations of the tissue, in particular of lung alveoli.

The embodiments of the disclosure provide a biological filtration system and a controlling method for the biological filtration system. The biological filtration system for filtering a solution in a bioreactor, comprises: a filter device having a top end and a bottom end, one of the top end and the bottom end being in fluid communication with the bioreactor for filtering the solution in the bioreactor; a liquid chamber in fluid communication with the other one of the top end and the bottom end for storing a solution in the filter device; a positive pressure pump, in fluid communication with the liquid chamber, for driving a solution to flow from the liquid chamber to the bioreactor; and a negative pressure pump, in fluid communication with the liquid chamber, for driving a solution to flow from the bioreactor to the liquid chamber.

A liquid filtration system according to the present invention comprises a bioreactor; a filter configured to filter liquid passing therethrough; a perfusion pump configured to move liquid between the bioreactor and the filter; a liquid line providing fluidic communication between the bioreactor, perfusion pump and filter; and a bleed outlet provided on the liquid line, the bleed outlet configured to provide means to remove liquid selectively from the system under the action of the perfusion pump. With the liquid filtration system according to the present invention, both the filtering and bleed functions may be performed under the action of a single pump, thereby significantly reducing the complexity and cost of the components required.

A bioproduction mixer control system includes a mixing compartment having a biological fluid and a mixing element; a sensor in communication with the mixing compartment, the sensor configured to detect an environmental condition within the mixing compartment; and an integrated control unit. The integrated control unit includes a memory for storing the environmental condition, sensor information relating to the sensor, and process workflow information; a user interface comprising a display depicting a bioproduction workspace, wherein the bioproduction workspace comprises a process workflow title and a plurality of bioprocess modules; and a central processing unit in electronic communication with the sensor, memory and user interface.